The near-instantaneous radial velocity of a target can be obtained using the Doppler effect of SAR, which is widely used in ocean current retrieval. However, in sea ice drift velocity measurements, ...only a Doppler centroid estimation algorithm in frequency domain has been studied, so whether there is a better algorithm is worth exploring. In this study, based on Sentinel-1A IW data, three Doppler centroid estimation algorithms applied to ocean current retrieval are selected. Combined with the characteristics of the TOPS mode, made two applicability adjustments to each algorithm, and finally applied the three algorithms to sea ice radial surface velocity measurements. The first adjustment is to explore and determine the optimal parameters. The second adjustment is to use parallel computing to improve the efficiency, which is improved by an average of 43.55%. In addition, the deviation of Doppler centroid estimation bias correction is verified using rainforest data, and the deviation is controlled at approximately 3 Hz. Based on the three algorithms, five sets of experiments are carried out in this study. By analyzing and comparing the results of each algorithm, it is found that the results of the three algorithms are relatively consistent, among which the correlation Doppler estimation algorithm has the advantages of high efficiency and high precision and is the most suitable method for sea ice drift measurement among the three methods. However, for SAR images with abnormal speckles caused by human activities, the sign Doppler estimation algorithm can effectively remove abnormal speckles and ensure the smoothness of the image with better adaptability.
The China-France Oceanography Satellite SCATterometer (CSCAT) can observe radar backscatter values on the same sea surface at multiple incidence angles, and is often used to estimate the ocean ...near-surface wind. However, CSCAT utilizes a novel scanning mechanism and the wind vector cell has a spatial resolution is 25 km or 12.5 km, which limit the study of high-resolution land and sea ice monitoring. To address this issue, this paper constructs a geometric model of the main lobe-to-ground projection relationship and generates the enhanced-resolution radar images. CSCAT data are applied to three main image reconstruction algorithms (SIR, AART, and MART), and experiments are performed in the Iceland and Hudson Bay, and verified by Sentinel-2 optical remote sensing data. The experiments show the geometric model for CSCAT improves the spatial resolution from traditional 25 km to 5 km, and the SIR-reconstructed images are characterized by higher accuracy and better suppression of noise than are those obtained with the AART and MART methods. Therefore, this study extends the application of domestic remote sensors and provides data support for high-resolution applications, such as land and sea ice monitoring.
To evaluate the therapeutic efficacy and safety of JAK inhibitor abrocitinib in patients with localized granuloma annulare (GA) and to review the available cases documented in English.
We presented a ...patient who had a persistent, localized granuloma anulare (GA) for one year and did not respond to traditional therapies. This patient was treated with oral abrocitinib at a dosage of 150 mg daily.
After 6 weeks of treatment with abrocitinib, the patient exhibited notable symptom improvement with no new lesions. No adverse events or recurrences were reported during the 5-month follow-up period.
Abrocitinib may be a promising and safe treatment option for patients with localized GA who do not respond to traditional therapies.
Sub-bottom profile data have the potential to characterize sediment properties but are seldom used for offshore site investigations because of uncertainties in rock-physics models. Deep-learning ...techniques appear to be poised to play very important roles in our processing flows for the interpretation of geophysical data. In this paper, a novel deep learning-based method for this task is proposed in which a nonlinear mapping between the observed data and sediment types is learned using a multi-attribute temporal convolution network (MATCN). Firstly, empirical mode decomposition (EMD) is employed for the original data, and intrinsic mode functions (IMFs) with multiple time scales are generated. Based on different IMFs, instantaneous frequency (IF) data under different IMFs can be obtained, while instantaneous phase (IP) and instantaneous amplitude (IA) data are obtained based on the original data. IF, IA and IP data are called attribute data, and are highly related to the attenuation, reflection, and interior structure of the sediment. Thus, IA, IF, and IP are used as the inputs, and a 1D convolutional neural network (CNN) and a time convolution network (TCN) are used to extract sequential features. Different feature representations are then fused. Combining cross-entropy loss function and class-edge loss function, the network is encouraged to produce classified results with more continuous sediment distributions compared with the traditional loss function. The real-data experiments demonstrate that the proposed MATCN has achieved good performance with an F measure greater than 70% in all cases, and greater than 80% in most cases.
In this paper, a novel method of pressurized metallurgy technology was proposed to improve cast structure of M42 high speed steel (HSS). The effect of solidification pressure (0.1, 1 and 2 MPa) on ...the cast structure of M42 HSS was investigated by means of experimental analysis and calculation of Thermo-Calc and DICTRA software. Increasing solidification pressure can obviously enhance cooling rate by improving interfacial heat transfer coefficient, which results in some remarkable improvement of the cast structure of M42 HSS. Firstly, the primary/secondary dendrite arm spacing and the average thickness of eutectic ledeburite reduce, which means dendrite structure is refined and eutectic ledeburite more homogeneously distributes with smaller size. Secondly, increasing solidification pressure, the volume fraction of M6C carbides decreases obviously and that of M2C increases correspondingly. And the morphology of M2C carbide changes from larger size lamellar and straight-rod shape into smaller size curved-rod morphology under higher solidification pressure due to larger nucleation number and overgrowth of γ, indicating that carbides are refined and distribute more uniformly. At last, higher solidification pressure is beneficial to reduce the lamellar spacing of M2C carbide and make compositions distribute more homogeneously.
Due to the increase in bacterial resistance, improving the anti-infectious immunity of the host is rapidly becoming a new strategy for the prevention and treatment of bacterial pneumonia. However, ...the specific lung immune responses and key immune cell subsets involved in bacterial infection are obscure. Actinobacillus pleuropneumoniae (APP) can cause porcine pleuropneumonia, a highly contagious respiratory disease that has caused severe economic losses in the swine industry. Here, using high-dimensional mass cytometry, the major immune cell repertoire in the lungs of mice with APP infection was profiled. Various phenotypically distinct neutrophil subsets and Ly-6C
inflammatory monocytes/macrophages accumulated post-infection. Moreover, a linear differentiation trajectory from inactivated to activated to apoptotic neutrophils corresponded with the stages of uninfected, onset, and recovery of APP infection. CD14
neutrophils, which mainly increased in number during the recovery stage of infection, were revealed to have a stronger ability to produce cytokines, especially IL-10 and IL-21, than their CD14
counterparts. Importantly, MHC-II
neutrophils with antigen-presenting cell features were identified, and their numbers increased in the lung after APP infection. Similar results were further confirmed in the lungs of piglets infected with APP and Klebsiella pneumoniae infection by using a single-cell RNA-seq technique. Additionally, a correlation analysis between cluster composition and the infection process yielded a dynamic and temporally associated immune landscape where key immune clusters, including previously unrecognized ones, marked various stages of infection. Thus, these results reveal the characteristics of key neutrophil clusters and provide a detailed understanding of the immune response to bacterial pneumonia.
Ribosomal protein SA (RPSA) of human brain microvascular endothelial cells (HBMECs) can transfer from the cytosol to the cell surface and act as a receptor for some pathogens, including Streptococcus ...suis serotype 2 (SS2), a zoonotic pathogen causing meningitis in pigs and humans. We previously reported that SS2 virulence factor enolase (ENO) binds to RPSA on the cell surface of HBMECs and induces apoptosis. However, the mechanism that activates RPSA translocation to the cell surface and induces ENO-mediated HBMEC apoptosis is unclear.
Here, we show that RPSA localization and condensation on the host cell surface depend on its internally disordered region (IDR). ENO binds to the IDR of RPSA and promotes its interaction with RPSA and vimentin (VIM), which is significantly suppressed after 1,6-Hexanediol (1,6-Hex, a widely used tool to disrupt phase separation) treatment, indicating that ENO incorporation and thus the concentration of RPSA/VIM complexes via co-condensation. Furthermore, increasing intracellular calcium ions (Ca
) in response to SS2 infection further facilitates the liquid-like condensation of RPSA and aggravates ENO-induced HBMEC cell apoptosis.
Together, our study provides a previously underappreciated molecular mechanism illuminating that ENO-induced RPSA condensation activates the migration of RPSA to the bacterial cell surface and stimulates SS2-infected HBMEC death and, potentially, disease progression. This study offers a fresh avenue for investigation into the mechanism by which other harmful bacteria infect hosts via cell surfaces' RPSA.
The effect of solidification pressure (0.5, 0.85 and 1.2 MPa) on heat transfer between ingot and mould was investigated with the measurement of cooling curves and calculation of heat transfer ...coefficient. Combined with cooling rate, temperature gradient and local solidification time (LST), the influence of pressure on solidification structure of 19Cr14Mn0.9N was revealed by macrostructure observation. The calculation results of heat transfer coefficient, obtained by the Beck-Nonlinear estimation technique, indicate that increasing solidification pressure obviously enhances heat transfer at the ingot/mould interface. And higher solidification pressure is benefit to increase cooling rate and temperature gradient of ingot. Meanwhile, increasing solidification pressure considerably suppresses nitrogen gas pore, and reduces the whole area of dispersing porosity and shrinkage, which is favorable to obtain a sound ingot. With the solidification pressure increasing from 0.5 to 1.2 MPa, the columnar zone is lengthened, the columnar-to-equiaxed transition (CET) position gradually moves to the ingot center, and both dendritic arm spacing (λ1 and λ2) and local solidification time (LST) gradually decrease. The solidification structure is significantly refined and compressed under higher solidification pressure.
The precise characterization and measurement of new nanomaterials and nano devices require in situ SEM nanorobotic instrumentation systems, which put forward further technical requirements on ...nanopositioning techniques of compact structure, cross-scale, nanometer accuracy, high vacuum and non-magnetic environment compatibility, etc. In this work, a novel cross-scale nanopositioning stage was proposed, which combined the advantages of piezoelectric stick-slip positioner and piezoelectric scanner techniques and adopted the idea of macro/micro positioning. A new structure design of a single flexible hinge shared by a small and large PZT was proposed to effectively reduce the size of the positioning stage and achieve millimeter stroke and nanometer motion positioning accuracy. Then, the cross-scale motion generation mechanism of the dual piezoelectric stick-slip drive was studied, the system-level dynamics model of the proposed positioning stages was constructed, and the mechanism design was optimized. Further, a prototype was manufactured and a series of experiments were carried out to test the performance of the stage. The results show that the proposed positioning stage has a maximum motion range of 20 mm and minimum step length of 70 nm under the small piezoceramic ceramic macro-motion stepping mode, and a maximum scanning range of 4.9 μm and motion resolution of 16 nm under the large piezoceramic ceramic micro-motion scanning mode. Moreover, the proposed stage has a compact structure size of 30 × 17 × 8 mm3, with a maximum motion speed of 10 mm/s and maximum load of 2 kg. The experimental results confirm the feasibility of the proposed stage, and nanometer positioning resolution, high accuracy, high speed, and a large travel range were achieved, which demonstrates that the proposed stage has significant performance and potential for many in situ SEM nanorobotic instrument systems.
(
) is an important opportunistic and zoonotic pathogen which is associated with many diseases in humans and animals. However, the pathogenicity of
has been neglected and the prevalence of
is poorly ...studied due to the lack of rapid and sensitive diagnosis techniques.
In this study, we infected mice and pigs with
strain from a human patient. An indirect ELISA was established using the KHE protein as the coating protein for the detection of
specific antibody in clinical samples. A nested PCR method to detect nuclei acids of
was also developed.
We showed that infection with
strain from a human patient led to mild lung injury of pigs. For the ELISA, the optimal coating concentration of KHE protein was 10 µg/mL. The optimal dilutions of serum samples and secondary antibody were 1:100 and 1:2500, respectively. The analytical sensitivity was 1:800, with no cross-reaction between the coated antigen and porcine serum positive for antibodies against other bacteria. The intra-assay and inter-assay reproducibility coefficients of variation are less than 10%. Detection of 920 clinical porcine serum samples revealed a high
infection rate by established indirect ELISA (27.28%) and nested PCR (19.13%). Moreover, correlation analysis demonstrated infection rate is positively correlated with gross population, Gross Domestic Product (GDP), and domestic tourists.
In conclusion,
is highly prevalent among pigs in China. Our study highlights the role of
in pig health, which provides a reference for the prevention and control of diseases associated with
.
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