Four caffeoylquinic acid (CQA) derivatives, 5‐O‐caffeoylquinic acid (1), 3,5‐di‐O‐caffeoylquinic acid (3), 4,5‐di‐O‐caffeoylquinic acid (4), and 3,4,5‐tri‐O‐caffeoylquinic acid (5), have been ...isolated from Artemisia herba‐alba growing wild in Algeria, using the on‐line HPLCDADDPPH radical‐scavenging detection technique as guidance. In the course of the purification work, the non‐frequent (E)‐2‐(β‐D‐glucopyranosyloxy)‐4‐methoxycinnamic acid (2) has also been isolated. The CQAs showed fair‐to‐good antioxidant activities determined by the DPPH. scavenging assay. The structures of the five isolated compounds were determined by spectroscopic methods. The on‐line HPLCDADDPPH technique allowed for a rapid pinpointing of antioxidants in the studied plant, accomplishing the facile guided isolation of the target molecules. Algerian A. herba‐alba could be an interesting source of natural antioxidants that deserve further work.
The chemical variability of the essential oils of Artemisia herba-alba Asso aerial parts, collected at Algeria was evaluated. A. herba-alba populations were collected in four regions, Benifouda; ...Bougaa; Boussaada and Boutaleb, at two different periods, July (flowering phase), and October and November (vegetative phase). The essential oils were isolated by hydrodistillation and analyzed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS). The essential oils yield ranged between 0.2% and 0.9% (v/d.w.). Fifty components were identified in A. herba-alba oils, oxygen-containing monoterpenes being dominant in all cases (72–80%). Camphor (17–33%), α-thujone (7–28%) and chrysanthenone (4–19%) were the major oil components. Despite the similarity in main components, three types of oils could be defined, (a) α-thujone : camphor (23–28:17–28%), (b) camphor : chrysanthenone (33:12%) and (c) α-thujone : camphor : chrysanthenone (24:19:19%). The comparison between the present data and an updated survey of the existing literature reinforces the major variability of A. herba-alba essential oils and stresses the importance of obtaining a defined chemical type crop production avoiding the wild harvest.
Herpes simplex virus (HSV) can infect millions of people worldwide causing mild to life-threating infections. The current study demonstrates the first comparative anti-HSV type 1 activity and ...phytochemical investigation of
and
collected from Egypt and Libya. Liquid chromatography/mass spectrometry (LC/MS) analysis allowed the identification of 56 and 38 compounds in the Egyptian and Libyan
ethanolic extracts, respectively, in addition to 46 and 50 compounds in the Egyptian and Libyan
ethanolic extracts, respectively. Gas chromatography/mass spectrometry (GC/MS) analysis of their corresponding essential oils revealed the presence of 15, 17, 17 and 8 compounds in Egyptian and Libyan
and
respectively. The major chemical classes of the identified compounds were phenolic acids, flavonoids and oxygenated monoterpenes. Evaluation of the anti-HSV1 activities of the studied extracts showed that the Egyptian
ethanolic extracts were the most potent extract with more than 200-fold reduction in the viral PFU.
Varroosis induced by
Varroa destructor
Anderson and Trueman represents the most pathogenic and destructive disease affecting the western honey bee,
Apis mellifera.
In this study, we investigated the ...acaricidal activity against the
Varroa
mite using essential oils (EOs) from the aerial parts of four autochthonous Algerian herbal species, namely
Artemisia herba alba
,
Artemisia campestris
,
Artemisia judaica
and
Ruta montana
. EOs were obtained by means of hydrodistillation and their composition was characterized by gas chromatography–mass spectrometry. The toxicity of the selected EOs toward
V. destructor
and
A. mellifera
adult honey bees was evaluated using the complete exposure method. The results indicate the predominance of davanone (66.9%) in
A. herba alba
, β-pinene (19.5%) in
A. campestris
, piperitone (68.7%) in
A. judaica
and 2-undecanone (70.1%) in
R. montana
EOs. Interestingly, the LC
50
values coupled to bee mortality rates revealed that all tested oils exhibited significant acaricidal efficiency with selectivity ratio (SR) values of 10.77, 8.78, 5.62 and 3.73 for
A. campestris
,
A. judaica
,
A. herba alba
, and
R. montana
, respectively. These values were better than that of thymol (SR = 3.65), the positive control. These findings suggest that these EOs could be used as plant-derived veterinary acaricides to control varroosis in field conditions.
The essential oils were obtained by hydrodistillation from aerial parts of
L. (
.
L.) and
(
) Asso. and analyzed by gas chromatography-flame ionization detector chromatograpy (GC-FID) and gaz ...chromatography-mass spectrometry (GC-MS). The antibacterial activities of the oils were determined by the disk diffusion method and a microdilution broth assay against six bacteria stains. The combinations of these essential oils with antibiotics were evaluated against two multi-drug-resistant bacteria strains: imipenem-resistant
(IRAB S3310) and methicillin-resistant
(MRSA S19). The chemical analysis of
.
essential oil revealed the presence of pulegone (74.8%) and neoisomenthol (10.0%).
essential oil was characterized by camphor (32.0%), α-thujone (13.7%), 1,8-cineole (9.8%), β-thujone (5.0%), bornéol (3.8%), camphene (3.6%), and
-cymene (2.1%). All strains tested except
were susceptible to these oils. The combinations of essential oils with antibiotics exerted synergism, antagonism, or indifferent effects. The best effect was observed with
essential oil in association with cefoxitin (CX) against MRSA S19. However, for IRAB S3310, the strongest synergistic effect was observed with
in association with amikacin (AK). This study demonstrated that
and
essential oils have antibacterial activities which could be potentiated by antibiotics especially in the case of IRAB S3310.
Artemisia herba-alba Asso (“desert wormwood” in English; “armoise blanche” in French; “shaih” in Arabic), is a medicinal and strongly aromatic plant widely used in traditional medicine by many ...cultures since ancient times. It is used to treat inflammatory disorders (colds, coughing, bronchitis, diarrhea), infectious diseases (skin diseases, scabies, syphilis) and others (diabetes, neuralgias). In Jordanian traditional medicine, this plant is used as antiseptic and against skin diseases, scabies, syphilis, fever as well as menstrual and nervous disorders.
Considering the traditional medicinal uses and the lack of scientific studies addressing the cellular and molecular players involved in these biological activities, the present study was designed to unveil the antifungal and anti-inflammatory activities of A. herba-alba Asso essential oil at doses devoid of toxicity to mammalian cells.
Chemical analysis of A. herba-alba essential oil isolated by hydrodistillation from aerial parts was carried out by gas chromatography (GC) and gas chromatography–mass spectrometry (GC–MS). The antifungal activity (minimal inhibitory concentrations and minimal lethal concentrations) was evaluated against yeasts, dermatophyte and Aspergillus strains. In order to explore the mechanisms behind the anti-fungal effect of the essential oil, the germ tube inhibition assay was evaluated using Candida albicans. The assessment of cell viability was accomplished using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the in vitro anti-inflammatory potential of A. herba-alba oil at the periphery and central nervous system was evaluated by measuring nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated mouse macrophages and microglia, respectively.
Oxygen-containing monoterpenes are the main compounds of the oil, namely 1,8-cineole (20.1%), β-thujone (25.1%), α-thujone (22.9%) and camphor (10.5%). Among the fungal strains tested, the oil demonstrated potential against Trichophyton rubrum and Epidermophyton floccosum, with minimal inhibitory concentration (MIC) and minimal lethal concentration (MCL) values of 0.32mg/mL and Cryptococcus neoformans with MIC of 0.64mg/mL. The oil revealed a strong inhibitory effect on germ tube formation in C. albicans with inhibition of filamentation around 90% at a concentration 0.16mg/mL. Importantly, the essential oil significantly inhibited NO production evoked by LPS without cytotoxicity at concentrations up to 1.25µL/mL in macrophages and up to 0.32µL/mL in microglia. Furthermore, evaluation of cell viability in RAW 264.7 macrophages, BW2 microgliacells and HaCaT keratinocytes showed no cytotoxicity at concentrations up to 0.32μL/mL.
It was possible to find appropriate doses of A. herba-alba oil with both antifungal and anti-inflammatory activities and without detrimental effects towards several mammalian cell types. These findings add significant information to the pharmacological activity of A. herba-alba essential oil, specifically to its antifungal and anti-inflammatory therapeutic value, thus justifying and reinforcing the use of this plant in traditional medicine.
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The essential oils (EOs) of Coriandrum sativum, Carum carvi and Artemisia herba-alba have been screened for their insecticidal activity against Tribolium castaneum, Sitophilus oryzae and Lasioderma ...serricorne and for their antimicrobial activity against Gram (+), Gram (-) bacteria and against yeast strain. Artemisia herba-alba EO showed good insecticidal activity with 24-h against L. serricorne (LC
50
= 29.7) and against T. castaneum (66.1 µg/mL) and antibacterial potentials against Staphyloccocus aureus (MIC = 0.125 mg/mL). The antimicrobial activities of C. carvi EO, rich in D-carvone (72.4%) and D-limonene (23.8%) were particularly remarkable, with LC
50
of 27.9 µg/mL against L. serricorne. Similarly, coriander EO, with linalool (64.6%) as the major compound was selected for its antimicrobial activities (Candida albicans, MIC = 1 mg/mL). These findings demonstrated that the tested EOs could be used as a natural source of insecticidal and antimicrobial activity, which may have a variety of uses in the food and pharmaceutical sectors.
Background and aim: In the present study, we investigate the phytochemical composition and the nephroprotective effects as well as the antioxidant properties of Artemisia herba alba aqueous extract ...in alloxan-induced experimental diabetes in rats. Experimental procedure: Wistar rats were divided into four groups of seven rats each: Group I: Normal control (NC) received saline solution at (9 per mille) given by intraperitoneal way; Group II: Diabetic control (DC) received alloxan (150 mg/kg b.w) intraperitoneally; Group III: Normal control (NC+AHA) received saline solution at (9 per mille) and treated orally by AHA aqueous extract (400 mg/kg/b.w); Group IV: Diabetic control (DC+AHA) received alloxan solution (150 mg/kg b.w) intraperitoneally and treated by aqueous extract of AHA (400 mg/kg/b.w/day) orally after one week of alloxan administration. After 30 days, blood and tissue samples were collected for biochemical and histopathological analysis, respectively. Glomerular damage markers, including creatinine, serum urea, urine creatinine and urine urea levels were estimated. Creatinine clearance was also assessed. Oxidative stress parameters were assessed in the kidney homogenate. Results and conclusion: Alloxan-exposure resulted in significant increase in blood glucose and serum level of glomerular damage markers. The antioxidant enzyme activities were significantly downregulated associated with an increase in malondialdehyde (MDA) level over the baseline values. Artemisia herba alba aqueous extract supplementation significantly improved the studied parameters. In concluding, the results obtained suggests that Artemisia herbs-alba aqueous extract supplementation reduces alloxan-induced free radical generation, potentiates the antioxidant defense system and alleviates renal sensitivity to oxidative stress.
Arthritis is a debilitating condition impacting the quality of life for millions worldwide, characterized by pain and inflammation. Understanding the mechanisms of arthritis and developing effective ...treatments are crucial. This study investigated the hydroethanolic extract of
Artemisia herba-alba
for its protective potential against arthritis hallmarks, oxidative stress, and lipid peroxidation in vitro. It also assessed its in vivo anti-arthritic activity. The phytochemical analysis identified various compounds within the extract, with high concentrations of polyphenols and flavonoids. These compounds are associated with numerous health benefits, making
A. herba-alba
a potential source of valuable phytochemicals.
A. herba-alba
demonstrated a notable effect in body weight loss, paw edema, and arthritic severity. Histopathological examination revealed structural improvements in bone and muscle tissues, emphasizing its therapeutic potential in managing chronic arthritis. Furthermore, while these findings are promising, further studies are necessary to delve deeper into the mechanisms underlying the observed hematological changes and to gain a more comprehensive understanding of the in vivo results. This research sets the stage for continued exploration, ultimately aiming to unlock the full potential of
A. herba-alba
in addressing chronic arthritis and enhancing the lives of those affected by this condition.
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