The development of a method for identification and determination of thiamphenicol by Fourier Transform Infrared will provide convenience to developers because it is fast and easy for analysis. The ...research was carried out by utilizing the solubility of thiamphenicol in methanol with three stages, namely method development, sample analysis, and method validation. The method development stage showed that the specific peak of thiamphenicol was at a peak with a wavenumber of 1694.1 cm−1; this specific peak of thiamphenicol was used for qualitative analysis and quantitative analysis of thiamphenicol in the capsule dosage form. The sample analysis showed that all analyzed thiamphenicol in capsule dosage form showed good results both qualitatively and quantitatively. Qualitatively all the samples analyzed showed a specific peak at specific positions and specific wavenumbers. These results meet the requirements for containing thiamphenicol in the dosage form. Quantitatively all the samples analyzed ranged from 97.97% to 102.24% by peak height and peak area. These results meet the requirements for active substance levels in general preparations within 90.0% to 110.0%. The method validation for peak height and peak area showed that the accuracy parameter had a recovery percentage of 100.28% and 100.41% (between 98.0% to 102.0%), the precision parameter with a relative standard deviation of 0.31% and 0.37% (not more than 2.0%), and the linearity parameter with a correlation coefficient of 0.9999 and 0.9997 (not less than 0.99). The limit of detection value was 0.2971 mg/mL and 0.5338 mg/mL, the limit of quantitation value was 0.9004 mg/mL and 1.6176 mg/mL, the range for both was 80% to 120%, and the specificity for both met the requirement. The Fourier Transform Infrared method has been successfully developed, applied, and validated for qualitative analysis and quantitative analysis of thiamphenicol in capsule dosage form.
Ketapang is one of the many medicinal plant species that grow in Indonesia and is used to treat various diseases. Ketapang leaves contain flavonoids, tannins, saponins, and terpenoids that have ...anti-inflammatory, antioxidant, antiviral, and antimicrobial properties. This study aimed to determine the LD50 and histopathology of Liver and kidney damage before the formulation of tablets containing galactomannan-binding agents. The toxicity determination method was carried out in vivo in experimental animals at doses of 4g/kg BW, 8g/kg BW and 16g/kg BW, and Liver and kidney histopathology was carried out before formulation into tablet preparations using the wet granulation method with various concentrations of binders and disintegrants, namely F1(8:0), F2(0:8), F3(4:4), F4(2:6), and F5(6:2). The results of the toxicity test showed an LD50 of 15.9959, liver damage at a dose of 4 g/kg BW hepatocyte karyorrhexis cells, central vein constriction, sinusoidal dilatation, a dose of 8 g/kg BW hepatocyte karyorrhexis, significant venous congestion, sinusoidal dilatation, a dose of 16 g/kg BW hepatocyte cells, karyolysis, dilated central veins, and dilated sinusoids. The results of the tablet mass preformulation test meet the requirements: the flow time test was 1.48-2.14 g/second, the angle of repose test was 24.60°-30.60°, and the tab index test was 5.33%-9.33%. The results of the tablet evaluation test were as follows: the tablet hardness test was 3.8-8.6 kg, the tablet friability test was 0.167-0.64%, and the tablet disintegration time test was 29.06-107.51 minutes.
Diarrhea is one type of disease with the most sufferers every year. Thus, it is considered an endemic disease in Indonesia and the potential disease of Extraordinary Events accompanied by mortality. ...One of the plants that can be used as a traditional medicine for diarrhea is the avocado seed, as it contains tannins, alkaloids, flavonoids, steroids and glycosides, which act as antidiarrheals. This study aims to determine the antidiarrheal effect of avocado seed extract suspension on mice induced by Oleum ricini and the optimum concentration of avocado seed extract suspension and capsules with an antidiarrheal effect in white male mice induced by Oleum ricini. The dosage forms chosen were suspension and capsules. This study used an experimental method with a test sample of the avocado seed. Avocado seed Simplicia was extracted by the percolation method, then an oral suspension and capsule formulation were made from the avocado seed methanol extract. It was evaluated and tested for its effectiveness with mice to cure diarrhea. Normal data were analyzed by One Way ANOVA and Post Hoc Tuckey Method. The results of this study showed that the administration of a suspension of avocado seed methanol extract at a dose of 800 mg/KgBB had the most optimum effect as an antidiarrheal against white male mice with a stool weight of 0.39 grams and a duration of diarrhea for 74 minutes. Furthermore, the administration of avocado seed extract capsules at a dose of 75 mg/KgBB had the most optimum effect as antidiarrheal against white male mice with Loperamide as a positive control. Therefore, it can be concluded that all suspension formulations and capsules of avocado seed methanol extract met the requirements for preparation evaluation. Suspension and avocado seed methanol extract capsules can cure diarrhea in white male mice.
Objective: This research aims to apply the Visual Basic.Net (VB.NET) of individual dose calculations based on the formula of pharmacokinetics for diabetic patients of chronic renal disorder ...complication in Dr. Pirngadi Hospital because the dosage administered was not based on the patient’s creatinine clearance.Methods: This descriptive research was conducted using a simulated creatinine cleavage calculation using VB.NET programming language applications with variable patient data, the value of creatinine, the name of drug, and dosage.Results: This study obtained data about the use of drugs 40 patients who met the inclusion criteria of 320 medical records of diabetic patients, there are 6 types of drugs that are not in accordance with the dose based on the calculation of creatinine clearance are ceftriaxone(18 of 18 cases), furosemide (19 of 19 cases), ciprofloxacin (2 of 8 cases), ranitidine (4 of 24 cases), metformin (2 of 7 cases), and captopril (16 of 16 cases).Conclusions: This research aims to apply the VB.NET is it able to apply individual doses for patients with diabetes complications of renal failure have not been applied in accordance with creatinine clearance calculations at this hospitalwhere this work is difficult to do.
Background: Coconut shell is often used for cooking or treated as waste. Liquid smoke is a by-product of coconut shell processing, which results from condensation of combustion steam. It is divided ...into grades one, two, and three based on the characteristics and utilisation. It contains phenolic, carbonyl acids and other compounds which act as antioxidants, antibacterials, and antifungals.
Objective: To determine the total content of flavonoids, antioxidants and antibacterial activity in liquid smoke.
Method: Liquid smoke was produced by a pyrolysator and distilled to produce grades one, two and three. It was then tested for antibacterial activity using the minimal inhibitory concentration (MIC) method against S. aureus and E. coli. The AlCl3 reagent and DPPH method were used to determine the total flavonoid and antioxidant levels, respectively.
Results: The MIC of grade one, two, and three coconut shell liquid smoke was 10%; 10%; 5% against S. aureus; and 10%, 5%, and 5% against E. coli. The total flavonoid yields from grades one, two and three were 0.0041, 0.0174, and 0.2741 mg QE/ml, respectively. Test results for antioxidant grades with IC50 values were 142.82, 121.64, and 90.80 µg/mL.
Conclusion: Coconut shell liquid smoke has antibacterial activity, contains a certain amount of flavonoids, and also possesses antioxidant activity.
Abstract Solanum erianthum is one of the herbal plants that is believed by the Indonesian people to be a medicinal plant. The flavonoid and polyphenol compounds contained therein have antioxidant ...activity, but this has not been proven empirically. Solanum erianthum extract is produced using the maceration method. The determination of total flavonoid levels in Solanum erianthum was measured using a method based on complex formation, namely the aluminum trichloride (AlCl3) method. The antioxidant activity of Solanum erianthum was measured using the DPPH (1,1-diphenyl-2-2picrihydrazyl) method because of its stability in the form of free radicals and because it is simple to carry out. The results obtained after determining the total flavonoid content in Solanum erianthum extract with various concentrations using the aluminum trioxide method were 1,430 mg QE/g, 2,282 mg QE/g, 3,180 mg QE/g, 3,567 mg QE/g, 4,291 mg QE/g. Solanum erianthum extract demonstrated antioxidant activity in the DPPH test, with an IC50 value of 66.493 g/ml. This falls into the “strong” category. This research shows that the phytochemical screening content of Solanum erianthum extract contains alkaloids, flavonoids, tannins, saponins, and tritepenoids. The total flavonoid content in Solanum erianthum extract at concentrations of 6 µg/ml, 8 µg/ml, 10 µg/ml, 12 µg/ml, and 14 µg/ml is as follows: 1,430mg QE/g, 2,282mg QE/g, 3,180mg QE/g, 3,567mg QE/g, and 4,291mg QE/g, as well as the antioxidant activity of Solanum erianthum extract with an average yield of 69,493 ppm.
Traditional medicine is currently being explored and developed widely by various countries because its cost effectiveness and reduced side effects. The green betel (Piper betle Linn.) and the red ...betel (Piper crocatum Ruiz and Pav.) are plants from the same Piper genus that are widely cultivated in Indonesia and have various phytochemical properties and pharmacological effects. The objective of this research is to determine the cytotoxicity of green leaves and red betel leaves. This research was conducted in two main stages: first, the extraction and preparation through the soxhletation method; second, a cytotoxicity test through the brine shrimp (Artemia salina Leach) lethality test (BSLT). The results showed that there were lethal concentration 50 % (LC50) value in both green betel leaves ethanolic extract and red betel leaves ethanolic extract, with 44.975 μg per mL and 31.556 μg per mL, respectively. The red betel (leaves ethanolic extract has a higher cytotoxicity than green betel (leaves ethanolic extract which can be seen from the lower lethal concentration of 50% (LC50) value. The difference in cytotoxicity is due to differences in plant varieties that cause variations in phytochemical content which affects the pharmacological effects. The green betel leaves ethanolic extract and the red betel leaves ethanolic extract are cytotoxic and have potential anticancer properties.
PDF
