Multi-criteria decision analysis (MCDA) methods have become increasingly popular in decision-making for sustainable energy because of the multi-dimensionality of the sustainability goal and the ...complexity of socio-economic and biophysical systems. This article reviewed the corresponding methods in different stages of multi-criteria decision-making for sustainable energy, i.e., criteria selection, criteria weighting, evaluation, and final aggregation. The criteria of energy supply systems are summarized from technical, economic, environmental and social aspects. The weighting methods of criteria are classified into three categories: subjective weighting, objective weighting and combination weighting methods. Several methods based on weighted sum, priority setting, outranking, fuzzy set methodology and their combinations are employed for energy decision-making. It is observed that the investment cost locates the first place in all evaluation criteria and CO
2 emission follows closely because of more focuses on environment protection, equal criteria weights are still the most popular weighting method, analytical hierarchy process is the most popular comprehensive MCDA method, and the aggregation methods are helpful to get the rational result in sustainable energy decision-making.
The technical, economical and environmental performances of combined cooling, heating and power (CCHP) system are closely dependent on its design and operation strategy. This paper analyzes the ...energy flow of CCHP system and deduces the primary energy consumption following the thermal demand of building. Three criteria, primary energy saving (PES), annual total cost saving (ATCS), and carbon dioxide emission reduction (CDER) are selected to evaluate the performance of CCHP system. Based on the energy flow of CCHP system, the capacity and operation of CCHP system are optimized by genetic algorithm (GA) so as to maximize the technical, economical and environmental benefits achieved by CCHP system in comparison to separation production system. A numerical example of gas CCHP system for a hotel building in Beijing is given to ascertain the effectiveness of the optimal method. Furthermore, a sensitivity analysis is presented in order to show how the optimal operation strategy would vary due to the changes of electricity price and gas price.
The design and operation of combined cooling, heating and power (CCHP) systems are greatly dependent upon the seasonal atmospheric conditions, which determine thermal and power demands of buildings. ...This paper presents a mathematical analysis of CCHP system in comparison to separate system. The corresponding primary energy consumption in thermal demand management (TDM) and electrical demand management (EDM) operation modes are deduced. Three relative criteria, primary energy saving (PES), CO
2 emission reduction (CO
2ER), and annual total cost saving (ATCS) are employed to evaluate the respective performances of CCHP systems for a hypothetical building in five different climate zones from the technical, environmental and economic aspects. The results indicate that CCHP system in TDM mode in the cold area, where the building requires more heating during the year, achieves more benefit over separate system while CCHP system in EDM mode suits the building having stable thermal demand in mild climate zone.
Notch receptors (Notch1-4) play critical roles in tumorigenesis and metastasis of malignant tumors, including breast cancer. Although abnormal Notch activation is related to various tumors, the ...importance of single receptors and their mechanism of activation in distinct breast cancer subtypes are still unclear. Previous studies by our group demonstrated that Notch3 may inhibit the emergence and progression of breast cancer. PTEN is a potent tumor suppressor, and its loss of function is sufficient to promote the occurrence and progression of tumors. Intriguingly, numerous studies have revealed that Notch1 is involved in the regulation of PTEN through its binding to CBF-1, a Notch transcription factor, and the PTEN promoter. In this study, we found that Notch3 and PTEN levels correlated with the luminal phenotype in breast cancer cell lines. Furthermore, we demonstrated that Notch3 transactivated PTEN by binding CSL-binding elements in the PTEN promoter and, at least in part, inhibiting the PTEN downstream AKT-mTOR pathway. Notably, Notch3 knockdown downregulated PTEN and promoted cell proliferation and tumorigenesis. In contrast, overexpression of the Notch3 intracellular domain upregulated PTEN and inhibited cell proliferation and tumorigenesis in vitro and in vivo. Moreover, inhibition or overexpression of PTEN partially reversed the promotion or inhibition of cell proliferation induced by Notch3 alterations. In general, Notch3 expression positively correlated with elevated expression of PTEN, ER, lower Ki-67 index, and incidence of involved node status and predicted better recurrence-free survival in breast cancer patients. Therefore, our findings demonstrate that Notch3 inhibits breast cancer proliferation and suppresses tumorigenesis by transactivating PTEN expression.
Abstract
Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel β-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated ...with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection.
Methods
In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2.
Results
To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively.
Conclusions
Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.
A peptide-based magnetic chemiluminescence enzyme immunoassay for the detection of SARS-CoV-2 antibodies was developed; 71.4% (197 of 276) and 57.2% (158 of 276) of the COVID-19 inpatients were positive for IgG and IgM against SARS-CoV-2.
► The performances of CCHP system in different operation modes are compared. ► The CCHP system following electrical load and thermal loads are analyzed. ► The instantaneous performances of CCHP ...system with loads are obtained. ► The sensitivity of CCHP system to the technical parameters are analyzed.
Operation mode of combined cooling heating and power (CCHP) system determines its energetic and environmental performances. This paper analyzes the energy flows of CCHP system and separated production (SP) system. The fuel energy consumptions of CCHP system following electrical demand management (EDM) and thermal demand management (TDM) are deduced respectively. Three indicators: primary energy saving, exergy efficiency and CO
2 emission reduction, are employed to evaluate the performances of CCHP system for a commercial building in Beijing, China. The feasibility analysis shows that the performance of CCHP system is strictly dependent upon building energy demands. The selection of CCHP operation modes is systemically based on building loads, CCHP system and local SP system. The calculation results conclude that CCHP system in winter under EDM achieves more benefits than in summer. The sensitivity discussion indicates that the coefficient of performance for cooling and the efficiency of electricity generation are the most sensitive variables to the energetic and environmental performances of CCHP system.
The clinical benefits of steroid therapy during cardiac arrest (CA) are unclear. Several recent clinical trials have shown that administering corticosteroid therapy during CA may improve patient ...outcomes. The purpose of the present study was to determine whether providing corticosteroids improves outcomes for patients following CA.
We searched the PubMed, Embase, Cochrane Library, Web of Science and CNKI databases for randomized controlled trials comparing corticosteroid therapy to placebo during CA.
Eleven relevant studies involving a total of 2273 patients were included in the meta-analysis. The statistical analysis showed that corticosteroid treatment during CA was significantly associated with an increased rate of sustained return of spontaneous circulation (ROSC) (OR: 2.05, 95% CI: 1.24 to 3.37, P < 0.01). Corticosteroid treatment during CA did not show a significant benefit in favorable neurological outcomes (OR: 1.13, 95% CI: 0.81 to 1.58, P = 0.49) or overall survival rate at hospital discharge (OR: 1.29, 95% CI: 0.74 to 2.26, P = 0.38). However, in the subgroup analysis, we found that patients had a significantly increased survival rate and ROSC if the dose of corticosteroid therapy above 100 mg methylprednisolone. The statistical analysis revealed no significant differences in adverse events.
High-dose corticosteroid treatment (above 100 mg methylprednisolone) is associated with better overall survival rate at hospital discharge and ROSC outcomes. However, there is uncertainty regarding whether this treatment results in a benefit or harm to the favorable neurological outcomes at hospital discharge.
Extensive clinical trials indicate that patients with negative sentinel lymph node biopsy do not need axillary lymph node dissection (ALND). However, the ACOSOG Z0011 trial indicates that patients ...with clinically negative axillary lymph nodes (ALNs) and 1-2 positive sentinel lymph nodes having breast conserving surgery with whole breast radiotherapy do not benefit from ALND. The aim of this study is therefore to identify those patients with 0-2 positive nodes who might avoid ALND. A total of 486 patients were eligible for the study with 212 patients in the modeling group and 274 patients in the validation group, respectively. Clinical lymph node status, histologic grade, estrogen receptor status, and human epidermal growth factor receptor 2 status were found to be significantly associated with ALN metastasis. A negative binomial regression (NBR) model was developed to predict the probability of having 0-2 ALN metastases with the area under the curve of 0.881 (95% confidence interval 0.829-0.921, P < 0.001) in the modeling group and 0.758 (95% confidence interval 0.702-0.807, P < 0.001) in the validation group. Decision curve analysis demonstrated that the model was clinically useful. The NBR model demonstrated adequate discriminative ability and clinical utility for predicting 0-2 ALN metastases.
Astaxanthin (AST), a natural antioxidant carotenoid, has been shown to exert anti-inflammatory effects. However, to our knowledge, no study has specifically addressed the potential protective effects ...of AST against bovine endometritis. The purpose of this study was to examine whether treatment with AST could protect endometrial epithelial cells against lipopolysaccharide (LPS)-induced inflammatory injury. Treatment of bovine endometrial (BEND) epithelial cell line with AST reduced LPS-induced production of interleukin-6 and tumor necrosis factor-alpha, increased the cellular activity of superoxide dismutase and catalase, decreased the proportion of apoptotic cells, and promoted the production of insulin-like growth factor and epithelial growth factor. The effects of AST were mediated through the downregulation of B-cell lymphoma 2 (Bcl-2) associated X, apoptosis regulator (Bax), and cleaved caspase-3 and through the upregulation of Bcl-2. Moreover, AST significantly increased the expression of the tight junction proteins (TJP) claudin, cadherin-1, and TJP1, which play an essential role in the maintenance of host endometrial defense barrier against pathogen infection. Collectively, these results demonstrated that treatment with AST protected against oxidative stress, prevented cell apoptosis, promoted BEND cells viability, and increased the production of growth factors, in addition to activating the endometrial defense barrier. Therefore, AST is a promising therapeutic agent for the prevention and treatment of endometritis. This finding is of utmost importance in the present times when the excessive use of antibiotics has resulted in the development of antibiotic-resistant bacteria. Summary sentence Astaxanthin counteracts LPS-induced inflammation, oxidative stress, apoptosis, and tight junction disassembly in bovine endometrial epithelial cells, and is a potential therapeutic agent against bovine endometritis.
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