High frame rate (HFR) echocardiography may be of benefit for functional analysis of the heart. In current clinical equipment, HFR is obtained using multi-line acquisition (MLA) which typically ...requires broadening of transmit beams. As this may result in a significant degradation of spatial resolution and signal-to-noise ratio (SNR), the capacity of MLA to obtain high quality HFR images remains limited. As an alternative, we have demonstrated by computer simulation that simultaneously transmitting multiple focused beams into different directions multi-line transmit (MLT), can increase the frame rate without significantly compromising the spatial resolution or SNR. This study aimed to experimentally verify these theoretical predictions both in vitro and in vivo to demonstrate, for the first time, that cardiac MLT imaging is feasible. Hereto, the ultrasound advanced open platform, equipped with a 2.0 MHz phased array, was programmed to interleave MLT and conventional single line transmit (SLT) beam forming. Using these two beam forming methods, images of phantoms and healthy volunteers were acquired and investigated both qualitatively and quantitatively. The results confirmed our simulations that image quality of a 4MLT imaging system with a Tukey apodization scheme is very competitive to that of SLT while providing a 4 times higher frame rate. It is also demonstrated that MLT can be combined with MLA to provide images at 12-to 16-fold frame rate (about 340-450 Hz) without significantly compromising spatial resolution and SNR. This is thus the first study to demonstrate that this new ultrasound imaging paradigm is viable which could have significant impact on future cardiac ultrasound systems.
Several ultrasound (US) methods have been recently proposed to produce 2-D velocity vector fields with high temporal and spatial resolution. However, the real-time implementation in US scanners is ...heavily hampered by the high calculation power required. In this work, we report a real-time vector Doppler imaging method which has been integrated in an open research system. The proposed approach exploits the plane waves transmitted from two sub-arrays of a linear probe to estimate the velocity vectors in 512 sample volumes aligned along the probe axis. The method has been tested for accuracy and reproducibility through simulations and in vitro experiments. Simulations over a 0° to 90° angle range of a 0.5 m/s peak parabolic flow have yielded 0.75° bias and 1.1° standard deviation for direction measurement, and 0.6 cm/s bias with 3.1% coefficient of variation for velocity assessment. In vitro tests have supported the simulation results. Preliminary measurements on the carotid artery of a volunteer have highlighted the real-time system capability of imaging complex flow configurations in an intuitive, easy, and quick way, as shown in a sample supplementary movie. These features have allowed reproducible peak velocity measurements to be obtained, as needed for quantitative investigations on patients.
Quantitative blood velocity measurements, as currently implemented in commercial ultrasound scanners, are based on pulsed-wave (PW) spectral Doppler and are limited to detect the axial component of ...the velocity in a single sample volume. On the other hand, vector Doppler methods produce angle-independent estimates by, e.g., combining the frequency shifts measured from different directions. Moreover, thanks to the transmission of plane waves, the investigation of a 2-D region is possible with high temporal resolution, but, unfortunately, the clinical use of these methods is hampered by the massive calculation power required for their real-time execution. In this paper, we present a novel approach based on the transmission of plane waves and the simultaneous reception of echoes from 16 distinct subapertures of a linear array probe, which produces eight lines distributed over a 2-D region. The method was implemented on the ULAO-OP 256 research scanner and tested both in phantom and in vivo. A continuous real-time refresh rate of 36 Hz was achieved in duplex combination with a standard B-mode at pulse repetition frequency of 8 kHz. Accuracies of -11% on velocity and of 2°on angle measurements have been obtained in phantom experiments. Accompanying movies show how the method improves the quantitative measurements of blood velocities and details the flow configurations in the carotid artery of a volunteer.
Although severely affected by the angle dependency, carotid artery peak systolic velocity measurements are widely used for assessment of stenosis. In this study, blood peak systolic velocities in the ...common and internal carotid arteries of both healthy volunteers and patients with internal carotid artery stenosis were measured by two vector Doppler (VD) methods and compared with measurements obtained with the conventional spectral Doppler approach. Although the two VD techniques were completely different (using the transmission of focused beams and plane waves, respectively), the measurement results indicate that these techniques are nearly equivalent. The peak systolic velocities measured in 22 healthy common carotid arteries by the two VD techniques were very close (according to Bland-Altman analysis, the average difference was 3.2%, with limits of agreement of ± 8.6%). Application of Bland-Altman analysis to comparison of either VD technique with the spectral Doppler method provided a 21%-25% average difference with ± 13%-15% limits of agreement. Analysis of the results obtained from 15 internal carotid arteries led to similar conclusions, indicating significant overestimation of peak systolic velocity with the spectral Doppler method. Inter- and intra-operator repeatability measurements performed in a group of 8 healthy volunteers provided equivalent results for all of the methods (coefficients of variability in the range 2.7%-6.9%), even though the sonographers were not familiar with the VD methods. The results of this study suggest that the introduction of vector Doppler methods in commercial machines may finally be considered mature and capable of overcoming the angle-dependent overestimation typical of the standard spectral Doppler approach.
Conventional ultrasound Doppler techniques estimate the blood velocity exclusively in the axial direction to produce the sonograms and color flow maps needed for diagnosis of cardiovascular diseases. ...In this paper, a novel method to produce bi-dimensional maps of 2-D velocity vectors is proposed. The region of interest (ROI) is illuminated by plane waves transmitted at the pulse repetition frequency (PRF) in a fixed direction. For each transmitted plane wave, the backscattered echoes are recombined offline to produce the radio-frequency image of the ROI. The local 2-D phase shifts between consecutive speckle images are efficiently estimated in the frequency domain, to produce vector maps up to 15 kHz PRF. Simulations and in vitro steady-flow experiments with different setup conditions have been conducted to thoroughly evaluate the method's performance. Bias is proved to be lower than 10% in most simulations and lower than 20% in experiments. Further simulations and in vivo experiments have been made to test the approach's feasibility in pulsatile flow conditions. It has been estimated that the computation of the frequency domain algorithm is more than 50 times faster than the computation of the reference 2-D cross-correlation algorithm.
Ultrasound vector Doppler techniques for three-dimensional (3-D) blood velocity measurements are currently limited by low temporal resolution and high computational cost. In this paper, an efficient ...3-D high-frame-rate vector Doppler method, which estimates the displacements in the frequency domain, is proposed. The novel method extends to 3-D an approach so far proposed for two-dimensional (2-D) velocity measurements by approximating the (x, y, z) displacement of a small volume through the displacements estimated for the 2-D regions parallel to the y and x directions, respectively. The new method was tested by simulation and experiments for a 3.7 MHz, 256-element, 2-D piezoelectric sparse spiral array. Simulations were also performed for an equivalent 7 MHz Capacitive Micromachined Ultrasonic Transducer spiral array. The results indicate performance (bias ± standard deviation: 6.5 ± 8.0) comparable to the performance obtained by using a linear array for 2-D velocity measurements. These results are particularly encouraging when considering that sparse arrays were used, which involve a lower signal-to-noise ratio and worse beam characteristics with respect to full 2-D arrays.
Cardiovascular diseases, the leading cause of death in the world, are often associated with the dysfunction of the left ventricle. Even if, in clinical practice, the myocardial function is often ...assessed through visual wall motion scoring on B-mode images, quantitative techniques have been introduced, e.g., ultrasound tissue Doppler imaging (TDI). However, this technique suffers from the limited frame rate of currently available imaging techniques that needs to be balanced with the field of view. High-frame-rate (HFR) cardiac imaging has been recently tested off-line by simultaneously transmitting multiple focused beams into different directions and acquiring raw channel data into a PC. Several image lines were then reconstructed from the echoes of each transmission (TX) event. The same approach has been used to increase the TDI frame rate without restricting the field of view. This paper demonstrates the real-time feasibility of multiline TX and acquisition methods for both HFR cardiac B-mode and TDI. These approaches have been implemented on the ULA-OP 256 research scanner, by taking care that the related resources were optimally exploited for these new applications. The obtainable performance in terms of image quality and frame rate has also been investigated. Experiments performed with a 128-element phased array probe show, for the first time, that real-time B-mode imaging is feasible at up to 1150 Hz without significant reduction in image quality or field of view. The implementation of a real-time TDI algorithm allowed obtaining TDI images with a frame rate of 288 Hz for a 90°-wide field of view. Finally, in vivo examples demonstrate the feasibility and the suitability of the method in clinical studies.
Multispectral photoacoustic imaging is a powerful noninvasive medical imaging technique that provides access to functional information. In this study, a set of methods is proposed and validated, with ...experimental multispectral photoacoustic images used to estimate the concentration of chromophores. The unmixing techniques used in this paper consist of two steps: (1) automatic extraction of the reference spectrum of each pure chromophore; and (2) abundance calculation of each pure chromophore from the estimated reference spectra. The compared strategies bring positivity and sum-to-one constraints, from the hyperspectral remote sensing field to multispectral photoacoustic, to evaluate chromophore concentration. Particularly, the study extracts the endmembers and compares the algorithms from the hyperspectral remote sensing domain and a dedicated algorithm for segmentation of multispectral photoacoustic data to this end. First, these strategies are tested with dilution and mixing of chromophores on colored 4% agar phantom data. Then, some preliminary in vivo experiments are performed. These consist of estimations of the oxygen saturation rate (sO2) in mouse tumors. This article proposes then a proof-of-concept of the interest to bring hyperspectral remote sensing algorithms to multispectral photoacoustic imaging for the estimation of chromophore concentration.
Due to the primary radiation force, microbubble displacement has been observed previously in the focal region of single-element and array ultrasound probes. This effect has been harnessed to increase ...the contact between the microbubbles and targeted endothelium for drug delivery and ultrasound molecular imaging. In this study, microbubble displacements associated with plane-wave (PW) transmission are thoroughly investigated and compared to those obtained in focused-wave (FW) transmission over a range of pulse repetition frequencies, burst lengths (BLs), peak negative pressures, and transmission frequencies. In PW mode, the displacements, depending upon the experimental conditions, are in some cases consistently higher (e.g., by 28%, when the longest BL was used at PRF = 4 kHz), and the axial displacements are spatially more uniform compared to FW mode. Statistical analysis on the measured displacements reveals a slightly different frequency dependence of statistical quantities compared to transient peak microbubble displacements, which may suggest the need to consider the size range within the tested microbubble population.
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