Red-osier dogwood, a native species of flowering plant in North America, has been reported to have anti-oxidative properties because of abundant phenolic compounds; this could be promising as a ...functional food or a feed additive. In the present study, an oxidative damage model using 1.0 mM hydrogen peroxide (H2O2) in Caco-2 cells was established to evaluate the antioxidative effects of red-osier dogwood extracts (RDE). The results showed that 1.0 mM H2O2 pre-exposure for 3 h significantly decreased cell viability, and increased interleukin 8 (IL-8) secretion and the intracellular reactive oxygen species (ROS) level. Caco-2 cells were treated with 100 µg/mL RDE for 24 h after pre-exposure to H2O2. It was found that the decreased cell viability caused by H2O2 was significantly restored by a subsequent 100 µg/mL RDE treatment. Furthermore, the IL-8 secretion and ROS level were significantly blocked by RDE, accompanied by the enhanced gene expression of hemeoxygenase-1 (HO-1), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px), and the enhanced protein expression of the nuclear factor (erythroid-derived 2)-like 2 (Nrf-2). Moreover, RDE improved barrier functions in Caco-2 cells. Using RDE reduced the diffusion of fluorescein isothiocyanate (FITC)-dextran and increased the transepithelial resistance (TEER) value. The relative mRNA level of tight junction claudin-1, claudin-3, and occludin was elevated by RDE. These extracts also repaired the integrity of zonula occludens-1 (ZO-1) damaged by H2O2 and increased the protein expressions of ZO-1 and claudin-3 in the H2O2-pretreated cells. These results illustrated that RDE reduced the ROS level and enhanced the barrier function in oxidative-damaged epithelial cells.
Red-osier dogwood extracts (RDE) contain high levels of phenolic compounds which have been recognized as natural antioxidants. In this study, the potential of RDE to prevent cardiovascular diseases ...(CVDs) was evaluated using Caco-2 cells and a co-culture model of Caco-2 BBe1/EA.hy926 cells in Transwell® plates. The results showed that RDE supplementation significantly prevented interleukin-8 (IL-8) production and suppressed the gene expression of IL-8, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and cyclooxygenase 2 (COX-2) in the TNF-α inflamed Caco-2 cells. Meanwhile, the polyphenols (quercetin-3-glucoside, quercetin-glucuronide, rutin, quercetin-3-O-malonylglucoside, and kaempferol-glucoside) in the RDE were validated to be absorbed by Caco-2 BBe1 cells and transported to the basal chamber where EA.hy926 cells were located during 12 h incubation. The transported polyphenols were able to prevent IL-8 production and suppress the gene expression of proinflammatory mediators (TNF-α, ICAM-1, VCAM-1, and COX-2) in the TNF-α or oxidized low-density lipoprotein (ox-LDL) treated EA.hy926 cells. These novel findings demonstrated that phenolic compounds in RDE can be transported to the cardiovascular system by intestinal absorption and mitigate the inflammatory responses of vascular endothelial cells, indicating that RDE could be a natural resource of polyphenols to prevent inflammation cytokine or oxidized lipid-induced CVDs.
Deoxynivalenol (DON) contamination occurs in feeds and causes a reduction in growth performance, damage to the intestinal epithelial cells, and increased susceptibility to enteric pathogen challenge. ...Sodium metabisulfite (SMBS) has shown promise in reducing DON; however, SMBS quickly degrades under aqueous acidic conditions such as the environment within a stomach. Thus, protection of SMBS is required for effective delivery to the small intestine to detoxify DON. This study was to encapsulate SMBS into hydrogenated palm oil-based microparticles for its delivery to the small intestine and to evaluate its efficacy on DON detoxification in simulated intestinal fluids using IPEC-J2 cells in vitro. The diameter of the SMBS containing microparticles was 511 ± 135 μm, and the loading capacity of SMBS in the microparticles was 45.50%; 1.41% of the encapsulated SMBS (ES) was released into the simulated gastric fluid, and 66.39% of ES was progressively released into the simulated intestinal fluid within 4 h at 37 °C. In IPEC-J2 cells, when DON was treated with the simulated gastric fluid containing 0.5% ES for 2 h, then mixed with the simulated intestinal fluid (1:1) and incubated for 2 h, cytotoxicity was not observed. DON treated with 0.5 ES decreased the gene expression of inflammatory cytokines in the cells compared with DON alone and maintained the cell integrity. To conclude, the SMBS containing microparticles were stable in the simulated gastric fluid and allowed a progressive release of SMBS in the simulated intestinal fluid. The released SMBS in the simulated intestinal fluid effectively detoxified DON.
Abstract
Probiotics have long been used in food animal production to improve animal health and nutrition. This practice has received an extensive interest after European Union countries restricted ...the use of antibiotics as animal growth promoters (AGP). Although probiotics have good potential to replace AGPs by offering various benefits to animal hosts in general, their efficacy and consistency remain to be a challenge in application. Developing scientific evidence-based probiotics is thus critical to resolve the issue. The current presentation points out the major gaps in the development and application of probiotics and is focused on the tips that can be used to select novel probiotic isolates with efficacy and specificity. These have been illustrated by the authors’ research. For instance, the efficiency of probiotic selection is largely limited by the use of farm animals and how a lab animal model can be used to speed up the selection and reveal the molecular mechanisms underlying probiotic effects. The potential of advanced genomic approaches in identifying such probiotics and determining their molecular mechanisms is also discussed using an unique example in the field, which is relevant to the control of diarrhea in piglets.
Probiotics have long been used in food animal production to improve animal health and nutrition. This practice has received an extensive interest after European Union countries restricted the use of ...antibiotics as animal growth promoters (AGP). Although probiotics have good potential to replace AGPs by offering various benefits to animal hosts in general, their efficacy and consistency remain to be a challenge in application. Developing scientific evidence-based probiotics is thus critical to resolve the issue. The current presentation points out the major gaps in the development and application of probiotics and is focused on the tips that can be used to select novel probiotic isolates with efficacy and specificity. These have been illustrated by the authors' research. For instance, the efficiency of probiotic selection is largely limited by the use of farm animals and how a lab animal model can be used to speed up the selection and reveal the molecular mechanisms underlying probiotic effects. The potential of advanced genomic approaches in identifying such probiotics and determining their molecular mechanisms is also discussed using an unique example in the field, which is relevant to the control of diarrhea in piglets.
The present study investigated the effects of iron, iron chelators, and mutations of tonB or iroN fepA genes on the growth and virulence of Salmonella Typhimurium. Results indicated that organic iron ...(ferric citrate and ferrous-l-ascorbate) supported better growth of Salmonella compared to inorganic iron. Among tested chelators, 2,2′-bipyridyl at 500 μM showed the highest inhibition of Salmonella growth with 5 μM ferrous sulfate. Deletion of genes (tonB– and iroN– fepA– ) in the iron uptake system attenuated Salmonella invasion of Caco-2 cells and its ability to damage the epithelial monolayer. The expression of all tested host genes in Caco-2 was not affected under the iron-poor condition. However, claudin 3, tight junction protein 1, tumor necrosis factor α (TNF-α), and interleukin-8 (IL-8) were altered under the iron-rich condition depending on individual mutations. In Caenorhabditis elegans, a significant down-regulation of ferritin 1 expression was observed when the nematode was infected by the wild-type (WT) strain.
In order to investigate the anti-inflammatory activity of egg yolk-derived phosphopeptides (PPPs), intestinal epithelial (Caco-2 cells, HT-29 cells) and macrophage RAW 264.7 cell lineages were ...stimulated with either tumor necrosis factor alpha (TNF-α) or Salmonella enterica lipopolysaccharide (LPS). Upon pre-incubation with different doses of PPPs, the secretion of interleukin 8 (IL-8) and TNF-α in cell supernatants was quantified by enzyme-linked immunosorbent assay (ELISA). The gene expression levels of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines (i.e., IL-8, IL-12, IL-6, monocyte chemoattractant protein-1 (MCP-1), TNF-α, IL-1β) were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results revealed that PPPs significantly inhibit the secretion of IL-8 in TNF-α-stimulated Caco-2 and HT-29 cells in a dose-dependent manner; while no significant difference was observed in LPS-stimulated Caco-2 and HT-29 cells. Interestingly, the expression of IL-8, MCP-1 and IL-12 was inhibited by PPPs in both TNF-α- and LPS-stimulated HT-29 cells, and similarly, the expression of TNF-α, IL-1β, IL-6, and iNOS was downregulated significantly in LPS-stimulated macrophage RAW 264.7 cells pre-incubated with PPPs. We conclude that PPPs possesses anti-inflammatory properties evidenced by the down-regulation of key pro-inflammatory markers. Thus our results suggest that PPPs may serve as nutraceutical compounds with anti-inflammatory properties.
Significant variation in the birth weight of piglets has arisen due to increased sow prolificacy. Intestinal development and function may be affected by birth weight. Low birth weight (LBW) pigs may ...also have reduced feed intake, leading to further impairment of intestinal development. The objective of this study was to examine the intestinal development pattern of LBW and normal birth weight (NBW) piglets with normal nutrition (NN) or restricted nutrition (RN) in the pre-weaning period. Jejunal intestinal samples were analyzed for target gene expression and enzyme activity at d 28 (weaning) and d 56 (post-weaning). At d 28, excitatory amino acid transporter (EAAC1) and sodium-dependent neutral amino acid transporter (B0AT1) were downregulated in LBW compared to NBW pigs (p < 0.05). On d 56, B0AT1 and ASCT2 (glutamine transporter) were downregulated in RN compared to NN pigs (p < 0.05), regardless of birth weight. Peptide transporter 1 (PepT1) expression was downregulated in LBW compared to NBW pigs at 28 d (p < 0.05), with no effects of treatments at 56 d. Sodium-glucose transporter-1 (SGLT1) was upregulated in NBW-NN compared to LBW-NN pigs (p < 0.05) at 28 d. Alkaline phosphatase (ALP) was upregulated in LBW-RN at d 28. At d 56, claudin-3 (CLDN-3) and Zonular occludin-1 (ZO-1) were upregulated in NN compared to RN pigs (p < 0.05). There were no treatment effects on ALP, maltase, or sucrase activity at 28 d. However, at 56 d, ALP was upregulated in NBW-NN pigs while sucrase activity was upregulated in NN pigs (p < 0.05). The results demonstrate differences in jejunal gene expression associated with birth weight, with reduced gene expression of amino acid transporters (PepT1, EAAC1, B0AT1) in LBW compared to NBW pigs (p < 0.05). While neonatal nutrient restriction had minimal effects at 28 d and d 56 for tight junction protein transcript abundance, neutral amino acid transporter abundance was upregulated in NN pigs compared to RN piglets (p < 0.05).
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