Ischemic stroke can induce changes in mitochondrial morphology and function. As a regulatory gene in mitochondria, optic atrophy 1 (OPA1) plays a pivotal role in the regulation of mitochondrial ...dynamics and other related functions. However, its roles in cerebral ischemia-related conditions are barely understood.
Cultured rat primary cortical neurons were respectively transfected with OPA1-v1ΔS1-encoding and OPA1-v1-encoding lentivirus before exposure to 2-h oxygen-glucose deprivation (OGD) and subsequent reoxygenation (OGD/R). Adult male SD rats received an intracranial injection of AAV-OPA1-v1ΔS1 and were subjected to 90 min of transient middle cerebral artery occlusion (tMCAO) followed by reperfusion. OPA1 expression and function were detected by in vitro and in vivo assays.
OPA1 was excessively cleaved after cerebral ischemia/reperfusion injury, both in vitro and in vivo. Under OGD/R condition, compared with that of the LV-OPA1-v1-treated group, the expression of OPA1-v1ΔS1 efficiently restored L-OPA1 level and alleviated neuronal death and mitochondrial morphological damage. Meanwhile, the expression of OPA1-v1ΔS1 markedly improved cerebral ischemia/reperfusion-induced motor function damage, attenuated brain infarct volume, neuronal apoptosis, mitochondrial bioenergetics deficits, oxidative stress, and restored the morphology of mitochondrial cristae and mitochondrial length. It also preserved the mitochondrial integrity and reinforced the mtDNA content and expression of mitochondrial biogenesis factors in ischemic rats.
Our results demonstrate that the stabilization of L-OPA1 protects ischemic brains by reducing neuronal apoptosis and preserving mitochondrial function, suggesting its significance as a promising therapeutic target for stroke prevention and treatment.
The safe storage and sharing of medical data have promoted the development of the public medical field. At the same time, blockchain technology guarantees the safe storage and sharing of medical ...data. However, the consensus algorithm in the current medical blockchain cannot meet the requirements of low delay and high throughput in the large-scale network, and the identity of the primary node is exposed and vulnerable to attack. Therefore, this paper proposes an efficient consensus algorithm for medical data storage and sharing based on a master–slave multi-chain of alliance chain (ECA_MDSS). Firstly, institutional nodes in the healthcare alliance chain are clustered according to geographical location and medical system structure to form a multi-zones network. The system adopts master–slave multi-chain architecture to ensure security, and each zone processes transactions in parallel to improve consensus efficiency. Secondly, the aggregation signature is used to improve the practical Byzantine fault-tolerant (PBFT) consensus to reduce the communication interaction of consensus in each zone. Finally, an efficient ring signature is used to ensure the anonymity and privacy of the primary node in each zone and to prevent adaptive attacks. Meanwhile, a trust model is introduced to evaluate the trust degree of the node to reduce the evil done by malicious nodes. The experimental results show that ECA_ MDSS can effectively reduce communication overhead and consensus delay, improve transaction throughput, and enhance system scalability.
Abstract
When and where the magnetic field energy is released and converted in eruptive solar flares remains an outstanding topic in solar physics. To shed light on this question, here we report ...multiwavelength observations of a C9.4-class eruptive limb flare that occurred on 2017 August 20. The flare, accompanied by a magnetic flux rope eruption and a white light coronal mass ejection, features three post-impulsive X-ray and microwave bursts immediately following its main impulsive phase. For each burst, both microwave and X-ray imaging suggest that the nonthermal electrons are located in the above-the-loop-top region. Interestingly, contrary to many other flares, the peak flux of the three post-impulsive microwave and X-ray bursts shows an increase for later bursts. Spectral analysis reveals that the sources have a hardening spectral index, suggesting a more efficient electron acceleration into the later post-impulsive bursts. We observe a positive correlation between the acceleration of the magnetic flux rope and the nonthermal energy release during the post-impulsive bursts in the same event. Intriguingly, different from some other eruptive events, this correlation does not hold for the main impulse phase of this event, which we interpret as energy release due to the tether-cutting reconnection before the primary flux rope acceleration occurs. In addition, using footpoint brightenings at conjugate flare ribbons, a weakening reconnection guide field is inferred, which may also contribute to the hardening of the nonthermal electrons during the post-impulsive phase.
Abstract
We present a multiwavelength analysis of two flare-related jets on 2014 November 13, using data from the Solar Dynamics Observatory/Atmospheric Imaging Assembly (SDO/AIA), the Reuven High ...Energy Solar Spectroscopic Imager (RHESSI), the Hinode/X-ray Telescope (XRT), and the Interface Region Imaging Spectrograph (IRIS). Unlike most coronal jets, where hard X-ray (HXR) emissions are usually observed near the jet base, in these events HXR emissions are found at several locations, including in the corona. We carry out the first differential emission measure analysis that combines both AIA (and XRT, when available) bandpass filter data and RHESSI HXR measurements for coronal jets, and obtain self-consistent results across a wide temperature range and into nonthermal energies. In both events, hot plasma first appears at the jet base, but as the base plasma gradually cools, hot plasma also appears near the jet top. Moreover, nonthermal electrons, while only mildly energetic, are found in multiple HXR locations and contain large amounts of total energy. In particular, the energetic electrons that produce the HXR sources at the jet top are accelerated near the top location, rather than traveling from a reconnection site at the jet base. This means that there is more than one particle acceleration site in each event. Jet velocities are consistent with previous studies, including the upward and downward velocities around ∼200 km s
−1
and ∼100 km s
−1
, respectively, and fast outflows of 400–700 km s
−1
. We also examine the energy partition in the later event, and find that the nonthermal energy in the accelerated electrons is most significant compared to the other energy forms considered. We discuss the interpretations and provide constraints on the mechanisms for coronal jet formation.
The colonic diaphragm in an infant Zhao, Xiaoli; Zhang, Yixian; Zhang, Libing ...
Asian journal of surgery,
October 2022, 2022-10-00, 2022-10-01, Letnik:
45, Številka:
10
Journal Article
Abstract Where and how flares efficiently accelerate charged particles remains an unresolved question. Recent studies revealed that a “magnetic bottle” structure, which forms near the bottom of a ...large-scale reconnection current sheet above the flare arcade, is an excellent candidate for confining and accelerating charged particles. However, further understanding its role requires linking the various observational signatures to the underlying coupled plasma and particle processes. Here we present the first study combining multiwavelength observations with data-informed macroscopic magnetohydrodynamics and particle modeling in a realistic eruptive flare geometry. The presence of an above-the-loop-top magnetic bottle structure is strongly supported by the observations, which feature not only a local minimum of magnetic field strength but also abruptly slowing plasma downflows. It also coincides with a compact above-the-loop-top hard X-ray source and an extended microwave source that bestrides the flare arcade. Spatially resolved spectral analysis suggests that nonthermal electrons are highly concentrated in this region. Our model returns synthetic emission signatures that are well matched to the observations. The results suggest that the energetic electrons are strongly trapped in the magnetic bottle region due to turbulence, with only a small fraction managing to escape. The electrons are primarily accelerated by plasma compression and facilitated by a fast-mode termination shock via the Fermi mechanism. Our results provide concrete support for the magnetic bottle as the primary electron acceleration site in eruptive solar flares. They also offer new insights into understanding the previously reported small population of flare-accelerated electrons entering interplanetary space.
Abstract
Solar active regions (ARs) contain a broad range of temperatures, with the thermal plasma distribution often observed to peak in the few millions of kelvin. Differential emission measure ...(DEM) analysis can allow instruments with diverse temperature responses to be used in concert to estimate this distribution. Nuclear Spectroscopic Telescope ARray (NuSTAR) hard X-ray (HXR) observations are uniquely sensitive to the highest-temperature components of the corona, and thus extremely powerful for examining signatures of reconnection-driven heating. Here, we use NuSTAR diagnostics in combination with extreme-ultraviolet and soft X-ray observations (from the Solar Dynamics Observatory/Atmospheric Imaging Assembly and Hinode/X-Ray Telescope) to construct DEMs over 170 distinct time intervals during a 5 hr observation of an alternately flaring and quiet active region (NOAA designation AR 12712). This represents the first HXR study to examine the time evolution of the distribution of thermal plasma in an AR. During microflares, we find that the initial microflare-associated plasma heating is predominantly heating of material that is already relatively hot, followed later on by broader heating of initially cooler material. During quiescent times, we show that the amount of extremely hot (>10 MK) material in this region is significantly (∼2–4 orders of magnitude) less than that found in the quiescent AR observed in HXRs by FOXSI-2. This result implies there can be radically different high-temperature thermal distributions in different ARs, and strongly motivates future HXR DEM studies covering a large number of these regions.
PDF
