Abstract
Quantum computing, which is based on the rules of quantum mechanics, has great potential in many fields for its theoretical ability to simulate any natural process based on quantum ...mechanics. Many types of physical implementations of quantum computers have been developed, and the method based on trapped ions is a promising one. However, there is still a lot of work that need to be done, like how to improve the fidelity and scalability of those quantum computers. Besides laser light, microwave and waveguides have also been used in some experiments.
Objective
The purpose of this study was to investigate the relationship between work passion and work engagement among university music teachers in flow experience and teaching well-being.
Methods
...Three hundred forty-three university music teachers were tested by using the Flow State Scale, Teacher Well-Being Scale, Work Passion Scale, and Work Engagement Scale.
Results
University music teachers’ flow experience can predict teaching well-being (
β
= 0.248,
p
< 0.001). University music teachers’ flow experience has an indirect impact on teaching well-being through work passion (
β
= 0.257,
p
= 0.005), and university music teachers’ flow experience has an indirect impact on teaching well-being through work engagement (
β
= 0.144,
p
= 0.018). In addition, work passion and work engagement play a chain-mediating role between university music teachers’ flow experience and teaching well-being (
β
= 0.134,
p
= 0.001).
Conclusion
Work passion and work engagement play a sequential mediating role between university music teachers’ flow experience and teaching well-being.
Automobile exhaust always makes up a significant portion of the causes of air pollution, and new energy vehicles (NEV) are being investigated as a potential solution to the problem of urban air ...pollution. Through a number of policy incentives and infrastructure improvements, the Chinese government encourages the quick development of the new energy automotive sector. National air quality has also improved dramatically in recent years. In this context, the primary goal of this study is to explore whether there is a significant positive correlation between the popularization of NEVs and air quality. This study looks for data on the penetration rate of NEVs in various provinces and cities across the country, as well as the data on the number of days with air quality, and makes a visual chart to show the change trend from 2013 to 2022. Finally, policy recommendations are given. The government should adapt to local conditions and continue to boost the infrastructure of NEVs. In addition, clean energy power generation, sustainable production and recycling should be actively promoted from the perspective of the whole life cycle of NEVs.
Heavy metal (HM) contamination in sediments of Three Gorges Reservoir (TGR) is a particularly important issue for the safety of water quality due to the potential threats of metal toxicity to local ...and downstream human health. Surface sediments from riparian and submerged areas in the entire TGR mainstream were collected in 2014 to investigate the spatial distribution of HMs (Cd, Cu, Pb, and Zn), identify their possible sources, and assess their potential risk by multiple indices and metal fraction. Results showed that the concentrations of HMs in the sediments increased after the TGR operation, but were lower than those in other Chinese rivers of developed areas. The acid-soluble Cd accounted for more than 50% of total Cd in the sediments, whereas that of other HMs was very low. The Cd concentrations in the riparian sediments increased towards the dam; however, other metals in the riparian sediments and all HMs in the submerged sediments did not show any regular variation trend spatially. The stocks of HMs were significantly higher in the submerged sediments than in the riparian sediments. The high accumulation of HMs in the riparian sediments emerged between Fuling and Fengjie, and those in the submerged sediments existed in the near dam areas. Grain size and Fe/Mn oxides controlled the mobility and transfer of HMs in the sediments. Human activity in the catchment including industrial and agricultural production, shipping industry, mining, etc., increased inputs of HMs in the sediments, and altered their spatial distribution patterns. The sediments were moderately to highly contaminated by Cd, and slightly contaminated by other HMs. The results indicate the current priority of Cd contamination in the TGR, and will conduce to ecological protection in the TGR region.
Display omitted
•Distribution of Cd, Cu, Pb and Zn in sediments of the entire TGR mainstream was investigated.•HMs stocks concentrated in middle riparian zones and near dam submerged areas.•Sediment grain size and chemical conditions determined HMs mobility.•TGR operation and local human activity controlled HMs distribution in sediments.•TGR mainstream sediments were moderately to highly contaminated by Cd.
Human activity in the catchment of Three Gorges Reservoir increased heavy metal accumulation in the riparian and submerged sediments.
Notwithstanding the numerous drugs available for liver cancer, emerging evidence suggests that chemotherapeutic resistance is a significant issue. HGF and its receptor MET play critical roles in ...liver carcinogenesis and metastasis, mainly dependent on the activity of receptor tyrosine kinase. However, for unknown reasons, all HGF-MET kinase activity-targeted drugs have failed or have been suspended in clinical trials thus far. Macroautophagy/autophagy is a protective 'self-eating' process for resisting metabolic stress by recycling obsolete components, whereas the impact of autophagy-mediated reprogrammed metabolism on therapeutic resistance is largely unclear, especially in liver cancer. In the present study, we first observed that HGF stimulus facilitated the Warburg effect and glutaminolysis to promote biogenesis in multiple liver cancer cells. We then identified the pyruvate dehydrogenase complex (PDHC) and GLS/GLS1 as crucial substrates of HGF-activated MET kinase; MET-mediated phosphorylation inhibits PDHC activity but activates GLS to promote cancer cell metabolism and biogenesis. We further found that the key residues of kinase activity in MET (Y1234/1235) also constitute a conserved LC3-interacting region motif (Y1234-Y1235-x-V1237). Therefore, on inhibiting HGF-mediated MET kinase activation, Y1234/1235-dephosphorylated MET induced autophagy to maintain biogenesis for cancer cell survival. Moreover, we verified that Y1234/1235-dephosphorylated MET correlated with autophagy in clinical liver cancer. Finally, a combination of MET inhibitor and autophagy suppressor significantly improved the therapeutic efficiency of liver cancer in vitro and in mice. Together, our findings reveal an HGF-MET axis-coordinated functional interaction between tyrosine kinase signaling and autophagy, and establish a MET-autophagy double-targeted strategy to overcome chemotherapeutic resistance in liver cancer.
Abbreviations: ALDO: aldolase, fructose-bisphosphate; CQ: chloroquine; DLAT/PDCE2: dihydrolipoamide S-acetyltransferase; EMT: epithelial-mesenchymal transition; ENO: enolase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GLS/GLS1: glutaminase; GLUL/GS: glutamine-ammonia ligase; GPI/PGI: glucose-6-phosphate isomerase; HCC: hepatocellular carcinoma; HGF: hepatocyte growth factor; HK: hexokinase; LDH: lactate dehydrogenase; LIHC: liver hepatocellular carcinoma; LIR: LC3-interacting region; PDH: pyruvate dehydrogenase; PDHA1: pyruvate dehydrogenase E1 alpha 1 subunit; PDHX: pyruvate dehydrogenase complex component X; PFK: phosphofructokinase; PK: pyruvate kinase; RTK: receptor tyrosine kinase; TCGA: The Cancer Genome Atlas
Evidence of whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes coronavirus disease 2019 (COVID-19), can be transmitted as an aerosol (ie, airborne) has ...substantial public health implications.
To investigate potential transmission routes of SARS-CoV-2 infection with epidemiologic evidence from a COVID-19 outbreak.
This cohort study examined a community COVID-19 outbreak in Zhejiang province. On January 19, 2020, 128 individuals took 2 buses (60 46.9% from bus 1 and 68 53.1% from bus 2) on a 100-minute round trip to attend a 150-minute worship event. The source patient was a passenger on bus 2. We compared risks of SARS-CoV-2 infection among at-risk individuals taking bus 1 (n = 60) and bus 2 (n = 67 source patient excluded) and among all other individuals (n = 172) attending the worship event. We also divided seats on the exposed bus into high-risk and low-risk zones according to the distance from the source patient and compared COVID-19 risks in each zone. In both buses, central air conditioners were in indoor recirculation mode.
SARS-CoV-2 infection was confirmed by reverse transcription polymerase chain reaction or by viral genome sequencing results. Attack rates for SARS-CoV-2 infection were calculated for different groups, and the spatial distribution of individuals who developed infection on bus 2 was obtained.
Of the 128 participants, 15 (11.7%) were men, 113 (88.3%) were women, and the mean age was 58.6 years. On bus 2, 24 of the 68 individuals (35.3% including the index patient) received a diagnosis of COVID-19 after the event. Meanwhile, none of the 60 individuals in bus 1 were infected. Among the other 172 individuals at the worship event, 7 (4.1%) subsequently received a COVID-19 diagnosis. Individuals in bus 2 had a 34.3% (95% CI, 24.1%-46.3%) higher risk of getting COVID-19 compared with those in bus 1 and were 11.4 (95% CI, 5.1-25.4) times more likely to have COVID-19 compared with all other individuals attending the worship event. Within bus 2, individuals in high-risk zones had moderately, but nonsignificantly, higher risk for COVID-19 compared with those in the low-risk zones. The absence of a significantly increased risk in the part of the bus closer to the index case suggested that airborne spread of the virus may at least partially explain the markedly high attack rate observed.
In this cohort study and case investigation of a community outbreak of COVID-19 in Zhejiang province, individuals who rode a bus to a worship event with a patient with COVID-19 had a higher risk of SARS-CoV-2 infection than individuals who rode another bus to the same event. Airborne spread of SARS-CoV-2 seems likely to have contributed to the high attack rate in the exposed bus. Future efforts at prevention and control must consider the potential for airborne spread of the virus.
Photosynthetic organisms have developed various light-harvesting systems to adapt to their environments
. Phycobilisomes are large light-harvesting protein complexes found in cyanobacteria and red ...algae
, although how the energies of the chromophores within these complexes are modulated by their environment is unclear. Here we report the cryo-electron microscopy structure of a 14.7-megadalton phycobilisome with a hemiellipsoidal shape from the red alga Porphyridium purpureum. Within this complex we determine the structures of 706 protein subunits, including 528 phycoerythrin, 72 phycocyanin, 46 allophycocyanin and 60 linker proteins. In addition, 1,598 chromophores are resolved comprising 1,430 phycoerythrobilin, 48 phycourobilin and 120 phycocyanobilin molecules. The markedly improved resolution of our structure compared with that of the phycobilisome of Griffithsia pacifica
enabled us to build an accurate atomic model of the P. purpureum phycobilisome system. The model reveals how the linker proteins affect the microenvironment of the chromophores, and suggests that interactions of the aromatic amino acids of the linker proteins with the chromophores may be a key factor in fine-tuning the energy states of the chromophores to ensure the efficient unidirectional transfer of energy.
Positive psychological factors play a pivotal role in improving cardiovascular outcomes. Gratitude interventions are among the most effective positive psychological interventions, with potential ...clinical applications in cardiology practice. To better understand the potential clinical effects of gratitude interventions in cardiovascular disease, four databases (Web of Science, Scopus, PubMed, and PsycArticles) were searched from 2005 to 2023 for relevant studies. Randomized controlled trials of gratitude interventions as the intervention and that reported physiological or psychosocial outcomes were eligible for inclusion. In total, 19 studies were identified, reporting results from 2951 participants from 19 to 71 years old from both healthy populations and those with clinical diagnoses. The studies showed that gratitude not only promotes mental health and adherence to healthy behaviors but also improves cardiovascular outcomes. Gratitude may have a positive impact on biomarkers of cardiovascular disease risk, especially asymptomatic heart failure, cardiovascular function, and autonomic nervous system activity.
Gastrulation is a key event in embryonic development when the germ layers are specified and the basic animal body plan is established. The complexities of primate gastrulation remain a mystery ...because of the difficulties in accessing primate embryos at this stage. Here, we report the establishment of an in vitro culture (IVC) system that supports the continuous development of cynomolgus monkey blastocysts beyond early gastrulation up to 20 days after fertilization. The IVC embryos highly recapitulated the key events of in vivo early postimplantation development, including segregation of the epiblast and hypoblast, formation of the amniotic and yolk sac cavities, appearance of the primordial germ cells, and establishment of the anterior-posterior axis. Single-cell RNA-sequencing analyses of the IVC embryos provide information about lineage specification during primate early postimplantation development. This system provides a platform with which to explore the characteristics and mechanisms of early postimplantation embryogenesis in primates with possible conservation of cell movements and lineages in human embryogenesis.
Laccases are encoded by a multigene family and widely distributed in plant genomes where they play roles oxidizing monolignols to produce higher-order lignin involved in plant development and stress ...responses. We identified 30 laccase genes (OsLACs) from rice, which can be divided into five subfamilies, mostly expressed during early development of the endosperm, growing roots, and stems. OsLACs can be induced by hormones, salt, drought, and heavy metals stresses. The expression level of OsLAC10 increased 1200-fold after treatment with 20 μM Cu for 12 h. The laccase activities of OsLAC10 were confirmed in an Escherichia coli expression system. Lignin accumulation increased in the roots of Arabidopsis over-expressing OsLAC10 (OsLAC10-OX) compared to wild-type controls. After growth on 1/2 Murashige and Skoog (MS) medium containing toxic levels of Cu for seven days, roots of the OsLAC10-OX lines were significantly longer than those of the wild type. Compared to control plants, the Cu concentration decreased significantly in roots of the OsLAC10-OX line under hydroponic conditions. These results provided insights into the evolutionary expansion and functional divergence of OsLAC family. In addition, OsLAC10 is likely involved in lignin biosynthesis, and reduces the uptake of Cu into roots required for Arabidopsis to develop tolerance to Cu.
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