Six ruminally cannulated steers (average BW = 791 ± 71 kg) were used in a replicated 3 × 3 Latin square experiment to determine the effects of roughage type on rumination, fiber mat characteristics, ...and rumen fermentation variables. Three roughages were included at 7% (DM basis) in a steam flaked corn-based diet: cotton burrs (CB), wheat silage (WS), or corn stalks (CS). Steers were fitted with a sensory collar to record rumination behaviors in 2-h intervals at the beginning of the experiment. Each 30-d period consisted of 7 d of recovery, 14 d of diet adaptation, 7 d of rumination data collection (daily and bi-hourly average rumination), 1 d of rumen fluid collection, and 1 d of rumen evacuations. In situ degradation of individual roughages was determined for 4 d after period 3 evacuations. During rumen evacuations, ruminal contents were removed; the rumen fiber mat (RF) was separated from the liquid portion with a 2-mm sieve, weighed, and a subsample was dried. Data were analyzed using the MIXED procedure of SAS with steer as the experimental unit and roughage (CB, WS, and CS) as the main effect. Dry matter intake (DMI) was not different for CB and WS (P = 0.25) and greatest for steers consuming CS diet (P ≤ 0.01). Roughage type did not influence the weight of the RF dry matter (%; DM; P = 0.92), RF weight (P = 0.69), or RF:DMI ratio (P = 0.29). Daily rumination (min/d) did not differ among roughages (P = 0.40), but min of rumination/kg of DMI was greatest for CS (18.0 min), min/kg of NDF was greatest for WS (89.8 min; P = 0.02), and min/kg of peNDF was greatest for CS (132.4 min; P ≤ 0.01). Wheat silage had the greatest percentage of soluble and degradable DM. Rumen fiber mat did not differ for roughages, although rumination min/kg of DMI and peNDF was greatest for steers consuming CS and WS. In situ degradation determined that CB-R and CS-R had the greatest percentage of ruminal undegraded DM. Based on the objective of the experiment, roughage type did not influence daily rumination or fiber mat characteristics.
Abstract
The objective of this study was to determine the efficacy of the direct-fed microbial 10-G upon cattle growth performance, liver and lung health, carcass quality and yield outcomes, as well ...as prevalence and enumeration of Salmonella in feces and lymph nodes. Fed beef heifers (N = 1,400; initial shrunk body weight BW 343.3 ± 36.2 kg) were blocked by day of arrival and randomly allocated to one of two treatments 0 (negative control, CON) or 2 g of a direct fed microbial (10-G) that provided 1 billion CFUs per animal per day of Lactobacillus acidophilus, Enterococcus faecium, Pediococcus pentosaceus, Lactobacillus brevis and Lactobacillus plantarum with ten pens per treatment. Recto-anal mucosal fecal samples (RAMs; n = 477) and subiliac lymph nodes (SLNs; n = 479) were collected longitudinally at harvest from 23 to 25 heifers per pen. Data were analyzed using mixed models; pen served as the experimental unit; block and harvest date were random effects. No differences were detected in dry matter intake (P = 0.78), final BW (P = 0.64), average daily gain (P = 0.51), gain to feed (P = 0.71), hot carcass weight (P = 0.54), dressed carcass yield (P = 0.52), 12th rib fat depth (P = 0.13), longissimus muscle area (P = 0.62), calculated empty body fat (P = 0.26), or marbling score (P = 0.82). Distributions of liver scores (P ≥ 0.34), quality grades (P ≥ 0.23), and yield grades (P ≥ 0.11) were also not different between treatments. A tendency was detected for more normal lungs (P = 0.08; 10-G = 65.96%, CON = 61.12%) and fewer inflated lungs at harvest for cattle fed 10-G (P = 0.10; 10-G = 0.29%, CON = 1.16%); other lung outcomes did not differ (P ≥ 0.54). Salmonella prevalence did not differ for RAM samples (P = 0.41; 10-G = 97.74%, CON = 96.82%) or SLN (P = 0.22; 10-G = 17.92%, CON = 13.66%). Salmonella concentration of RAM samples (P = 0.25; 10-G = 3.87 log CFU/g, CON = 3.32 log CFU/g) or SLN (P = 0.37; 10-G = 1.46 log CFU/g, CON = 1.14 log CFU/g) also did not differ between treatments at harvest. These results do not demonstrate any difference in live animal performance, carcass characteristics or Salmonella carriage for heifers fed 10-G.
Abstract
Crossbred beef heifers (N = 1,394; initial shrunk body weight BW 291 ± 9.9 kg) were used to investigate the efficacy of 10-G Armor (Life Products, Inc., Norfolk, NE; 10-G) upon feedlot ...performance, carcass characteristics, and fecal and subiliac lymph nodes Salmonella prevalence. Heifers were blocked by day of arrival and allocated to 1 of 20 pens (N = 70 heifers/pen) and assigned one of two treatments (10 pens/treatment): no direct-fed microbial (CON) or 2 g/heifer/d of Lactobacillus acidophilus, Enterococcus faecium, Pediococcus pentosaceus, Lactobacillus brevis and Lactobacillus plantarum, respectively (Life Products, Inc., Norfolk, NE; 10-G). Twenty-four animals were randomly selected from each pen for Salmonella sampling. Recto-anal mucosal swab samples (RAMS) were obtained at initial processing and harvest; subiliac lymph nodes were collected at harvest. In addition, pen surface fecal pats were collected and composited by pen (10 pats per composite, 5 composites per pen) on days 0, 52, 120, and 192. Data were analyzed as a generalized complete block design, and pen served as the experimental unit. No differences were observed in live growth performance metrics (P ≥ 0.55). Yield grade distributions did not differ between treatments (P ≥ 0.62); however, cattle fed 10-G tended (P = 0.06; 14.6% vs. 18.9%) to have fewer USDA Select carcasses and more (P = 0.09; 73.6% vs. 78.0%) USDA Choice carcasses. Cattle fed 10-G tended (P = 0.10; 9.2% vs. 12.3%) to have fewer liver abscesses and had fewer (P = 0.04; 5.3% vs. 8.5%) severe liver abscesses. Salmonella prevalence of RAMS did not differ between treatments at initial processing (P = 0.97; CON = 11.6%, 10-G = 11.5%) or at harvest (P = 0.91; CON = 99.0%, 10-G = 98.6%); however, RAMS differed (P < 0.01) in Salmonella prevalence between the two collection times. Cattle fed 10-G had a lower frequency of Salmonella positive lymph nodes (P = 0.01; CON = 15.8%, 10-G = 7.4%) than CON. However, Salmonella log (mpn/g) of lymph nodes did not differ between treatments at harvest (P = 0.34; CON = 0.73, 10-G = 0.34). These data indicate that cattle fed 10-G have decreased rates of severe liver abscesses without altering live animal performance or carcass characteristics. Supplementation of 10-G significantly reduced the prevalence rate of Salmonella recovered from the subiliac lymph nodes. The factors responsible for the observed difference in the effects of 10-G on Salmonella warrant further investigation
Abstract
Lubabegron (LUB; Experior, Elanco, Greenfield, IN, USA) was approved by the U.S. Food and Drug Administration in 2018 and is indicated for the reduction of ammonia (NH3) gas emissions·kg−1 ...body weight (BW) and hot carcass weight (HCW) when fed to feedlot cattle during the final 14 to 91 d of the finishing period. LUB demonstrates antagonistic behavior at the β 1 and β 2 receptor subtypes and agonistic behavior at the β 3 receptor subtype in cattle and is classified by the Center for Veterinary Medicine as a “beta-adrenergic agonist/antagonist.” This report describes a randomized complete block study that evaluated LUB dose (0, 1.5, 3.5, and 5.5 mg·kg−1 dry matter) during the last 56 d of the feeding period on calculated NH3 gas emissions, live weight, carcass weight, and associated ratios in beef feedlot cattle. Carcass characteristics, mobility, and health were also evaluated. All cattle received monensin and tylosin throughout the study. Ammonia gas emissions were calculated using the equation developed by Brown et al. (Brown, M. S., N. A. Cole, S. Gruber, J. Kube, and J. S. Teeter. 2019. Modeling and prediction accuracy of ammonia gas emissions from feedlot cattle. App. Anim. Sci. 35:347–356). The reduction in calculated cumulative NH3 gas emissions with LUB ranged from 1.3% to 11.0% (85 to 708 g/hd). When NH3 gas emissions were expressed on a live weight (unshrunk) and carcass weight basis, calculated NH3 gas emissions decreased by 3.0% to 12.8% and 3.8% to 14.6%, respectively. Daily dry matter intake was 2.3% greater (Ptrt < 0.05) for steers that received LUB. Average daily gain was 13.7% greater (Ptrt < 0.05; 1.68 vs. 1.91 kg), while gain efficiency was 10.8% greater (Ptrt < 0.05; 0.167 vs. 0.185) for steers fed LUB. Animal mobility was scored in the pen approximately 1 wk prior to harvest, when cattle were loaded on trucks scheduled for harvest, and at antemortem inspection during lairage. No treatment differences (Ptrt ≥ 0.170) were observed at any time for the percent of cattle receiving mobility scores of 1 or 2 (normal or minor stiffness but moving with the normal cattle, respectively). Cattle mobility scored as a 1 or 2 equaled or exceeded 92% at all times. Final BW and HCW increased (Ptrt < 0.05) 11.6 to 15.7 kg and 11.3 to 17.1 kg, respectively, in cattle receiving LUB compared to cattle receiving monensin plus tylosin alone.
Abstract
Research objectives were to evaluate effects of two implant programs for beef heifers fed three different durations (days-on-feed; DOF) on carcass weight and composition (primary outcomes) ...and feedlot performance (secondary outcomes) at commercial feedlots. Data from a randomized trial in Kansas were analyzed separately and also pooled with data from two previously published trials conducted in Texas. Heifers were randomly allocated to pens within a block, and pens were randomized to treatments in a 2 × 3 factorial randomized complete block design. Implant programs were IH + 200 – an initial Revalor-IH implant 80 mg trenbolone acetate (TBA) and 8 mg estradiol (E2) and a re-implant after a mean of 98-d (± 10.8 SD) with Revalor-200 (200 mg TBA and 20 mg E2), or XH – Revalor-XH, a single extended-release implant (200 mg TBA and 20 mg E2). Heifers were fed to a baseline endpoint (BASE; pooled mean 166-d ± 11.9 SD), +21, or +42 additional DOF. A total of 10,583 crossbred heifers with mean initial body weight (BW) 315 kg (± 20.1 SD) were enrolled in 144 pens in 24 blocks (treatment replications) across the three trials. General and generalized linear mixed models accounting for clustering of trials, blocks, and pens were used to test for effects of treatments, with significance set at α = 0.05. The only implant program × DOF interaction in pooled analyses was for dry matter intake (DMI; P < 0.01); IH + 200 heifers had lower mean DMI than XH when fed +42 DOF. Gain:feed was higher for IH + 200 compared to XH with dead and removed animals excluded (P < 0.01) or included (P = 0.03). For IH + 200, hot carcass weight (HCW) increased (P < 0.01), USDA Yield Grade (YG) distributions shifted towards lower numerical categories (P < 0.01), and Prime carcasses decreased while Select increased compared to XH (P < 0.01). For each incremental increase in DOF, final BW (P < 0.01) and HCW increased (P < 0.01), while daily gain (P < 0.01) and gain:feed (P < 0.01) decreased. Categories of YG were affected by DOF (P < 0.01); there were fewer YG 1 and 2 and more YG 4 and 5 carcasses for +42 compared to BASE and +21. USDA Quality Grade (QG) distributions differed by DOF (P < 0.01); each incremental increase in DOF resulted in more Prime and fewer Select carcasses. Without meaningful interactions, tested implant programs likely have a consistent effect when heifers are fed to similar DOF, while changes in HCW, QG, and YG may influence marketing decisions when extending DOF.
Abstract
Two experiments evaluated the effect of implant number, type, and total steroidal dose on live animal performance and carcass traits in heifers fed for three different days on feed (DOF). In ...experiment 1, heifers (n = 3,780; 70 heifers/pen and 9 pens/treatment; initial body weight BW = 309 kg) were used in a 2 × 3 factorial arrangement of treatments. Factors were as follows: 1) implant (all from Merck Animal Health, De Soto, KS): 200 mg trenbolone acetate (TBA) and 20 mg estradiol-17β (E2) administered on arrival (SINGLE), or 80 mg TBA and 8 mg E2 administered on arrival followed by 200 mg TBA and 20 mg E2 after approximately 90 d (REPEATED) and 2) duration of DOF: harvested after approximately 172, 193, and 214. In experiment 2, heifers (n = 3,719; 65 to 70 heifers/pen and 9 pens/treatment; initial BW = 337 kg) were used with the same factors as experiment 1, except DOF were 150, 171, and 192. No implant × DOF interaction (P ≥ 0.06) was noted for any performance parameters in either experiment. Heifers administered REPEATED had improved (P ≤ 0.05) live gain to feed ratio (G:F) and carcass-adjusted G:F and tended (P = 0.09) to have greater hot carcass weight (HCW) in experiment 1. Increasing DOF resulted in greater (P ≤ 0.01) live and carcass-adjusted final BW and decreased (P = 0.01) live ADG in experiment 1. As DOF increased, HCW, HCW gain, and dressing% (P ≤ 0.01) increased in experiment 1. The mean carcass transfer was 79.6% across the 42 d terminal window in experiment 1. In experiment 2, REPEATED had improved (P = 0.03) carcass-adjusted G:F compared with SINGLE, but HCW was not different (P = 0.36) between treatments. Increased DOF resulted in greater (P ≤ 0.01) final live and carcass-adjusted BW, decreased (P ≤ 0.01) live and carcass-adjusted ADG, and poorer (P ≤ 0.01) live and carcass-adjusted G:F in experiment 2. In experiment 2, dressing percentage was greater (P = 0.02) in REPEATED compared with SINGLE. Heifers given SINGLE had greater (P = 0.01) back fat and estimated empty body fat (EBF), whereas REPEATED had fewer (P = 0.01) Yield Grade 4 carcasses and greater (P = 0.01) longissimus muscle (LM) area. Increased DOF resulted in greater (P ≤ 0.04) HCW, HCW gain, dressing%, back fat, LM area, marbling, EBF%, and United States Department of Agriculture (USDA) Prime-grading carcasses, Yield Grade 4 and 5, and over 454-kg carcasses in experiment 2. Carcass ADG and carcass transfer indicate a 0.70 kg carcass ADG between 150 and 192 DOF, resulting in an average carcass transfer of 72.2% in experiment 2. Although feedlot growth performance and HCW did not differ between the implant regimens tested, increasing DOF resulted in decreased live growth performance while increasing the proportion of USDA prime carcasses and HCW.
Feedlot efficiency increases as technologies are adopted and new feed ingredients, especially byproducts, become available and incorporated into diets. Byproduct availability increased in response to ...the renewable fuels standard of 2005, creating substantial amounts of feedstuffs best used by ruminants. Cereal grains have been partially replaced with human-inedible byproducts, as they provide comparable levels of energy in cattle diets. To evaluate the effects of changes in diet and feedlot production practices on net protein contribution (NPC) and human-edible protein conversion efficiency (HePCE) across time, a deterministic NPC model was used. NPC was assessed for the feedlot industry using lot level production data from 2006 to 2017 for eight commercial feedlots. Ingredient and nutrient composition was collected for a representative starter and finisher diet fed for each year from each feedlot. NPC was calculated by multiplying human-edible protein (HeP) in beef produced per unit of HeP in feed by the protein quality ratio (PQR). Systems with NPC >1 positively contribute to meeting human protein requirements; NPC < 1 indicates competition with humans for HeP. NPC was regressed on year to evaluate temporal change in NPC. Feedlots were categorized as increasing NPC (INC; slope > 0) or constant NPC (CON; slope = 0) according to regression parameter estimates. Four feedlots were categorized as INC and four were CON. The rate of change in PQR was similar for CON and INC (P ≥ 0.79), although rates of change among INC and CON differed for byproduct and cereal grain inclusion (P ≤ 0.01) across years evaluated. Feedlots categorized as INC reduced HeP consumed by 2.39% per year, but CON feedlots did not reduce HeP consumed each year (0.28%). Cattle received and shipped by INC were lighter than those in CON feedlots (P < 0.01). Across years, INC produced more HeP (20.9 vs. 19.2 kg/hd) than CON (P < 0.01), and both feedlot types tended to improve HeP gained over time (0.1 kg per year; P = 0.10). Differences in slope over time for INC and CON were observed for conversion efficiency of HeP (P < 0.01). NPC increased 0.027 units per year for INC (P < 0.01) and was 0.94 in 2017. NPC by the feedlot sector improved from 2006 to 2017, decreasing the amount of human-edible feeds required to produce more high-quality protein from beef.
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