A series of different crystalline phases BiVO4 photocatalysts (tetragonal, monoclinic, and monoclinic/tetragonal heterophase) have been prepared by a coprecipitation and molten salt method. ...High-resolution transmission electron microscopy (HRTEM) results show that an interface of intimate contact is formed in monoclinic/tetragonal heterophase and monoclinic phase is mainly on the surface of nanoparticles. Surface photovoltage (SPV) and transient photovoltage (TPV) techniques are used to further investigate the transfer process of photoinduced charge carriers. The results show that the behavior of photoinduced charges markedly depend on the crystalline phases of BiVO4 samples, and the presence of interface in monoclinic/tetragonal heterophase provides a spatial condition for charge transfer, promotes the separation of photoinduced electron–hole pairs, and changes the migration direction of photoinduced carriers. The relationship between behavior of photoinduced charge carriers and photocatalytic activity was discussed in detail, which would provide a greater insight into the intrinsic reasons of the enhancement in photocatalytic activity.
L. is rich in phenolic components and it has natural antioxidant characteristics which are important to human health. In the present study, the total phenolic and flavonoid contents of developing ...seeds of yellow- and black-seeded
were compared. Both phenolic and flavonoid contents were significantly higher at 5 weeks after flowering (WAF) in black seeds (6.44 ± 0.97 mg EE/g phenolics and 3.78 ± 0.05 mg EE/g flavonoids) than yellow seeds (2.80 ± 0.13 mg/g phenolics and 0.83 ± 0.01 mg/g flavonoids). HPLC⁻DAD⁻ESI/MS analysis revealed different content of 56 phenolic components between yellow and black-seeded
, including kaempferol-3-
-glucoside, isorhamnetin-3-
-glucoside, quercetin-3-
-sophoroside, procyanidin B2 (DP 2), which were significantly reduced in yellow seeds compared with black seeds. Applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical assay, we found maximum clearance of DPPH and ABTS in the late developmental stages of yellow and black seeds. Additionally, the ferric reducing antioxidant power (FRAP) value maximized at 5 WAF in black seeds (432.52 ± 69.98 μmol Fe (II)/g DW) and 6 WAF in yellow seeds (274.08 ± 2.40 μmol Fe (II)/g DW). Generally, antioxidant ability was significantly reduced in yellow-seeded
compared to black rapeseed, and positive correlations between antioxidation and flavonoid content were found in both yellow- and black-seeded
.
Aim: To investigate how ecosystem water-use efficiency (WUE) varies spatially under different climate conditions, and how spatial variations in WUE differ from those of transpiration-based water-use ...efficiency (WUEt) and transpiration-based inherent water-use efficiency (IWUEt). Location: Global terrestrial ecosystems. Methods: We investigated spatial patterns of WUE using two datasets of gross primary productivity (GPP) and evapotranspiration (ET) and four biosphere model estimates of GPP and ET. Spatial relationships between WUE and climate variables were further explored through regression analyses. Results: Global WUE estimated by two satellite-based datasets is 1.9 ± 0.1 and 1.8 ± 0.6 g C m⁻² mm⁻¹ lower than the simulations from four process-based models (2.0 ± 0.3 g C m⁻² mm⁻¹) but comparable within the uncertainty of both approaches. In both satellite-based datasets and process models, precipitation is more strongly associated with spatial gradients of WUE for temperate and tropical regions, but temperature dominates north of 50° N. WUE also increases with increasing solar radiation at high latitudes. The values of WUE from datasets and process-based models are systematically higher in wet regions (with higher GPP) than in dry regions. WUEt shows a lower precipitation sensitivity than WUE, which is contrary to leaf- and plant-level observations. IWUEt, the product of WUEt and water vapour deficit, is found to be rather conservative with spatially increasing precipitation, in agreement with leaf- and plant-level measurements. Main conclusions: WUE, WUEt and IWUEt produce different spatial relationships with climate variables. In dry ecosystems, water losses from evaporation from bare soil, uncorrelated with productivity, tend to make WUE lower than in wetter regions. Yet canopy conductance is intrinsically efficient in those ecosystems and maintains a higher IWUEt. This suggests that the responses of each component flux of evapotranspiration should be analysed separately when investigating regional gradients in WUE, its temporal variability and its trends.
Ideally, the inner (the upper or lower arm) current of a modular multilevel converter (MMC) is ideally assumed to be the sum of a dc component and an ac component of the fundamental frequency. ...However, as ac current flows through the submodule (SM) capacitors, the capacitor voltages fluctuate with time. Consequently, the inner current is usually distorted and the peak/RMS value of it is increased compared with the theoretical value. The increased currents will increase power losses and may threaten the safe operation of the power devices and capacitors. This paper proposes a closed-loop method for suppression of the inner current in an MMC. This method is very simple and is implemented in a stationary frame, and no harmonic extraction algorithm is needed. Hence, it can be applied to single-phase or three-phase MMCs. Besides, this method does not influence the balancing of the SM capacitor voltages. Simulation and experimental results show that the proposed method can suppress the peak and RMS values of the inner currents dramatically.
Successive evidence has established that maltol, a flavor‐enhancing agent, could provide resistance to oxidative stress‐induced tissue injury in various animal models though its benefits for ...aging‐induced liver and kidney injuries are still undetermined. In the present work, for demonstrating maltol's ameliorative effect and probable mechanism against aging‐induced liver and kidney injuries, D‐galactose (D‐Gal)‐induced animal in vivo and HEK293 cells in vitro models were established and results demonstrated that long‐term D‐Gal treatment increases the accumulation of advanced glycation end products (AGEs) in liver and kidney tissues, mitigates cell viability, and arrests the cycle. Interestingly, 4‐weeks maltol treatment at 50 and 100 mg/kg activated aging‐associated proteins including p53, p21, and p16 followed by inhibiting malondialdehyde (MDA)'s over‐production and increasing the levels of antioxidant enzymes. Therefore, decreases in cytochrome P450 E1 (CYP2E1) and 4‐hydroxydecene (4‐HNE)'s immunofluorescence expression levels are confirmed. Furthermore, maltol improved oxidative stress injury by activating the phosphatidylinositol‐3‐kinase (PI3K)/protein kinase B (Akt) signaling pathway. In conclusion, the purpose of the present study was to estimate the mechanistic insights into maltol's role as an antioxidant in liver and kidney cell senescence and injury, which will reflect potential of therapeutic strategy for antiaging and aging‐related disease treatment.
We report an event of two‐satellite measurements of simultaneous earthward and tailward fast flows of ~500 km/s in the midtail at X ~ −63 RE and evaluate magnetic reconnection as a responsible ...mechanism by comparing the observations with a particle‐in‐cell (PIC) simulation. The two satellites were near midnight separated mainly along the X direction by ~5 RE. As they moved across the current sheet from the northern to southern lobes, the one closer to the Earth crossed the x line with fast flows changing from tailward to earthward, while the other one simultaneously observed tailward flows. The observed plasma and fields showed several key reconnection signatures, including the Walén relation, the fast reconnection rate of ~0.1, the Hall magnetic and electric fields, and counterstreaming electrons in the separatrix, indicating the fast flow was the reconnection exhaust. The observed temporal variations of flow speeds and magnetic fields suggested that the x line was moving tailward to a location between the two satellites and the exhaust was moving up and down. Within the exhaust, plasma pressure was highly anisotropic, and the current sheet can be unstable to the mirror, ion cyclotron, and firehose instabilities. Current sheet flapping and enhanced compressional waves near proton's local gyro frequencies were observed around the current sheet. Comparing with the PIC simulation suggests that the waves were mainly a result of oblique firehose instability.
Key Points
Earthward and tailward fast flows were observed simultaneously by two ARTEMIS probes in midtail
The characteristics of the fast flows are consistent with the reconnection exhaust
Enhanced compressional ULF waves observed within the fast flow were likely a result of oblique firehose instability
Extracellular vesicles (EVs), including exosomes and microvesicles, are present in a variety of bodily fluids, and the concentration of these sub-cellular vesicles and their associated biomarkers ...(proteins, nucleic acids, and lipids) can be used to aid clinical diagnosis. Although ultracentrifugation is commonly used for isolation of EVs, it is highly time-consuming, labor-intensive and instrument-dependent for both research laboratories and clinical settings. Here, we developed an integrated double-filtration microfluidic device that isolated and enriched EVs with a size range of 30-200 nm from urine, and subsequently quantified the EVs via a microchip ELISA. Our results showed that the concentration of urinary EVs was significantly elevated in bladder cancer patients (n = 16) compared to healthy controls (n = 8). Receiver operating characteristic (ROC) analysis demonstrated that this integrated EV double-filtration device had a sensitivity of 81.3% at a specificity of 90% (16 bladder cancer patients and 8 healthy controls). Thus, this integrated device has great potential to be used in conjunction with urine cytology and cystoscopy to improve clinical diagnosis of bladder cancer in clinics and at point-of-care (POC) settings.
Summary
Arsenic can be biomethylated to form a variety of organic arsenicals differing in toxicity and environmental mobility. Trivalent methylarsenite (MAs(III)) produced in the methylation process ...is more toxic than inorganic arsenite (As(III)). MAs(III) also serves as a primitive antibiotic and, consequently, some environmental microorganisms have evolved mechanisms to detoxify MAs(III). However, the mechanisms of MAs(III) detoxification are not well understood. In this study, we identified an arsenic resistance (ars) operon consisting of three genes, arsRVK, that contribute to MAs(III) resistance in Ensifer adhaerens ST2. ArsV is annotated as an NADPH‐dependent flavin monooxygenase with unknown function. Expression of arsV in the arsenic hypersensitive Escherichia coli strain AW3110Δars conferred resistance to MAs(III) and the ability to oxidize MAs(III) to MAs(V). In the presence of NADPH and either FAD or FMN, purified ArsV protein was able to oxidize both MAs(III) to MAs(V) and Sb(III) to Sb(V). Genes with arsV‐like sequences are widely present in soils and environmental bacteria. Metagenomic analysis of five paddy soils showed the abundance of arsV‐like sequences of 0.12–0.25 ppm. These results demonstrate that ArsV is a novel enzyme for the detoxification of MAs(III) and Sb(III) and the genes encoding ArsV are widely present in soil bacteria.
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