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•Polystyrene-nanoplastics (PS-NPs) enhance antioxidant activity and activate carbon metabolism.•The difference in root-related gene expressions is induced by PS-NPs.•Jasmonate and ...lignin biosynthesis are inhibited by PS-NPs treatments.•Exogenous JA application alleviates potential adverse effects of PS-NPs on rice seedlings.
Potential adverse effects of nanoplastics (NPs) on marine organisms have received increased attention in recent years. In contrast, few data are available on terrestrial plants, especially on the mechanisms for transport of NPs in plants and phytotoxicity (at both phenotypic and molecular levels) of plants induced by NPs. To address this knowledge gap, we conducted a microcosm study in which hydroponically-cultured rice (Oryza sativa L.) seedlings were exposed to polystyrene (PS)-NPs at 0, 10, 50, and 100 mg L−1 for 16 d and examined for morphological and physiological phenotypes and transcriptomics. Laser confocal scanning micrographs confirmed PS-NPs were uptaken by rice roots, greatly benefitted from the transport activity of aquaporin in rice roots. The significant enhancement (p < 0.05) of antioxidant enzyme activities reflected the oxidative stress response of rice roots upon exposure to PS-NPs. Treatment by PS-NPs decreased root length and increased lateral root numbers. Carbon metabolism was activated (e.g., increased carbon and soluble sugar contents) whereas jasmonic acid and lignin biosynthesis were inhibited. The present study demonstrated the likelihood for transport of PS-NPs in rice roots and induced phytotoxicity by PS-NPs, which should inspire further investigations into the potential human health risks from rice consumption.
LncRNAs regulate metabolism in cancer Lin, Wenyu; Zhou, Qiyin; Wang, Chao-Qun ...
International journal of biological sciences,
01/2020, Letnik:
16, Številka:
7
Journal Article
Recenzirano
Odprti dostop
Metabolic reprogramming is a hallmark of cancer. Mammalian genome is characterized by pervasive transcription, generating abundant non-coding RNAs (ncRNAs). Long non-coding RNAs (lncRNAs) are freshly ...discovered functional ncRNAs exerting extensive regulatory impact through diverse mechanisms. Emerging studies have revealed widespread roles of lncRNAs in the regulation of various cellular activities, including metabolic pathways. In this review, we summarize the latest advances regarding the regulatory roles of lncRNAs in cancer metabolism, particularly their roles in mitochondrial function, glucose, glutamine, and lipid metabolism. Moreover, we discuss the clinical application and challenges of targeting lncRNAs in cancer metabolism. Understanding the complex and special behavior of lncRNAs will allow a better depiction of cancer metabolic networks and permit the development of lncRNA-based clinical therapies by targeting cancer metabolism.
Osteosarcoma is a malignancy that normally affects children, adolescents, and young adults. Although accumulating evidence has demonstrated the importance of HULC in osteosarcoma, little is reported ...about its functional roles and molecular mechanisms.
The expression of HULC and miR-372-3p in osteosarcoma tissues was quantified by qRT-PCR. The regulatory roles of HULC and miR-372-3p on cell proliferation, apoptosis, migration and invasion were determined by CCK-8, colony formation, flow cytometry, wound healing, and transwell assays, respectively. The bioinformatics prediction software RAID v2.0 was used to predict the putative binding sites. The interactions among HULC, miR-372-3p and HMGB1 were explored by luciferase assay and western blot assay.
Our results revealed elevated HULC and decreased miR-372-3p expression in both osteosarcoma tissues and cell lines. Overexpression of HULC or knockdown of miR-372-3p promoted osteosarcoma cell proliferation, migration and invasion and induced cell apoptosis. Bioinformatics and luciferase assays verified that HULC directly interacted with miR-372-3p to attenuate miR-372-3p binding to the HMGB1 3'-UTR. Furthermore, mechanistic investigations confirmed that activation of the miR-372-3p/HMGB1 regulatory loop by knockdown of miR-372-3p or overexpression of HMGB1 reversed the in vitro roles of HULC in promoting osteosarcoma cell proliferation, migration and invasion.
Our study is the first to demonstrate that HULC may act as a ceRNA to modulate HMGB1 expression by competitively sponging miR-372-3p, leading to the regulation of osteosarcoma progression, which provides new insight into osteosarcoma diagnosis and treatment.
Passive daytime radiative cooling (PDRC) with zero energy consumption and pollution is regarded as one of the most feasible solutions to replace conventional electrical cooling in the ...energy-intensive world. Scalable multifunctional radiative cooling materials have great significance to expand the practical applications and advance the progress of this cooling technology, which would bridge the gap between fundamental research and industry. In this review, we focus on the general design strategies with scalable fabrication techniques and multifunction of PDRC materials. Firstly, fundamental principles of PDRC are briefly introduced to evaluate the cooling performance with respect to achieving both high solar reflectance and strong thermal emittance of PDRC coolers. Secondly, the scale-up design strategies of PDRC coolers with various structures and materials are highlighted. Thirdly, the advantages of scalable multifunctional PDRC coolers concerning anti-contamination, durability, energy-saving, intelligence and other characteristics are presented. Finally, diverse commercial applications of scalable PDRC materials in energy-saving building, solar cells cooling, personal thermal management, and water harvesting are discussed. The future-oriented challenges and opportunities of PDRC materials are also indicated. Therefore, this review is envisioned to not only provide fabrication methods of scalable and multi-function PDRC materials, but also put this energy-free cooling technology forward real-world applications.
Glyceraldehyde-3-phosphate dehydrogenase （GAPDH）, initially identified as a glycolytic enzyme and considered as a housekeeping gene, is widely used as an internal control in experiments on proteins, ...mRNA, and DNA. However, emerging evidence indicates that GAPDH is implicated in diverse functions independent of its role in energy metabolism; the expression status of GAPDH is also deregulated in various cancer cells. One of the most common effects of GAPDH is its inconsistent role in the determination of cancer cell fate. Furthermore, studies have described GAPDH as a regulator of cell death; other studies have suggested that GAPDH participates in tumor progression and serves as a new therapeutic target. However, related regulatory mechanisms of its numerous cellular functions and deregulated expression levels remain unclear. GAPDH is tightly regulated at transcriptional and pnsttranscriptional levels, which are involved in the regulation of diverse GAPDH functions. Several cancer-related factors, such as insulin, hypoxia inducible factor-1 （HIF-1）, p53, nitric oxide （NO）, and acetylated histone, not only modulate GAPDH gene expression but also affect protein functions via common pathways. Moreover, posttranslational modifications （PTMs） occurring in GAPDH in cancer cells result in new activities unrelated to the original glycnlytic function of GAPDH. In this review, recent findings related to GAPDH transcriptional regulation and PTMs are summarized. Mechanisms and pathways involved in GAPDH regulation and its different roles in cancer cells are also described.
BackgroundAccumulating studies suggest that targeting epigenetic modifications could improve the efficacy of tumor immunotherapy; however, the mechanisms underlying this phenomenon remain largely ...unknown. Here, we investigated the ability of the epigenetic modifier, enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), to regulate the expression of immune checkpoint inhibitor, programmed death-1 ligand 1 (PD-L1) in hepatocellular carcinoma (HCC).MethodsImmunohistochemistry and multiplex immunofluorescence staining were performed to analyze the expression and correlation of EZH2 and PD-L1 in HCC tissues. Immunoblotting, quantitative real-time PCR, flow cytometry, chromatin immunoprecipitation, and dual-luciferase reporter gene assays were performed to evaluate the regulatory roles of EZH2 on PD-L1 expression.ResultsIn vitro cell experiments revealed that EZH2 negatively regulated the PD-L1 expression of hepatoma cell lines in IFNγ-dependent manner. Mechanistic studies demonstrated that EZH2 could suppress PD-L1 expression by upregulating the H3K27me3 levels on the promoters of CD274 (encoding PD-L1) and interferon regulatory factor 1 (IRF1), an essential transcription factor for PD-L1 expression, without affecting the activation of the IFNγ-signal transducer and activator of transcription 1 (STAT1) pathway. Clinical samples from HCC patients with immune-activated microenvironments showed negative correlations between EZH2 and PD-L1 expression in hepatoma cells. Multivariate Cox analysis demonstrated that the combination of EZH2 and PD-L1 was an independent prognostic factor for both OS and RFS for patients with HCC.ConclusionsThe epigenetic modificator EZH2 can suppress the expression of immune checkpoint inhibitor PD-L1 by directly upregulating the promoter H3K27me3 levels of CD274 and IRF1 in hepatoma cells, and might serve as a potential therapeutic target for combination of immunotherapy for immune-activated HCC.
It is a big challenge to prepare non-rare metal and high-activity electrocatalysts for oxygen reduction reaction (ORR). In this paper, a cobalt/carbon nanotubes/chitosan composite gel was synthesized ...and then annealed under nitrogen atmosphere to yield the cobalt and nitrogen co-modified carbon nanotubes (Co–N-CNTs) nanocomposite electrocatalysts. In this strategy, the cobalt component considerably enhanced the ORR activity and improved the degree of graphitic structure to increase the electronic conductivity. The chitosan served as sustainable source for nitrogen doping. The Co–N-CNTs exhibit excellent oxygen reduction reaction (ORR) electrocatalytic activity due to the synergetic effect of Co species and N-doping. The Co–N-CNTs also deliver excellent methanol tolerance and superior long-term durability to that of commercial Pt/C, making it a promising ORR electrocatalyst.
Recent clinical studies have suggested that programmed death ligand 1 (PD-L1) expression in a tumour could be a potential biomarker for PD-L1/PD-1 blockade therapies.
To better characterise PD-L1 ...expression in hepatocellular carcinoma (HCC), we analysed its expression patterns in 453 HCC patients by double staining for CD68 and PD-L1 using the Tyramide Signal Amplification Systems combined with immunohistochemistry. We also investigated its correlation with clinical features, prognosis and immune status.
The results showed that PD-L1 expression on tumour cells (TCs) was negatively associated with patients' overall survival (OS; P = 0.001) and relapse-free survival (RFS; P = 0.006); however, PD-L1 expression on macrophages (Mφs) was positively correlated with OS (P = 0.017). Multivariate analysis revealed that PD-L1 expression on TCs and Mφs were both independent prognostic factors for OS (hazard ratio (HR) = 1.168, P = 0.004 for TC-PD-L1; HR = 0.708, P = 0.003 for Mφ-PD-L1). Further studies showed that Mφ-PD-L1
tumours exhibited an activated immune microenvironment, with high levels of CD8
T-cell infiltration and immune-related gene expression.
Our study provided a novel methodology to evaluate PD-L1 expression in the tumour microenvironment, which might help to select patients who would benefit from anti-PD-1/PD-L1 immunotherapies.
Coal is the primary source of China’s carbon emissions due to the energy structure and its resource endowment. This reality creates enormous pressure and impetus for low-carbon pathways of coal ...production and consumption. Based on a literature review on carbon emissions accounting methods, this paper builds a source-driven CO
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emissions accounting model for the coal development sector using the emissions factor method. Scenario analysis is employed to predict future carbon emission equivalents and to indicate possible implications for climate change mitigation in this sector. Carbon emissions from coal development are mainly derived from coal mine gas emissions, which yield 62% of the sector’s total carbon emissions, followed by energy consumption. The recent decline in coal mining-driven CO
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emissions is mainly due to the strict deployment of coal mine gas and the changing structure of coal mines. The results from the scenarios suggest that the carbon emissions reduction potential will largely be determined by technology innovation in the coal mine gas industry. Policy implications for further addressing carbon emissions from the supply side of the coal industry include improvements in energy efficiency and coal mine gas extraction and utilization.
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