Pancreatic cancer is an aggressive cancer of the digestive system, which is becoming a serious health problem worldwide. Overall survival for patients with pancreatic cancer is poor, mainly due to a ...lack of biomarkers to enable early diagnosis and a lack of prognostic markers that can inform decision-making, facilitating personalized treatment and an optimal clinical outcome. ncRNAs play an important role in pancreatic carcinogenesis. Here we review the literature on the role of ncRNAs as biomarkers in pancreatic cancer. We focus on the significance of ncRNAs as markers for early diagnosis, as prognostic biomarkers able to inform clinical management and as targets for novel therapeutics for patients with pancreatic cancer.
This paper presents two 4-bit (16-phase) mm-wave vector modulator phase shifters exploiting a novel in-phase and quadrature signal generator that consists of a single-input double-output cascode ...amplifier incorporating a lumped-element coupled-line quadrature coupler. The two circuit implementations have been designed and fabricated in a 28 nm fully depleted silicon-on-insulator CMOS. The first (PS1) achieves a higher gain and the second (PS2) has a more compact area (reduced to about 50%). Each consumes 18 mA from a 1.2 V supply. PS1 exhibits an average gain of 2.3 dB at 87.4 GHz and B 3dB from 78.8 to 92.8 GHz; rms gain error of 1.68 dB at 87.4 GHz and <;2 dB in the B 3dB ; rms phase error of 9.4° at 87.4 GHz and <;11.9° in B 3dB ; S 11 <; -10.5 dB in B 3dB ; average P 1dB of -7 dBm; and average noise figure (NF) equal to 10.8 dB at 87 GHz. PS2 exhibits an average gain of 0.83 dB at 89.2 GHz and B 3dB from 80.2 to 96.8 GHz; rms gain error of 1.46 dB at 89.2 GHz and <;2 dB in B 3dB ; rms phase error of 11.2° at 89.2 GHz and <;11.9° in B 3dB ; S 11 <; -11.5 dB in B 3dB ; average P 1dB of -6 dBm; and average NF of 11.9 dB at 89 GHz.
FOLFIRINOX, a combination of chemotherapy drugs (Fluorouracil, Oxaliplatin, Irinotecan -FOI), provides the best clinical benefit in pancreatic ductal adenocarcinoma (PDAC) patients. In this study we ...explore the role of miRNAs (MIR) as modulators of chemosensitivity to identify potential biomarkers of response. We find that 41 and 84 microRNA inhibitors enhance the sensitivity of Capan1 and MiaPaCa2 PDAC cells respectively. These include a MIR1307-inhibitor that we validate in further PDAC cell lines. Chemotherapy-induced apoptosis and DNA damage accumulation are higher in MIR1307 knock-out (MIR1307KO) versus control PDAC cells, while re-expression of MIR1307 in MIR1307KO cells rescues these effects. We identify binding of MIR1307 to CLIC5 mRNA through covalent ligation of endogenous Argonaute-bound RNAs cross-linking immunoprecipitation assay. We validate these findings in an in vivo model with MIR1307 disruption. In a pilot cohort of PDAC patients undergoing FOLFIRONX chemotherapy, circulating MIR1307 correlates with clinical outcome.
Advancements in stem cell technology together with an improved understanding of
organogenesis have enabled new routes that exploit cell-autonomous self-organization responses of adult stem cells ...(ASCs) and homogenous pluripotent stem cells (PSCs) to grow complex, three-dimensional (3D), mini-organ like structures on demand, the so-called organoids. Conventional optical and electrical neurophysiological techniques to acquire functional data from brain organoids, however, are not adequate for chronic recordings of neural activity from these model systems, and are not ideal approaches for throughput screenings applied to drug discovery. To overcome these issues, new emerging approaches aim at fusing sensing mechanisms and/or actuating artificial devices within organoids. Here we introduce and develop the concept of the
(LIO) technology for in-tissue sensing and actuation within 3D cell aggregates. This challenging technology grounds on the self-aggregation of brain cells and on integrated bioelectronic micro-scale devices to provide an advanced tool for generating 3D biological brain models with in-tissue artificial functionalities adapted for routine, label-free functional measurements and for assay's development. We complete previously reported results on the implementation of the integrated self-standing wireless silicon micro-devices with experiments aiming at investigating the impact on neuronal spheroids of sinusoidal electro-magnetic fields as those required for wireless power and data transmission. Finally, we discuss the technology headway and future perspectives.
Aberrant changes in microRNA expression contribute to lymphomagenesis. Bromodomain and extra-terminal domain inhibitors such as OTX015 (MK-8628, birabresib) have demonstrated preclinical and clinical ...activity in hematologic tumors. MicroRNA profiling of diffuse large B-cell lymphoma cells treated with OTX015 revealed changes in the expression levels of a limited number of microRNAs, including miR-92a-1-5p, miR-21-3p, miR-155-5p and miR-96-5p. Analysis of publicly available chromatin immunoprecipitation sequencing data of diffuse large B-cell lymphoma cells treated with bromodomain and extra-terminal domain (BET) inhibitors showed that the BET family member BRD4 bound to the upstream regulatory regions of multiple microRNA genes and that this binding decreased following BET inhibition. Alignment of our microRNA profiling data with the BRD4 chromatin immunoprecipitation sequencing data revealed that microRNAs downregulated by OTX015 also exhibited reduced BRD4 binding in their promoter regions following treatment with another bromodomain and extra-terminal domain inhibitor, JQ1, indicating that BRD4 contributes directly to microRNA expression in lymphoma. Treatment with bromodomain and extra-terminal domain inhibitors also decreased the expression of the arginine methyltransferase PRMT5, which plays a crucial role in B-cell transformation and negatively modulates the transcription of miR-96-5p. The data presented here indicate that in addition to previously observed effects on the expression of coding genes, bromodomain and extra-terminal domain inhibitors also modulate the expression of microRNAs involved in lymphomagenesis.
This paper reports comparative analyses of phase noise in Hartley, Colpitts, and common-source cross-coupled differential pair LC oscillator topologies in 28 nm CMOS technology. The impulse ...sensitivity function is used to carry out both qualitative and quantitative analyses of the phase noise exhibited by each circuit component in each circuit topology with oscillation frequency ranging from 1 to 100 GHz. The comparative analyses show the existence of four distinct frequency regions in which the three oscillator topologies rank unevenly in terms of best phase noise performance, due to the combined effects of device noise and circuit node sensitivity.
0.52-mW 30-GHz LNA in 22-nm FDSOI CMOS Spasaro, Michele; Zito, Domenico
IEEE transactions on circuits and systems. II, Express briefs,
10/2023, Letnik:
70, Številka:
10
Journal Article
Recenzirano
Odprti dostop
This brief reports a novel low-power 30GHz LNA in 22nm FDSOI CMOS technology. The LNA is based on an active network with CMOS inverting amplifier stages, and input and output feedforward impedance ...matching networks. It exhibits a peak gain of 11.4 dB, a noise figure of 5.8 dB, with a record power consumption of 0.52 mW from a 0.8 V supply. Owing to its circuit topology, the LNA is compact in size and its core area amounts to Formula Omitted mm2.
0.52mW 30GHz LNA in 22nm FDSOI CMOS Spasaro, Michele; Zito, Domenico
IEEE transactions on circuits and systems. II, Express briefs,
06/2023
Journal Article
Recenzirano
Odprti dostop
This brief reports a novel low-power 30GHz LNA in 22nm FDSOI CMOS technology. The LNA is based on an active network with CMOS inverting amplifier stages, and input and output feedforward impedance ...matching networks. It exhibits a peak gain of 11.4 dB, a noise figure of 5.8 dB, with a record power consumption of 0.52 mW from a 0.8 V supply. Owing to its circuit topology, the LNA is compact in size and its core area amounts to 0.20 × 0.22 mm2.
32 dB Gain 28 nm Bulk CMOS W-Band LNA Pepe, Domenico; Zito, Domenico
IEEE microwave and wireless components letters,
01/2015, Letnik:
25, Številka:
1
Journal Article
Recenzirano
A high gain W-band low noise amplifier for radiometric applications in 28 nm bulk CMOS technology is presented. Pads, inductors, capacitors and coplanar waveguides have been custom designed. The ...parasitic effects of the transistor layout have been evaluated by means of electromagnetic simulations and calculations based on data reported in the design rule manual of the technology. The amplifier consists of six cascode stages with input, output and interstage conjugate matching for maximum power transfer. Measurement results show a peak gain of 32 dB and a noise figure of 5.3 dB at 91 GHz.
This paper reports the results of the on-body experimental tests of a set of four planar differential antennas, originated by design variations of radiating elements with the same shape and ...characterized by the potential for covering wide and narrow bands. All the antenna designs have been implemented on low-cost FR4 substrate and characterized experimentally through on-body measurements. The results show the impact of the proximity to the human body on antenna performance and the opportunities in terms of potential coverage of wide and narrow bands for future ad hoc designs and implementations through wearable substrates targeting on-body and off-body communication and sensing applications.
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