We discuss the calculation of charged Higgs boson production in association with a top quark in the MC@NLO framework for combining NLO matrix elements with a parton shower. The process is defined in ...a model-independent manner for wide applicability, and can be used if the charged Higgs boson mass is either greater or less than the mass of the top quark. For the latter mass region, care is needed in defining the charged Higgs production mode due to interference with top pair production. We give a suitable definition applicable in an NLO (plus parton shower) context, and we present example results for the LHC.
Pancreatic cancer has low levels of antioxidant enzymes including manganese superoxide dismutase (MnSOD), which converts superoxide radical (O(2)(*-)) into hydrogen peroxide (H(2)O(2)), and ...glutathione peroxidase (GPx), which converts H(2)O(2) into water. Recent studies have demonstrated that overexpression of MnSOD has a tumor-suppressive effect in pancreatic cancer. However, GPx overexpression has been shown to reverse the tumor cell growth inhibition caused by MnSOD overexpression in other types of cancer. Our aims were to determine if overexpression of GPx alters in vitro pancreatic cancer cell behavior and if delivering the GPx gene directly to tumor xenografts alters growth and survival. In vitro, AdGPx slowed tumor growth by 39% and AdMnSOD slowed tumor growth by 35%. AdGPx also decreased plating efficiency and growth in soft agar. The combination of AdGPx and AdMnSOD had the greatest effect on tumor cell growth suppression with a 71% reduction in cell growth compared to controls. In vivo, either AdGPx or AdMnSOD alone slowed tumor growth by 51% and 54%, respectively, while the combination of AdGPx and AdMnSOD potentiated tumor growth suppression by 81% of controls and increased animal survival. GPx may be a tumor suppressor gene in pancreatic cancer. Delivery of the GPx gene alone or in combination with the MnSOD gene may prove beneficial for treatment of pancreatic cancer.
The recent and rapid invasion of Europe and North-America by Drosophila suzukii has generated numerous laboratory and field studies since this fly species is an agricultural pest that causes ...economical losses by laying eggs and developing in ripening fruits, but also because of its relatedness with the model species Drosophila melanogaster whose biology and genetics are well described. This commentary is based on recent data published by different research groups as well as some of our own preliminary results. It discusses the state of research on D. suzukii and addresses the main following questions : can the wide knowledge on D. melanogaster help us to understand and manage the D. suzukii invasion and how ? Are lab results on D. suzukii really informative on its biology ? How can the ecological impact of an invasive species be evaluated if the ecological niche is poorly described in terms of biodiversity and trophic network ? We also outline constraints for the biological control of this pest species and suggest new possible approaches for its long-term management.
L’invasion mondiale de Drosophila suzukii : être une espèce «cousine» d’un organisme-modèle aide-t-il réellement à établir un contrôle biologique ? Espoirs, désillusions et nouvelles perspectives. — L’invasion récente et rapide de Drosophila suzukii en Europe et en Amérique du Nord a suscité de nombreuses études sur le terrain comme en laboratoire. Cette drosophile est en effet à la fois un ravageur important, du fait de son développement dans des fruits à maturité, et une «cousine» de l’espèce modèle Drosophila melanogaster dont la biologie et la génétique sont bien connues. Cet article s’appuie sur les données publiées par différentes équipes et sur des résultats préliminaires pour discuter de l’avancée des recherches et notamment des questions suivantes : en quoi nos connaissances sur D. melanogaster peuvent-elles ou non aider à comprendre et gérer l’invasion de D. suzukii ? Les résultats obtenus en condition de laboratoire avec cette espèce sont-ils représentatifs de sa biologie ? Comment mesurer l’impact écologique d’une espèce invasive si la niche qu’elle vient occuper est peu décrite localement en termes d’espèces présentes et de réseaux trophiques ? Cet article discute aussi des pistes et contraintes en termes de lutte biologique et de biocontrôle.
Iacovone A.,Girod P.,Ris Nicolas,Weydert C.,Gibert P.,Poirié M.,Gatti Jean-Luc. Worldwide invasion by Drosophila suzukii : does being the “cousin” of a model organism really help setting up biological control ? Hopes, disenchantments and new perspectives . In: Revue d'Écologie (La Terre et La Vie), supplément n°12, 2015. Les invasions biologiques et leur gestion au carrefour des sciences naturelles et des sciences humaines et sociales. Colloque organisé à Rennes (Université de Rennes 1) du 20 au 22 octobre 2014. pp. 207-214.
Metal honeycombs are commonly used to absorb energy in applications involving impact because of their high-energy capacity to weight ratio. The energy capacity of a material will be affected by any ...strain-rate effect in the material; thus knowledge of this property is necessary for design purposes. The materials studied in this work were a thick-walled aluminum and stainless-steel honeycomb. A method of testing the specimens in a state approximating uniaxial strain was developed for the large compressive deformations required with honeycombs, and used for both static and dynamic tests. For the dynamic tests, a gas gun was used to propel projectiles into fixed specimens of the test materials, and measurements of the force at the fixed ends of the specimens were used to determine the energy absorption properties of the materials. Initial strain rates to about 2000/s were obtained. Both materials showed a strain-rate effect.
The associated production of charged Higgs bosons and top quarks at hadron colliders is an important discovery channel to establish the existence of a non-minimal Higgs sector. Here, we present ...details of a next-to-leading order (NLO) calculation of this process using the Catani–Seymour dipole formalism and describe its implementation in POWHEG, which allows to match NLO calculations to parton showers. Numerical predictions are presented using the PYTHIA parton shower and are compared to those obtained previously at fixed order, to a leading order calculation matched to the PYTHIA parton shower, and to a different NLO calculation matched to the HERWIG parton shower with MC@NLO. We also present numerical predictions and theoretical uncertainties for various Two Higgs Doublet Models at the Tevatron and LHC.
Activator protein-2 (AP-2) is a transcription factor with transactivating and transrepressing potential in different promoter contexts. AP-2 contains seven cysteines, and its in vitro DNA binding ...activity is redox-sensitive. Superoxide dismutase-2 (SOD2), which encodes the antioxidant enzyme manganese superoxide dismutase (MnSOD), is a putative tumor suppressor gene whose loss of expression is associated with the malignant phenotype. SOD2 promoter mutations that generate new AP-2 sites are associated with loss of MnSOD expression in cancer cells. In the current study, we have identified an inverse expression pattern between AP-2 and MnSOD in normal versus transformed human cells. MRC5 cells are a normal human lung fibroblast cell strain that is mortal and senesces after a certain number of passages in vitro. MRC5-VA is a simian virus transformed variant of MRC5. We determined the levels of expression of MnSOD and AP-2 in these two cell types at the levels of mRNA, protein, and activity. Our results indicated that MnSOD expression was significantly decreased in MRC5-VA cells compared with MRC5 cells at each level of investigation, whereas AP-2 showed an opposing pattern of expression and DNA binding activity. These results suggest that AP-2 may participate in the mechanism(s) underlying decreased expression of SOD2 in transformed cells.
Chronic pancreatitis, K-ras oncogene mutations, and the subsequent generation of reactive oxygen species (ROS) appear to be linked to pancreatic cancer. ROS have also been suggested to be mitogenic ...and capable of stimulating cell proliferation. Cells contain antioxidant enzymes to regulate steady state levels of ROS produced by products of metabolism. The aims of our study were to determine antioxidant enzyme activity in pancreatic cancer cells and correlate enzyme activity with tumor growth, as well as determine whether tumor cell growth could be altered with antioxidant gene transfection. Western blots, enzyme activity, and enzyme activity gels were performed for manganese superoxide dismutase (MnSOD), copper/zinc, catalase, and glutathione peroxidase in normal human pancreas and in the human pancreatic cancer cell lines BxPC-3, Capan-1, MIA PaCa-2, and AsPC-1. Cell population doubling times were determined and correlated with antioxidant enzyme activity. MnSOD was overexpressed in MIA PaCa-2 using an adenoviral vector, and the effect on cell growth was determined. The cell pancreatic cancer lines BxPC-3, MIA PaCa-2, and AsPC-1 had decreased levels of MnSOD immunoreactive protein as well as activity and decreases in MnSOD levels correlated well with increased rates of tumor cell proliferation as determined by cell doubling time. No correlation could be found between cell growth and levels of copper/zinc superoxide dismutase, catalase, or glutathione peroxidase. Enforced expression of MnSOD by adenovirus transfection in the rapid growing cell line MIA PaCa-2 increased MnSOD immunoreactivity and MnSOD activity and decreased growth rate. Overexpression of MnSOD may be effective in growth suppression of pancreatic cancer.
This study investigates the role of the antioxidant enzyme manganese superoxide dismutase (MnSOD) in androgen-independent human prostate cancer (PC-3) cells' growth rate in vitro and in vivo. MnSOD ...levels were found to be lower in parental PC-3 cells compared to nonmalignant, immortalized human prostate epithelial cells (P69SV40T). To unravel the role of MnSOD in the prostate cancer phenotype, PC-3 cells were stably transfected with MnSOD cDNA plasmid. The MnSOD protein and activity levels in clones overexpressing MnSOD were increased seven- to eightfold. These cell lines showed elongated cell doubling time, reduced anchorage-independent growth in soft agar compared to parental PC-3 (Wt) cells, and reduced growth rate of PC-3 tumor xenografts in athymic nude mice. Flow cytometric studies showed an increase in membrane potential in the MnSOD-overexpressing clone (Mn32) compared to Wt and Neo cells. Also, production of extracellular H(2)O(2) was increased in the MnSOD-overexpressing clones. As determined by DNA cell cycle analysis, the proportion of cells in G(1) phase was enhanced by MnSOD overexpression. Therefore, MnSOD not only regulates cell survival but also affects PC-3 cell proliferation by retarding G(1) to S transition. Our results are consistent with MnSOD being a tumor suppressor gene in human prostate cancer.
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