We present new constraints on sub-GeV dark-matter particles scattering off electrons based on 6780.0 kg d of data collected with the DarkSide-50 dual-phase argon time projection chamber. This ...analysis uses electroluminescence signals due to ionized electrons extracted from the liquid argon target. The detector has a very high trigger probability for these signals, allowing for an analysis threshold of three extracted electrons, or approximately 0.05 keVee. We calculate the expected recoil spectra for dark matter-electron scattering in argon and, under the assumption of momentum-independent scattering, improve upon existing limits from XENON10 for dark-matter particles with masses between 30 and 100 MeV/c^{2}.
A very good knowledge of material properties is required in the analysis of severe plastic deformation problems in which the classical material processing methods are accelerated by the application ...of the additional cyclic load. A general fuzzy logic-based approach is proposed for the analysis of experimental and numerical data in this paper. As an application of the fuzzy analysis, the calibration of Chaboche–Lemaitre model hardening parameters of PA6 aluminum is considered here. The experimental data obtained in a symmetrical strain-controlled cyclic tension–compression test were used to estimate the material’s hardening parameters. The numerically generated curves were compared to the experimental ones. For better fitting of numerical and experimental results, the optimization approach using the least-square method was applied. Unfortunately, commonly accepted calibration methods can provide various sets of hardening parameters. In order to choose the most reliable set, the fuzzy analysis was used. Primarily selected values of hardening parameters were assumed to be fuzzy input parameters. The error of the hysteresis loop approximation for each set was used to compute its membership function. The discrete value of this error was obtained in the defuzzification step. The correct selections of hardening parameters were verified in ratcheting and mean stress relaxation tests. The application of the fuzzy analysis has improved the convergence between experimental and numerical stress–strain curves. The fuzzy logic allows analyzing the variation of elastic–plastic material response when some imprecisions or uncertainties of input parameters are taken into consideration.
Abstract
Recombinant human erythropoietin (rhEPO) has potent procognitive effects, likely hematopoiesis-independent, but underlying mechanisms and physiological role of brain-expressed EPO remained ...obscure. Here, we provide transcriptional hippocampal profiling of male mice treated with rhEPO. Based on ~108,000 single nuclei, we unmask multiple pyramidal lineages with their comprehensive molecular signatures. By temporal profiling and gene regulatory analysis, we build developmental trajectory of CA1 pyramidal neurons derived from multiple predecessor lineages and elucidate gene regulatory networks underlying their fate determination. With EPO as ‘tool’, we discover populations of newly differentiating pyramidal neurons, overpopulating to ~200% upon rhEPO with upregulation of genes crucial for neurodifferentiation, dendrite growth, synaptogenesis, memory formation, and cognition. Using a Cre-based approach to visually distinguish pre-existing from newly formed pyramidal neurons for patch-clamp recordings, we learn that rhEPO treatment differentially affects excitatory and inhibitory inputs. Our findings provide mechanistic insight into how EPO modulates neuronal functions and networks.
Reactive oxygen species (ROS) generated in the presence of O(2) by mitochondria, phagocytic cells, peroxisomes, and cytochrome P450 enzymes under physiological conditions, may play a dual function in ...the human organism. On the one hand, they participate in cell signal transduction cascades, leading to the activation of some transcription factors responsible for regulating of the expression of genes relevant for cell growth and differentiation. On the other hand, they cause oxidative damage of cellular DNA, protein and lipids, resulting in the initiation or development of numerous diseases such as cancer, cardiovascular diseases, type 2 diabetes mellitus, cataract, rheumatoid arthritis, or different neurodegenerative diseases. Both endogenous compounds (glutathione, ubiquinol, urate, bilirubin) and enzymes (superoxide dismutase, catalase, glutathione peroxidase) are engaged in the detoxification of ROS. In addition, numerous dietary components such as vitamin C, vitamin E, carotenoids, and polyphenols are thought to be involved in the antioxidant defense system. The present review article is focused on the summary and the assessment of research on the impact of dietary antioxidants in the prevention of chronic diseases, particularly cancer and cardiovascular diseases.
•Pool boiling analyzed for micro-fins with perforated foil (MF+F) and mesh (MF+M).•Results compared with plain micro-fins (MF).•The best boiling heat transfer enhancement obtained from MF+M with ...water.•The most effective heat transfer for ethanol, FC-72 and Novec-649 obtained from MF.•Simplified model to predict heat flux with a ±30–40% error.
The paper focuses on the comparison of pool boiling heat transfer on micro-fin enhanced structures with and without porous covering. The purpose of the present study was to find the optimal parameters of the perforated foil or wire mesh sintered to the micro-fin tips to provide the highest heat transfer coefficient. Additionally, a simplified model was proposed for determining total heat flux for investigated surfaces.
Three types of structured surface were used: 1.0 mm high plain micro-fins (designated as MF), enhanced surfaces made by sintering micro-fin tips with the copper perforated foil (MF+F) and structured surfaces formed by sintering the woven copper wire mesh to the micro-fin tips (MF+M).
The experiments were carried out for water, ethanol, Novec-649 and FC-72 at atmospheric pressure. At medium and high heat fluxes for water, low and medium heat fluxes for ethanol and medium heat fluxes for FC-72, surfaces with micro-fins covered with wire mesh produced the highest heat transfer coefficient. The plain micro-fin surfaces helped obtain the increase in the heat transfer coefficient value about two times higher than that of the micro-fins with perforated foil at FC-72 boiling for high heat fluxes. The simplified semi-analytical model was proposed for determining total heat flux for the studied surfaces. The model provided a heat flux prediction with a ±30–40% margin of error.
Vesicular glutamate transporters (VGLUTs) are essential for filling synaptic vesicles with glutamate and mammals express three VGLUT isoforms (VGLUT1–3) with distinct spatiotemporal expression ...patterns. Here, we find that neurons expressing VGLUT1 have lower release probability and less short-term depression than neurons expressing VGLUT2 or VGLUT3. Investigation of the underlying mechanism identified endophilin A1 as a positive regulator of exocytosis whose expression levels are positively correlated with release efficiency and showed that the differences in release efficiency between VGLUT1- and VGLUT2-expressing neurons are due to VGLUT1's ability to bind endophilin A1 and inhibit endophilin-induced enhancement of release probability.
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► Neurons expressing VGLUT1 have lower release probability than neurons with VGLUT2/3 ► VGLUT3 expression is sufficient for glutamate release in GABAergic striatal neurons ► Endophilin A1 expression levels positively correlate with release probability ► VGLUT1 binding to endophilin inhibits endophilin's enhancement of exocytosis
Liquid argon is a bright scintillator with potent particle identification properties, making it an attractive target for direct-detection dark matter searches. The DarkSide-50 dark matter search here ...reports the first WIMP search results obtained using a target of low-radioactivity argon. DarkSide-50 is a dark matter detector, using a two-phase liquid argon time projection chamber, located at the Laboratori Nazionali del Gran Sasso. The underground argon is shown to contain super(39) Ar at a level reduced by a factor (1.4+ or -0.2)x10 super(3) relative to atmospheric argon. We report a background-free null result from (2616+ or -43)kgd of data, accumulated over 70.9 live days. When combined with our previous search using an atmospheric argon, the 90% C.L. upper limit on the WIMP-nucleon spin-independent cross section, based on zero events found in the WIMP search regions, is 2.0x10 super(-44)cm super(2)(8.6x10-44cm super(2), 8.0x10 super(-43)cm super(2)) for a WIMP mass of 100GeV/c super(2)(1TeV/c super(2), 10TeV/c super(2)).
Unlike most other secretory processes, neurotransmitter release at chemical synapses is extremely fast, tightly regulated, spatially restricted, and dynamically adjustable at the same time. In this ...review, we focus on recent discoveries of molecular and cell biological processes that determine how fusion competence of vesicles is achieved and controlled in order to suit the specific requirements of synaptic transmitter release with respect to speed and spatial selectivity.
The aim of this study was to investigate the potential impact of guided imagery (GI) on attentional control and cognitive performance and to explore the relationship between guided imagery, stress ...reduction, alpha brainwave activity, and attentional control using common cognitive performance tests. Executive function was assessed through the use of attentional control tests, including the anti-saccade, Stroop, and Go/No-go tasks. Participants underwent a guided imagery session while their brainwave activity was measured, followed by attentional control tests. The study's outcomes provide fresh insights into the influence of guided imagery on brain wave activity, particularly in terms of attentional control. The findings suggest that guided imagery has the potential to enhance attentional control by augmenting the alpha power and reducing stress levels. Given the limited existing research on the specific impact of guided imagery on attention control, the study's findings carry notable significance.
The main purpose of urine cytology is to detect high-grade urothelial carcinoma (HGUC). With this principle in mind, The Paris System (TPS) Working Group, composed of cytopathologists, surgical ...pathologists, and urologists, has proposed and published a standardized reporting system that includes specific diagnostic categories and cytomorphologic criteria for the reliable diagnosis of HGUC. This paper outlines the essential elements of TPS and the process that led to the formation and rationale of the reporting system. The Paris System Working Group, organized at the 2013 International Congress of Cytology, conceived a standardized platform on which to base cytologic interpretation of urine samples. The widespread dissemination of this approach to cytologic examination and reporting of urologic samples and the scheme's universal acceptance by pathologists and urologists is critical for its success. For urologists, understanding the diagnostic criteria, their clinical implications, and the limitations of TPS is essential if they are to utilize urine cytology and noninvasive ancillary tests in a thoughtful and practical manner. This is the first international/inclusive attempt at standardizing urinary cytology. The success of TPS will depend on the pathology and urology communities working collectively to improve this seminal paradigm shift, and optimize the impact on patient care.
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