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Green inhibitors are an important way to decrease the corrosion rate of different industrial metals. The present work shows the corrosion behavior of the mild steel medium (MS) in ...hydrochloric acid 1 M by the aqueous extract of Artemisia Herba Alba (AHA), which is an green sustainable inhibitor to reduce its corrosive action by exploiting a variety of techniques and methods namely: Weight loss, electrochemical techniques electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization (PDP, SEM-EDX, XPS and theoretical calculations for the majority molecule. Hydrodistillation is the extraction method that has been used to prepare the aqueous extracts. Otherwise, phytochemical screening characterization of different parts of the plant was applied to show the proportions of chemical compounds that exist in the plants. The highest inhibition efficiency obtained is 92% for 0.4 g/L of AHA aqueous extract in 1 M HCl at 303 K. PDP study confirmed that the AHA extract is of mixed-kind inhibitor. A CPE, Q determined by fit and graphical methods plotted by synthetic data, was utilized. To characterize the mild steel surface, scanning electron microscopy was also used; SEM/EDX allowed the investigation of the AHA effect on the surface of mild steel specimens. To comprehend the adsorption mechanism of AHA extract for MS in the 1 M HCl, XPS technique was accomplished. Theoretical approaches based on chemical quantum calculations and molecular dynamics simulation clearly explains the mode of adsorption of the majority molecule on the iron surface.
•Two Artemisia (A. herba and A. drancunculus) essential oils were investigated.•The essential oils were chemically characterized by GC–MS technique.•The essential oils exhibited significant ...antioxidant and antimicrobial activities.•The species can be considered as valuable sources of bioactive agents in pharmaceutical and nutraceutical industries.
Artemisia herba alba and Artemisia dracunculus are widely used in folk medicine by many ethnic groups to relieve and treat various ailments, including diabetes, cancer, and infectious diseases. The current investigation aims to determine the phytochemical profile, antibacterial, anticandidal and antioxidant activities of Moroccan A. herba alba and A. dracunculus essential oils (EOs). Antioxidant activity was carried out using four complementary assays, namely ABTS and DPPH radical scavenging techniques, ferric-reducing power (FRAP) and lipid peroxidation tests. The antimicrobial potential of A. herba-alba and A. dracunculus EOs was examined against a variety of Gram-positive and Gram-negative bacteria, and yeasts, namely Staphylococcus aureus, Bacillus cereus Micrococcus luteus, Escherichia coli, Klebsiella aerogenes, Candida albicans and Candida tropicalis. The results demonstrated that the A. herba-alba and A. dracunculus EOs exhibited remarkable inhibitory effects against all tested microorganisms using disk-diffusion method and were competitor to the examined antibiotics, demonstrating a maximum inhibition diameter of the Gram-positive bacteria. The most susceptible was B. cereus (31.33 ± 4.04 mm), and the most susceptible Gram-negative bacteria was K. aerogenes (19.79 ± 1.53 mm), and C. tropicalis was the most susceptible Candida spp. to A. herba alba EOs (27.00 ± 0.66 mm). The MIC, MBC, and MFC values indicate that these EOs were highly effective at minimal concentrations. However, toxicological assessment, pharmacodynamics and pharmacokinetic investigations are necessary to confirm the safety and the beneficial effects of these oils in human healthcare and industrial applications as potential sources of natural antioxidants and preservative agents.
Essential oil from aerial parts of Artemisia herba-alba from Morocco was hydrodistilled and its chemical composition oil was investigated by capillary GC and GC/MS. The major components were ...1,8-cineole (35.6%) and camphor (24.1%). Artemisia herba-alba essential oil AHAO was tested as corrosion inhibitor of stainless steel (SS) in 1M H3PO4 using potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy measurements (EIS) and scanning electronically microscopy (SEM) studies. The results obtained showed that the essential oil of Artemisia reduces the corrosion rate. Tafel polarization method indicates that the plant extract behaves as a mixed type inhibitor. The inhibition efficiency increased with inhibitor concentration to attain 88% at 1g.L−1 of oil at 298K. Nyquist diagrams obtained from impedance studies provide results confirming the anti-corrosion effect of the studied plant. The temperature effect on the corrosion behavior of (SS) in 1M H3PO4 without and with AHAO at 1g.L−1 was studied in the temperature range from 298 to 353K. Thermodynamic parameters suggested that the adsorption is spontaneous and exothermic process and support physical adsorption mechanism. The experimental data fits well into the Langmuir adsorption isotherm model. SEM/EDS studies provide the confirmatory evidence for the protection of (SS) by the green inhibitor. The results obtained from these methods used are in good agreement.
This study aimed to compare the essential oil yield and chemical composition of Artemisia herba-alba in the Anti-Atlas region, considering different harvest dates. The antibacterial and antifungal ...properties were also evaluated. Essential oils yields were 1.87 %, 1.85 %, and 2.35 % for the April, June and October harvests, respectively. Chromatographic analysis identified 21 compounds, with camphor, 1,8-cineole, and camphene as the main constituents. Artemisia herba-alba essential oils exhibited significant antibacterial and antifungal activities. The October-harvested oil showed notable antibacterial effects against Staphylococcus epidermidis (20 mm), while the June-collected essential oil demonstrated strong activity with a 20.66 mm inhibition zone against Acinobacter baumannii. Furthermore, the June-collected oil displayed significant antifungal activity with a 70.58 % inhibition index against Fusarium culmorum. Despite varying reducing powers with IC50 values, the antioxidant activity of the essential oils was relatively weaker compared to l-ascorbic acid, possibly due to the prevalence of non-phenolic compounds.
Ed-Dra A, Filali FR, Presti VL, Zekkori B, Nalbone L, Elsharkawy ER, Bentayeb A, Giarrtana F. 2021. Effectiveness of essential oil from the Artemisia herba-alba aerial parts against ...multidrug-resistant bacteria isolated from food and hospitalized patients. Biodiversitas 22: 2995-3005. The World Health Organization has sounded the warning on the diffusion of multidrug resistance (MDR) bacteria, requiring solutions and alternatives to solve the therapeutic failure that may occur. This study aims to evaluate the antioxidant activity and bactericidal effectiveness against MDR bacteria of Artemisia herba-alba essential oil (A-EO) collected from semi-arid region of Morocco. Chemical composition of the A-EO was determined by Gas Chromatography-Flame Ionisation Detector and Gas Chromatography-Mass Spectrometry, while the antioxidant activity was performed by DPPH scavenging activity and ?-carotene bleaching assay. Antibacterial activity of A-EO, performed by disc diffusion assay and broth dilution method, was tested against: four MDR strains (Escherichia coli, Staphylococcus aureus, Salmonella Typhimurium and Enterococcus faecalis) isolated from food matrices, two (Klebsiella pneumonia and Pseudomonas aeruginosa) from hospitalized patients, and Escherichia coli ATCC 25922 as reference strain. Davanone was the main compound among the 17 identified. An antioxidant activity with IC50 of 1.13±0.02 mg/mL, EC50 of 2.12±0.05 mg/mL and RC50 of 0.87±0.02 mg/mL was observed. A weak activity against P. aeruginosa was observed, while it was intermediate or high against the other bacteria. This study confirms that A-EO could be a suitable alternative to antibiotics in the infection treatment related to MDR bacteria.
Aims: Plant communities growing along gypsum hills in NE Spain show a distinct zonation that may depend on water table depth, salt and nutrient availability gradients between hilltops and saline ...depressions. We analysed the main sources of water used by woody species in these communities. Specifically, we assessed whether hydrological niche segregation occurred among dominant woody species with different root depth and plant location along gypsum hills during spring and summer. Location: Miocene gypsum formations of NE Spain, Middle-Ebro depression. Methods: We analysed the oxygen and hydrogen isotope composition of the xylem water of ten dominant woody species growing along gypsum hills and compared it to water extracted from the top 20 cm of the soil, gypsum crystallization water and groundwater, both in spring and summer. Bayesian isotope mixing models were used to estimate the proportion of each water source used by different species. Results: There was a clear segregation in the sources of water used by dominant woody species from plant communities co-existing on gypsum hills, and such segregation responded to their rooting depth and position along the hill. Shallow-rooted plants growing out of saline depressions exploited superficial free soil water and gypsum crystallization water, and had limited access to groundwater, particularly during summer. In contrast, deep-rooted plants and shallow-rooted plants from saline depressions thrived preferentially on the water table. Such differences were noticeable in spring, but became sharper during summer. Conclusions: Woody species from gypsum hills have different hydrological niches. Niche segregation is mainly related to the depth of the root system of plants and to their position along gypsum hills, which determine proximity to the water table. It is accentuated during summer, highlighting the role of summer drought as a structuring factor in gypsum plant communities. The segregation of the water sources used by plants is related to a suite of phenological, functional and anatomical features, indicating adaptation of plant species to their respective hydrological niches.
•Regular consumption of Artemisia herba-alba is advisable and beneficial for preventing inflammation-related diseases.•Extracts of Artemisia herba-alba have a great potential for medical and local ...applications, i.e. against skin sunburns.•The development of industrial usage of Artemisia herba-alba is an economical alternative for farmers in semi-desert regions, i.e. Algerian lands.
Artemisia herba-alba (AHA) is a medicinal plant known for its preventive capacities against chronic diseases. Inflammation is a naturally occurring cellular response to noxious stimuli, and both acute and chronic inflammation are involved in human disorders. Preventing and limiting the negative impact of inflammation by using phytocompounds has attracted a lot of attention. Here, we show that AHA extracts are potent anti-inflammatory products at low doses in white blood cells, treated with phorbol myristate acetate (PMA), a pro-inflammatory trigger. Regardless of the mode of extraction, i.e. maceration or microwave, all extracts reduced on dose-dependent manner reactive oxygen species (ROS) production induced by the pro-inflammatory compound PMA in white blood cells and leukemic Jurkat cell line. The induction of the anti-inflammatory effect was evaluated by the measurement of glutathione metabolism (glutathione reductase, transferase, and peroxidase), and protein-free thiols in Jurkat cells. Jurkat cells incubated with low to high concentrated AHA extracts, ranged from 10 to 100 g/mL, induced a fast cell response measured at one and six hours. Interestingly, extracts from the microwave showed a stronger induction, 5 to 10 fold changes at early time points, and maintained over 72 h. On the other hand, AHA extracts showed a moderate anti-proliferative effect in the leukemic Jurkat line. We concluded that AHA extracts enhance directly anti-inflammatory cell response, earlier via ROS induction. Later, however, vigorously maintained, independently from ROS, suggesting the intervention of the cellular metabolism in enhancing these effects. Considering the preventive and anti-inflammatory potential of AHA, regular consumption is advisable, and phytotherapeutic applications are encouraged to treat local inflammations.
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The aim of this study was to determine the phenolic compounds from Artemisia herba-alba Asso, in order to evaluate their antioxidant and antibacterial activities, in vitro. The extraction of phenolic ...compounds was carried out by the maceration technique using absolute ethanol, absolute methanol, and distilled water. The quantification of polyphenols and flavonoids was performed using the Folin-Ciocalteu reagent and the aluminum trichloride method, respectively. The evaluation of the antioxidant activity of the extracts was carried out by the FRAP, the DPPH· radical trapping, and the neutralization of the hydrogen peroxide technique. The lipid peroxidation was assessed by thiobarbituric acid reactive substances. In addition, the antibacterial activity of the three extracts was tested on Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 33862, Escherichia coli ATCC 2592, and Pseudomonas aeruginosa ATCC 27853 bacteria using agar diffusion and agar incorporation methods. The results showed that the methanolic extract was highly rich in polyphenols and flavonoids. Also, the reducing power CE50 = 249.88 ± 6.07 pg/ml and the inhibition capacity of the DPPH· radical CI50 = 34.71 ± 0.96 pg/ml were significantly higher (p<0.05) than the ethanolic and aqueous extracts. Also, a highly significant inhibitory potential of lipid peroxidation was obtained with the methanolic extract (MDA = 66.97 ± 3.61 pmol/g tissue). However, a highly significant hydrogen peroxide scavenging effect was obtained from the ethanolic extract. A better antibacterial activity was obtained with the methanolic and ethanolic extracts.
A total of 80 individuals collected from eight populations growing wild in different geographic zones were considered to assess the intraspecific variability of essential oil composition, genetic ...diversity and population structure of Artemisia herba-alba.
The essential oil composition varied significantly between populations. Essential oil profiles were classified into four chemotypes (trans-sabinyl acetate, α-thujone/trans-sabinyl acetate, camphor and α-thujone/camphor/β-thujone). Despite significant correlation between the amount of some essential oil compounds and a set of climatic data, the global chemical divergence among populations was not related to their bioclimatic and geographic appurtenances.
A high level of genetic diversity within populations was revealed either with RAPD and ISSR markers (Na = 1.67, PPL = 66.5%, H = 0.26, I = 0.38 and Na = 1.7, PPL = 69.8%, H = 0.26, I = 0.38, respectively). The level of genetic diversity varied across populations and chemotypes. Populations from the α-thujone/trans-sabinyl acetate chemotype exhibited the highest genetic diversity as revealed by the RAPD markers. However, populations from α-thujone/camphor/β-thujone chemotype showed the important genetic variation determined by ISSR markers. A significant genetic differentiation among populations and among chemotypes was detected.
The combined analysis showed a significant correlation (r = 0.484, p = .032) between the chemical and molecular markers. The PCA, performed on percentages of major oil compounds and the frequencies of polymorphic RAPD and ISSR bands, divided populations according to their chemotypic classification.
Taking into consideration the current situation of A. herba-alba populations and their endangered habitats, these results are of value in order to ensure the in-situ and ex-situ conservation of this medicinal species.
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•The essential oil composition varied significantly between populations.•Essential oil profiles were classified into four chemotypes.•A high level of genetic diversity was revealed with RAPD and ISSR markers.•A significant genetic differentiation among populations was detected.•A significant concordance between the genetic and the chemical data was detected.
In this study, the essential oils (EOs) of six Algerian plants (
L.,
Asso,
L.,
s L.,
L. and
Chaix) were obtained by hydrodistillation, and their compositions determined by GC-MS and GC-FID. The ...antioxidant activity of the EOS was evaluated via 2,2'-diphenyl-1-picrylhydrazyl (DPPH), ferric-reducing/antioxidant power (FRAP) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Moreover, their cytotoxic effect was evaluated-as well as their tyrosinase, acetyl- and butyryl-cholinesterase (AChE and BuChE) inhibitory activities. The chemical analyses detected 44, 45, 51, 53, 26 and 40 compounds in EOs of
,
,
,
,
and
, respectively.
EO was mainly composed of β-pinene (20.7%), while
EO contained davanone D (49.5%) as the main component. α-Pinene (41.8%) was detected as the major constituent in both
(41.8%) and
(37.8%) EOs.
EO was characterized by pulegone as the most abundant (76.9%) compound, while linalool (35.8%) was detected as a major constituent in
EO. The antioxidant power evaluation revealed IC50 values ranging from 2.61 to 91.25 mg/mL for DPPH scavenging activity, while the FRAP values ranged from 0.97-8.17 µmol Trolox equivalents (TX)/g sample. In the ABTS assay, the values ranged from 7.01 to 2.40 µmol TX/g sample. In the presence of 1 mg/mL of the samples, tyrosinase inhibition rates ranged from 11.35% to 39.65%, AChE inhibition rates ranged from 40.57% to 73.60% and BuChE inhibition rates ranged from 6.47% to 72.03%. A significant cytotoxic effect was found for
EO. The obtained results support some of the traditional uses of these species in food preservation and for protection against several diseases.
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