Epigenetic changes are considered to be a frequent event during tumor development. Various methylation changes have been identified and show promise as potential cancer biomarkers. The aim of this ...study was to investigate promoter methylation of GATA4 and TP53 genes in endometrioid carcinoma of endometrium.
To search for promoter methylation of GATA4 and TP53 genes we used methylation-specific PCR (MSP) to compare the methylation status of 54 patients with endometrioid carcinoma of endometrium and 18 patients with normal endometrial tissue.
In our study MSP revealed GATA4 promoter methylation in 44 of 54 in the carcinoma group (81.5%), and in none of the control group. No methylation was observed in TP53 gene.
In conclusion, our study showed that there is significantly higher methylation in GATA4 gene in the endometrial cancer group compared with samples of non-neoplastic endometrium. The finding suggests the importance of hypermethylation of this gene in endometrial carcinogenesis and could have implications for future diagnostic and therapeutic strategies for endometrial cancer based on epigenetic changes.
Quinacrine is a drug that is known to heal neuronal cell culture infected with prions, which are the causative agents of neurodegenerative diseases called transmissible spongiform encephalopathies. ...However, the drug fails when it is applied in vivo. In this work, we analyzed the reason for this failure. The drug was suggested to “covalently” modify the prion protein via an acridinyl exchange reaction. To investigate this hypothesis more closely, the acridine moiety of quinacrine was covalently attached to the thiol groups of cysteines belonging to prion-derived peptides and to the full-length prion protein. The labeled compounds were conveniently monitored by fluorescence and absorption spectroscopy in the ultraviolet and visible spectral regions. The acridine moiety demonstrated characteristic UV–vis spectrum, depending on the substituent at the C-9 position of the acridine ring. These results confirm that quinacrine almost exclusively reacts with the thiol groups present in proteins and peptides. The chemical reaction alters the prion properties and increases the concentration of the acridine moiety in the prion protein.
The milk fat is unique among animal kinds of fat, because of its high content of fatty acids with short and medium long strings. Milk industry should be able to produce milk and dairy products with ...raising part of unsaturated FAs, which is useful and desirable for consumer. The milk samples were withdrawal individually once a month from 20 dairy cows of Holstein breading. The milk fat was separated by extraction in petrol-eter and further on it was re- esterifasised and methyl ester fatty acids were analysed by the help of gas chromatography (GLC). In exception of the current fatty acids was monitored also the substitution of families of fatty acids, it means saturated (SAFA), mono-non-saturated (MUFA) and poly-non-saturated (PUFA) greasy acids. From pasture growth is assessed the percentage of nitrogen matters (NL) according to Kjeldahla, crude fibre (CF), acidodetergent fibre (ADF) and neutral- detergent fibre (NDF) on instrument called Ankom Technology. The content of nitrogen matters in the herbal growth decreases during the herdsmen season and at the same time the content of CF increases. The content of coarse fibre during the herdsmen season in herbal growth increases from 18,72 % in May to 28,54 % in July and afterwards again diminishes to 20,51 % in September. It was stated the significant differences in funds ADF between the begin and the end of the pasture period (PMilk performance during the pasture period showed decreasing tendency in daily vessel from 23,4 l/piece/day in July to 22,10 l/piece/day in September. The content of oil in milk (as well as content of CF in pasture growth) during the pasture increases, namely from 3,78 % in May to 3,82 % in September. During the pasture season was the average efficiency of a dairy cow per day 22,44 l and during pasture period 20,27 l milks. The average concentration of fat in milk was higher during the winter period, up to 4,11 %, compared with 3,78 % in month September
Epigenetic changes are considered to be a frequent event during tumor development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation of tumor suppressor ...genes, DNA repair genes, cell cycle regulators and transcription factors. The aim of this study was to investigate promoter methylation of specific genes in endometrial cancer by comparison with normal endometrial tissue.
We used MS-MLPA (Methylation-specific Multiplex ligation-dependent probe amplification) to compare the methylation status of 59 tissue samples of endometroid type of endometrial carcinoma with 20 control samples of non-neoplastic endometrium.
Using 15% cut-off for methylation, we observed significantly higher methylation in the CDH13 gene in endometrial cancer group. We observed significantly higher methylation in both WT1 and GATA5 genes in IB stage of endometroid carcinoma. We also observed significantly higher methylation in GATA5 gene in the group of poorly differentiated endometroid carcinoma.
The findings suggest the importance of hypermethylation of CDH13, WT1 and GATA5 genes in endometrial carcinogenesis and could have implications for future diagnostic and therapeutic strategies of endometrial cancer based on epigenetic changes.
BACKGROUND: Recent transmissions of variant Creutzfeldt‐Jakob disease by blood transfusion emphasize the need for the development of prion screening tests. The detection of prions in blood is ...complicated by the presence of poorly characterized cellular prion protein (PrPC) in both plasma and blood cells. According to published studies, most of PrPC in blood cells resides in platelets (PLTs) and white blood cells.
STUDY DESIGN AND METHODS: To clarify conflicting reports about the quantity of PrPC associated with human red blood cells (RBCs), quantitative flow cytometry, Western blot (WB), and enzyme‐linked immunosorbent assay (ELISA) were used to measure protein levels in healthy donors.
RESULTS: RBCs expressed 290 ± 140 molecules of PrPC per cell, assuming equimolar binding of monoclonal antibody (MoAb) 6H4 to PrPC. Binding of alternate PrPC MoAbs, FH11 and 3F4, was substantially lower. WB estimated the level of PrPC per cell on RBCs to be just four times lower than in PLTs. A similar level of PrPC was detected using ELISA. The weak binding of commonly used MoAb 3F4 was not caused by PrPC conformation, truncation, or glycosylation, suggesting a covalent modification, likely glycation, of the 3F4 epitope.
CONCLUSIONS: Taken together, human RBCs express low but significant amounts of PrPC/cell, which makes them, due to high RBC numbers, major contributors to the pool of cell‐associated PrPC in blood. Previous reports utilizing MoAb 3F4 may have underestimated the amount of PrPC in RBCs. Likewise, screening tests for the presence of the abnormal prion protein in blood may be difficult if the abnormal protein is modified similar to RBC PrPC.
Effect of caseinomacropeptide concentrate addition on the growth of bifidobacteria Cicvarek, J.,Vyzkumny Ustav Mlekarensky, Prague (Czech Republic); Curda, L.,Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Technologie Mleka a Tuku; Elich, O.,MILCOM, Prague (Czech Republic) ...
Czech Journal of Food Sciences,
01/2010, Letnik:
28, Številka:
6
Journal Article
Recenzirano
Odprti dostop
We investigated the effect of caseinomacropeptide concentrate (CMP) on the growth and metabolic activity of Bifidobacterium bifidum CCDM 94 and Bifidobacterium lactis BB12 in skim milk with the ...addition of glucose and of various combinations of yeast extract, L-cysteine-HCl, ascorbic acid, and CMP. The microorganisms grew well on the medium containing a combination of all the nutrients mentioned, and on that where only L-cysteine-HCl was missing. In the media enhanced with CMP a shorter lag-phase occurred than in those without CMP. The shortest lag-phase and the longest log-phase with a high growth rate were observed in media No. 2 (complete medium with CMP) and No. 8 (complete medium without L-cysteine-HCl).
Evidence of the impact of in utero exposure to anti-tumor necrosis factor (TNF)-alpha on long-term childhood development is limited. The aim was to assess the impact of in utero exposure to ...anti-TNF-alpha due to mothers' inflammatory bowel disease (IBD) on long-term postnatal development of exposed children.
We included consecutive children (≥12 months of age) born to mothers with IBD (2007-2016) treated with anti-TNF-alpha during pregnancy in 3 centers in the Czech Republic. A control group was comprised of unexposed children of non-IBD mothers undergoing mandatory check-ups at general pediatricians' offices. Data on perinatal period, psychomotor development, vaccination, infections, antibiotics, and allergy were collected by treating pediatricians using a predefined questionnaire.
Seventy-two exposed and 69 unexposed children were included (median age, 35 and 50 months, respectively). Exposed children had growth and psychomotor development similar to controls. There was no significant difference in infectious complications within the first year of life (23.9% vs 17.4%; P = 0.36) or during the whole follow-up between exposed infants and controls (P = 0.32). Concomitant immunosuppressants during pregnancy and anti-TNF-alpha levels in cord blood were not associated with elevated infection rate within the first year of life (P > 0.05). Over 95% of exposed children had adequate serologic response to vaccination, except for haemophilus and mumps vaccines. Clinically manifested allergy was similar between the groups (P = 0.98).
Anti-TNF-alpha exposure in utero does not seem to have a negative impact on postnatal development of children with regard to infectious complications, allergy, growth, or psychomotor development when compared with unexposed children of non-IBD women.
V článku se věnujeme srovnání klasické a bayesovské exploratorní faktorové analýzy. Použili jsme náhodně generovaná testovací data s různými velikostmi vzorku, strukturou faktorových nábojů, škálami ...odpovědí a velikostmi náhodné chyby. Provedli jsme oba druhy exploratorní faktorové analýzy a na základě srovnání výsledků a celkové zkušenosti z provedených analýz můžeme uzavřít, že u klasické a bayesovské exploratorní faktorové analýzy není rozdíl v přesnosti odhadu faktorových nábojů. Bayesovský přístup poskytuje více informací, které jsou intuitivněji interpretovatelné, ale na druhou stranu námi použitý model má i nevýhody, jako je nutnost ruční úpravy zdrojových kódů a časová náročnost výpočtů. Bayesovský přístup je tak podle nás vhodnější spíše pro konfirmatorní faktorovou analýzu.
An unusual case of focal accumulation of sarcoid-like granulomas occurring within the thyroid gland of a 43-year-old female patient is reported. The granulomas were found solely at the site of ...previous fine needle aspiration biopsy. The follow-up did not show any symptoms of systemic granulomatous disease. The pathogenesis of this lesion is discussed.
Epigenetic aberrations are well known to play an important role in carcinogenesis, and also have a great potential to serve as biomarkers in many types of cancers, including ovarian cancer in which ...sensitive and specific biomarkers and detection methods are critically needed. The aim of this study was to investigate methylation of cadherin genes
CDH10
,
CDH13
and
CDH18
in ovarian cancer tissue by comparison with control tissue. The study group consisted of 38 patients with ovarian cancer and 25 control patients. For detection of epigenetic events we used next generation sequencing, the most important data were confirmed using high-resolution melting analysis and real-time PCR. We observed significantly higher methylation in
CDH13
, sporadic methylation in
CDH10
and loss of methylation in
CDH18
in the ovarian cancer group compared with the control group. These observations suggest that changes in methylation of cadherin genes may be one of the major mechanisms associated with ovarian cancer progression. In addition, because of the high frequency of methylation of the
CDH13
gene in the early stages of ovarian cancer, the analyzed CpG sites might be good targets for next study of potential ovarian cancer screening biomarkers.
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