Essential oils (EOs) are one of the most important groups of plant metabolites responsible for their biological activities. This study was carried out to study the chemical composition and the ...antimicrobial effects of Artemisia herba-alba and Origanum majorana essential oils against some Gram-positive and Gram-negative bacteria, and a fungal strain isolated from spoiled butter. The plants were collected in the region Azzemour of South West Morocco and the EOs, extracted by hydrodistillation, were analyzed by GC-MS. The antimicrobial activity was determined using the agar paper disc method. The main components of A. herba-alba EO were cis-thujone, trans-thujone and vanillyl alcohol; in O. majorana EO terpinen-4-ol, isopulegol and β-phellandrene predominated. Both essential oils exhibited growth inhibiting activities in a concentration-dependent manner on several microorganism species. Our results demonstrated that O. majorana and A. herba-alba EOs could be effective natural antibacterial agents in foods.
The development of effective and eco-friendly insecticides is a demand. Green nanoparticles synthesis is an important field in pest control strategy, which has cost effective and faster than physical ...and chemical methods. In this study, silver nanoparticles (AgNPs) were synthesized using Artemisia herba-alba plant extract to be tested against an important pest of non-edible plants such as cotton. UV-vis spectrophotometry, TEM, XRD and FTIR were used to confirm the biophysical characterization of AgNP formation, which appeared as spherical particles with size ranging from 9.68 to 36.7 nm. Feeding and contact application methods were used to evaluate the larvicidal activity of green-synthesized AgNPs against the cotton pest Spodoptera littoralis larvae. LC
50
and LC
90
values of synthesized AgNPs were calculated. After 5 days of treatment, the calculated LC
50
of applied AgNPs by feeding application was 74.569 ml/ml (soln./ H
2
O), whereas contact application recorded LC
50
of 27.47 ml/ml after 30 h of treatment. Morphological and histopathological abnormalities after AgNPs treatment were detected as demelanization and mummified shape of larvae, the destruction of the cuticle layer, disintegration of gut epithelia and gonad deformation. Our results proved the success of green-synthesized NPs application against S. littoralis larva, which open new avenues in pest control.
The study of bioactive molecules of natural origin is a focus of current research. Thymus algeriensis and Artemisia herba-alba are two medicinal plants widely used by the Moroccan population in the ...traditional treatment of several pathologies linked to inflammation. This study aimed to evaluate the single and combined antioxidant, anti-inflammatory and analgesic effects of the essential oils extracted from these two medicinal plants, and also their potential toxicity. Essential oils were extracted using hydro-distillation in a Clevenger-type apparatus. The antioxidant activity was evaluated by two methods: the scavenging of the free radical DPPH, and the reduction in iron. Anti-inflammatory activity was evaluated by evaluating the edema development induced by carrageenan injecting, while the analgesic power was evaluated according to the number of abdominal contortions induced by the intraperitoneal injection of acetic acid (0.7%). The acute oral toxicity was performed to assess the potential toxicity of the studied EOs, followed by an analysis of the blood biochemical parameters. The results of the two antioxidant tests indicated that our extract mixture exhibits good iron reduction capacity and very interesting DPPH free radical scavenging power, with an IC50 of around 4.38 ± 0.98 μg/mL higher than that of the benchmark antioxidant, BHT. The anti-inflammatory test demonstrated that the mixture administered orally at a dose of 150 mg/kg has a better activity, exceeding that of 1% Diclofenac, with a percentage of maximum inhibition of the edema of 89.99 ± 4.08. The number of cramps in the mice treated with the mixture at a dose of 150 mg/kg is significantly lower (29.80 ± 1.92) than those of the group treated with Tramadol (42.00 ± 2.70), respectively. The toxicity results show no signs of toxicity with an LD50 greater than 150 mg/Kg. These interesting results show that the two plants’ EOs had an important anti-inflammatory, analgesic, and antioxidant activity, and also a powerful synergistic effect, which encourages further in-depth investigations on their pharmacological proprieties.
Plants are traditionally utilized in Algeria to treat many disorders, such as
Artemisia herba-alba (Asteraceae family), which is a North African species.
In Algeria, it is characterized by an ...excellent fodder fee and by a
composition of essential oils having antiseptic, vermifuge, and
antispasmodic properties. The present study aims to investigate the
antioxidant and antimicrobial activities and chemical composition of the
essential oil of Artemisia herba-alba found in northeast Algeria (Khenchela
region). Artemisia herba-alba essential oil was extracted by
hydrodistillation and its chemical composition was identified by GC/MS. In
addition, the antioxidant activity of the extracted essential oil was
determined using the DPPH assay. The agar disc diffusion method was used to
determine how well the essential oil killed germs. The essential oil was
analyzed by GC/MS, revealing 35 constituents, of which the major ones were
identified as Thujone (10.55%), borneol (5.98%), and eucalyptol (1.63%). The
observed IC50 values for the DPPH assay were 18.44 ? 0.006 mg/mL. On the
other hand, this oil proved effective against all tested strains in the
22.67 ? 3.215 mm range, including Listeria innocua CIP 74915.
The purpose of this study was to determine the chemical composition and to evaluate the genotoxic and antigentoxic activites of the Moroccan Artemisia herba-alba ethanolic extract. The ethanolic ...extract of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy (GC-MS). The mutagenic and antimutagenic activities were evaluated using thesomatic mutation and recombination test (SMART) on Drosophila melanogaster. Methyl methanesulfonate (MMS) was used as the positive control.
Twenty-one compounds of the Artemisia herba-alba ethanol extract were identified; the main monoterpenes identified are camphor, α-thujone and β-thujone. Ethanolic extract of Artemisia herba-alba did not show gentotoxicity at doses used in SMART Assays. In the co-treatment, a dose-dependent decrease in mutation frequency was observed for this plant. Ethanolic extract of Artemisia herba-alba at 1.5% demonstrated a marked decrease in MMS with a strong inhibitory effect (80%). The results demonstrate that the Artemisia herba-alba exerted a significant and potent anti-mutagenic activity in wing and eye-spot of D. melanogaster.
This study characterized the phenolic, aroma compositions, and antioxidant capacities of four Artemisia herba alba accessions collected from north and center regions in Tunisia in order to select ...valuable origin with optimal bioactive compounds production. The total polyphenol and flavonoid contents varied between accessions with maxima contents observed in center regions. Kaempherol, apigenin, naringenin, p -coumaric, trans -cinnamic, and caffeic acids were the most abundant compounds with percentage varying depending on the considered accession. Significant changes on essential oil yields (0.4–1.2%) were found between accessions and three different oil chemotypes were distinguished: camphor, fenchol/α-thujone, and α-thujone/camphor. Cluster analysis of volatile and phenolic compositions grouped the accessions on different groups. Antioxidant activities of extracts were found to be higher than essential oils for the four provenances. The results suggested that the center regions have high potential for selecting varieties rich on essential oil, antioxidant phenolic acids, and flavonoids.
Species from genus Artemisia are widely distributed throughout temperate regions of the northern hemisphere and many cultures have a long-standing traditional use of these plants as herbal remedies, ...liquors, cosmetics, spices, etc. Nowadays, the discovery of new plant-derived products to be used as food supplements or drugs has been pushed by the exploitation of bioprospection approaches. Often driven by the knowledge derived from the ethnobotanical use of plants, bioprospection explores the existing biodiversity through integration of modern omics techniques with targeted bioactivity assays. In this work we set up a bioprospection plan to investigate the phytochemical diversity and the potential bioactivity of five Artemisia species with recognized ethnobotanical tradition (A. absinthium, A. alba, A. annua, A. verlotiorum and A. vulgaris), growing wild in the natural areas of the Verona province. We characterized the specialized metabolomes of the species (including sesquiterpenoids from the artemisinin biosynthesis pathway) through an LC-MS based untargeted approach and, in order to identify potential bioactive metabolites, we correlated their composition with the in vitro antioxidant activity. We propose as potential bioactive compounds several isomers of caffeoyl and feruloyl quinic acid esters (e.g. dicaffeoylquinic acids, feruloylquinic acids and caffeoylferuloylquinic acids), which strongly characterize the most antioxidant species A. verlotiorum and A. annua. Morevoer, in this study we report for the first time the occurrence of sesquiterpenoids from the artemisinin biosynthesis pathway in the species A. alba.
Pregnant women prefer herbal medicines more than pharmaceutical drugs due to the cultural belief that herbs are more suffer during pregnancy for an accurate foetus development. Artemisia herba-alba ...(Asteraceae) is one of the most used plants in the Mediterranean region to treat various diseases including diabetes, hypertension, spasmodic dysphonia and some bacterial infection.
The present study aimed to investigate the effect of Artemisia herba-alba consumption during pregnancy on fertility, physical and behavior developments of mice offspring from birth-to-weaning days.
Female pregnant mice were divided into three groups and orally administrated with 80 and 150 mg/kg/day of the methanol extract of Artemisia h.a respectively, during the entire period of gestation. At birth, total fertility rate was counted. Body development; neuromotor reflex and behavior were also examined in mice offspring
Artemisia h.a (Aha) exposure significantly decreased the fertility ratio in both Aha-treated groups and increased the weight and length of mice offspring in 80 mg/kg/day Aha-exposed group. Moreover, Aha administration prolonged the time of completing the reflex response of surface righting, negative geotaxis, cliff avoidance and jumping test of mice offspring in Aha-exposed groups.
The present study provides strong evidence that discourage the use of Artemisia h.a during gestation period.
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