•We successfully generated a ovarian cells model with excessive autophagy, which provided a research tool for studying POI in vitro.•MT protected against both the decline of AMH expression and ...mitochondrial membrane potential induced by excessive autophagy.•MT protected against excessive autophagy-induced decrease of nucleus-encoded proteins and mitochondrial dynamic-related proteins.•MT decreased mitochondrial oxidative stress, increased SOD2 and ameliorated the G2/M cell cycle arrest induced by excessive autophagy.•MT inhibited excessive autophagy-induced activation of ERK signaling pathway.
Excessive autophagy-induced follicular atresia of ovarian granulosa cells might be one of the pathogenesis of Premature Ovarian Insufficiency (POI), and melatonin (MT) exerted many beneficial effects on mitochondria. However, there was little report regarding the beneficial effects of MT on excessive autophagy-induced mitochondrial and ovarian reserve function deficiency, and the mechanisms have not been clearly identified. Autophagy played a protective role in cells survival, however, high level of autophagy could lead to cell death. In this report, firstly, Chinese hamster ovary cell damage model stably expressing EGFP-LC3 was established. Next, we systematically investigated the protective effects of MT on mitochondrial and ovarian reserve function and molecular mechanisms using this cell damage model. Our results revealed that 10-9 M MT not only protected against the decline of anti-mullerian hormone (AMH) expression induced by excessive autophagy, but also rescued excessive autophagy-induced impairment of mitochondrial expression and mitochondrial membrane potential. Furthermore, MT protected against excessive autophagy-induced decrease of nucleus-encoded proteins including SDHA and mitofilin, and mitochondrial dynamic-related proteins including OPA1, MFN2, and DRP1. MT also decreased mitochondrial oxidative stress, increased antioxidant enzyme superoxide dismutase 2 (SOD2) expression and ameliorated the G2/M cell cycle arrest induced by excessive autophagy. Finally, MT inhibited excessive autophagy-induced activation of extracellular signal regulated kinase (ERK) signaling pathway. In conclusion, our study showed that MT rescued impairment of mitochondrial and ovarian reserve function, and production of mitochondrial ROS and cell cycle arrest induced by excessive autophagy through down-regulated ERK pathway, implying the potential therapeutic drug target for POI.
A
bstract
Using (27
.
12 ± 0
.
14) × 10
8
ψ
(3686) events collected by the BESIII detector at the center-of-mass energy of
s
= 3
.
686 GeV, we search for the first time for two nonleptonic hyperon ...decays that change strangeness by two units, Ω
−
→ Σ
0
π
−
and Ω
−
→
nK
−
. No significant signal is observed. The upper limits on their decay branching fractions are determined to be
B
(Ω
−
→ Σ
0
π
−
)
<
5
.
4 × 10
−
4
and
B
(Ω
−
→
nK
−
)
<
2
.
4 × 10
−
4
at the 90% confidence level.
A
bstract
With data samples collected with the BESIII detector at seven energy points at
s
= 3
.
68
−
3
.
71 GeV, corresponding to an integrated luminosity of 333 pb
−
1
, we present a study of the Λ ...transverse polarization in the
e
+
e
−
→
Λ
Λ
¯
reaction. The significance of polarization by combining the seven energy points is found to be 2.6
σ
including the systematic uncertainty, which implies a non-zero phase between the transition amplitudes of the
Λ
Λ
¯
helicity states. The modulus ratio and the relative phase of EM-
psionic
form factors combined with all energy points are measured to be
R
Ψ
=
0.71
−
0.10
+
0.10
±
0.03 and ∆Φ
Ψ
=
23
−
8.0
+
8.8
±
1.6
°
, where the first uncertainties are statistical and the second systematic.
Observation of ψ(3686) →Ω−K+Ξ¯0 + c.c Ai, X. C.; Bakina, O.; Balossino, I. ...
The journal of high energy physics,
04/2024, Letnik:
2024, Številka:
4
Journal Article
Recenzirano
Odprti dostop
A
bstract
Using (27
.
12
±
0
.
14)
×
10
8
ψ
(3686) events collected with the BESIII detector at BEPCII, the decay of
ψ
(3686)
→
Ω
−
K
+
Ξ
¯
0
+ c
.
c
.
is observed for the first time. The branching ...fraction of this decay is measured to be
B
ψ
3686
→
Ω
−
K
+
Ξ
¯
0
+
c
.
c
.
= (2
.
78 ± 0
.
40 ± 0
.
18) × 10
−
6
, where the first uncertainty is statistical and the second is systematic. Possible baryon excited states are searched for in this decay, but no evident intermediate state is observed with the current sample size.
A
bstract
Using 6
.
32 fb
−
1
of
e
+
e
−
collision data collected at the center-of-mass energies between 4.178 and 4.226 GeV with the BESIII detector, we perform an amplitude analysis of the decay
D
...s
+
→ K
+
π
+
π
−
and determine the amplitudes of the various intermediate states. The absolute branching fraction of
D
s
+
→ K
+
π
+
π
−
is measured to be (6
.
11
±
0
.
18
stat
.
±
0
.
11
syst
.
)
×
10
−
3
. The branching fractions of the dominant intermediate processes
D
s
+
→ K
+
ρ
0
, ρ
0
→ π
+
π
−
and
D
s
+
→ K
*
(892)
0
π
+
, K
*
(892)
0
→ K
+
π
−
are determined to be (1
.
96
±
0
.
19
stat
.
±
0
.
23
syst
.
)
×
10
−
3
and (1
.
85
±
0
.
12
stat
.
±
0
.
13
syst
.
)
×
10
−
3
, respectively. The intermediate resonances
f
0
(500),
f
0
(980), and
f
0
(1370) are observed for the first time in this channel.
A
bstract
Using (10087 ± 44) × 10
6
J
/
ψ
events collected with the BESIII detector at the BEPCII
e
+
e
−
storage ring at the center-of-mass energy of
s
= 3.097 GeV, we present a search for the rare ...semi-muonic charmonium decay
J
/
ψ
→
D
−
μ
+
ν
μ
+ c.c.. Since no significant signal is observed, we set an upper limit of the branching fraction to be
B
(
J
/
ψ
→
D
−
μ
+
ν
μ
+ c.c.) < 5.6 × 10
−
7
at 90% confidence level. This is the first search for the weak decay of charmonium with a muon in the final state.
A
bstract
Using a sample of (10
.
09
±
0
.
04)
×
10
9
J/ψ
decays collected with the BESIII detector, partial wave analyses of the decay
J
/
ψ
→
γ
K
S
0
K
S
0
π
0
are performed within the
K
S
0
K
S
0
...π
0
invariant mass region below 1.6 GeV/
c
2
. The covariant tensor amplitude method is used in both mass independent and mass dependent approaches. Both analysis approaches exhibit dominant pseudoscalar and axial vector components, and show good consistency for the other individual components. Furthermore, the mass dependent analysis reveals that the
K
S
0
K
S
0
π
0
invariant mass spectrum for the pseudoscalar component can be well described with two isoscalar resonant states using relativistic Breit-Wigner model, i.e., the
η
(1405) with a mass of
1391.7
±
0.7
−
0.3
+
11.3
MeV/
c
2
and a width of
60.8
±
1.2
−
12.0
+
5.5
MeV, and the
η
(1475) with a mass of
1507.6
±
1.6
−
32.2
+
15.5
MeV/
c
2
and a width of
115.8
±
2.4
−
10.9
+
14.8
MeV. The first and second uncertainties are statistical and systematic, respectively. Alternate models for the pseudoscalar component are also tested, but the description of the
K
S
0
K
S
0
π
0
invariant mass spectrum deteriorates significantly.
A
bstract
Based on
e
+
e
−
collision data collected at center-of-mass energies from 2.000 to 3.080 GeV by the BESIII detector at the BEPCII collider, a partial wave analysis is performed for the ...process
e
+
e
−
→
K
S
0
K
L
0
π
0
. The results allow the Born cross sections of the process
e
+
e
−
→
K
S
0
K
L
0
π
0
, as well as its subprocesses
e
+
e
−
→
K
∗
(892)
0
K
¯
0
and
K
2
∗
(1430)
0
K
¯
0
to be measured. The Born cross sections for
e
+
e
−
→
K
S
0
K
L
0
π
0
are consistent with previous measurements by BaBar, but with substantially improved precision. The Born cross section lineshape of the process
e
+
e
−
K
∗
(892)
0
K
¯
0
is consistent with a vector meson state around 2.2 GeV with a significance of 3.2
σ
. A Breit-Wigner fit determines its mass as
M
Y
= (2164
.
7
±
9
.
1
±
3
.
1) MeV
/c
2
and its width as Γ
Y
= (32
.
4
±
21
.
0
±
1
.
8) MeV.
A
bstract
Using 2.93 fb
−
1
of
e
+
e
−
collision data collected with the BESIII detector at the center-of-mass energy 3.773 GeV, we perform the first amplitude analysis of the decay
D
+
→
K
S
0
π
+
π
...0
π
0
and determine the relative magnitudes and phases of different intermediate processes. The absolute branching fraction of
D
+
→
K
S
0
π
+
π
0
π
0
is measured to be (2.888
±
0.058
stat
.
±
0.069
syst
.
)%. The dominant intermediate processes are
D
+
→
K
S
0
a
1
(1260)
+
(
→ ρ
+
π
0
) and
D
+
→
K
¯
*0
ρ
+
, with branching fractions of (8.66
±
1.04
stat
.
±
1.39
syst
.
)
×
10
−
3
and (9.70
±
0.81
stat
.
±
0.53
syst
.
)
×
10
−
3
, respectively.
The decay
D
→
K
-
π
+
is studied in a sample of quantum-correlated
D
D
¯
pairs, based on a data set corresponding to an integrated luminosity of 2.93 fb
-
1
collected at the
ψ
(
3770
)
resonance by ...the BESIII experiment. The asymmetry between
C
P
-odd and
C
P
-even eigenstate decays into
K
-
π
+
is determined to be
A
K
π
=
0.132
±
0.011
±
0.007
, where the first uncertainty is statistical and the second is systematic. This measurement is an update of an earlier study exploiting additional tagging modes, including several decay modes involving a
K
L
0
meson. The branching fractions of the
K
L
0
modes are determined as input to the analysis in a manner that is independent of any strong phase uncertainty. Using the predominantly
C
P
-even tag
D
→
π
+
π
-
π
0
and the ensemble of
C
P
-odd eigenstate tags, the observable
A
K
π
π
π
π
0
is measured to be
0.130
±
0.012
±
0.008
. The two asymmetries are sensitive to
r
D
K
π
cos
δ
D
K
π
, where
r
D
K
π
and
δ
D
K
π
are the ratio of amplitudes and phase difference, respectively, between the doubly Cabibbo-suppressed and Cabibbo-favoured decays. In addition, events containing
D
→
K
-
π
+
tagged by
D
→
K
S
,
L
0
π
+
π
-
are studied in bins of phase space of the three-body decays. This analysis has sensitivity to both
r
D
K
π
cos
δ
D
K
π
and
r
D
K
π
sin
δ
D
K
π
. A fit to
A
K
π
,
A
K
π
π
π
π
0
and the phase-space distribution of the
D
→
K
S
,
L
0
π
+
π
-
tags yields
δ
D
K
π
=
187.6
-
9.7
+
8.9
-
6.4
+
5.4
∘
, where external constraints are applied for
r
D
K
π
and other relevant parameters. This is the most precise measurement of
δ
D
K
π
in quantum-correlated
D
D
¯
decays.
