•A combination of several steps makes the method more sensitive and selective.•Validation with the accuracy profile was done for glyphosate and glufosinate.•Method was applied to the analysis of 136 ...food samples and to routine analysis.
A reliable and sensitive method was developed for simultaneous determination of glyphosate and glufosinate in various food products by liquid chromatography-tandem mass spectrometry. Based on extraction, derivatization with 9-fluorenylmethylchloroformate and purification on solid phase extraction column, quantification was done by using isotopic-labeled analytes as internal standard and calibration in matrix. Good selectivity and sensitivity were achieved with a limit of quantification of 5 μg/kg. The recoveries of these two pesticides ranged from 91% to 114% with inter-day and relative standard deviation of 3.8–6.1% in five matrices of cereal group spiked at 5, 10, and 20 μg/kg. An accuracy profile was performed for method validation, demonstrating the accuracy and precision of the method for the studied food groups. The verification results in expanded food groups indicated extensive applicability for the analysis of glyphosate and glufosinate. Finally, the developed method was applied to analyze 136 food samples including milk-based baby foods from the French Agency for Food, Environmental and Occupational Health & Safety. Glyphosate residues were detected in two breakfast cereal samples (6.0 and 34 μg/kg). Glufosinate residues were found in a sample of boiled potatoes (9.8 μg/kg). No residues were detected in the other samples, including milk-based baby foods with limits of detection ranging from 1 to 2 μg/kg. The method has been applied for routine national monitoring of glyphosate and glufosinate in various foods.
Azo-dyes such as Allura Red, Carmoisine, Amaranth, Sunset Yellow (SY), Brilliant Blue, Tartrazine (Tz), etc., are popular as food coloring agents due to their low cost and stability. SY and Tz are ...the most used members of this group of dyes since they have similar colors and are usually used together in food products. Despite their advantageous industrial use, they exhibit a risk toxicity profile with adverse effects such as allergy, asthma, carcinogenicity, genotoxicity, cytotoxicity, anxiety, etc. Therefore, the United States Food and Drug Administration (FDA) and European Food Safety Authority (EFSA) regulate the permissions for using these compounds to provide safe food products for consumers and prevent adverse effects both short and long-term. Considering all of these, for the analysis of azo toxic dyes, highly sensitive, low-cost, simple, and rapid sensors are necessary. Electrochemical nanosensors, which combine the unique features of electrochemistry and nanotechnology, are devices with all these advantages and are widely used for the determination of azo dyes. SY and Tz step forth as the most used food dyes in the class of azo-toxic dyes. They are often preferred together in food products, increasing the occurrence and exposure risk. Therefore, the analysis of Sunset Yellow and Tartrazine in food products has significant importance.
In this review, the latest nanomaterial-based approaches for the electrochemical sensors on the analysis of SY and Tz in food samples were evaluated in terms of used nanomaterials and applied food samples.
Display omitted
Fingerprinting methods describe a variety of analytical methods that provide analytical signals related to the composition of foodstuffs in a non-selective way such as by collecting a spectrum or a ...chromatogram. Mathematical processing of the information in such fingerprints may allow the characterisation and/or authentication of foodstuffs. In this context, the particular meaning of 'fingerprinting', in conjunction with 'profiling', is different from the original meanings used in metabolomics. This fact has produced some confusion with the use of these terms in analytical papers. Researchers coming from the metabolomic field could use 'profiling' or 'fingerprinting' on a different way to researchers who are devoted to food science. The arrival of an eclectic discipline, named 'foodomics' has not been enough to allay this terminological problem, since the authors keep on using the terms with both meanings. Thus, a first goal of this tutorial is to clarify the difference between both terms. In addition, the chemical approaches for food authentication, i.e., chemical markers, component profiling and instrumental fingerprinting, have been described. A new term, designated as 'food identitation', has been introduced in order to complete the life cycle of the chemical-based food authentication process. Chromatographic fingerprinting has been explained in detail and some strategies which could be applied has been clarified and discussed. Particularly, the strategies for chromatographic signals acquisition and chromatographic data handling are unified in a single framework. Finally, an overview about the applications of chromatographic (GC and LC) fingerprints in food authentication using different chemometric techniques has been included.
Display omitted
•Chemical approaches for food authentication, i.e., chemical markers, component profiling and instrumental fingerprinting, have been described.•Difference between chromatographic signals and data are clarified.•The food authentication framework using chromatographic fingerprinting has been properly established and discussed.•A new term, named food ‘identitation’, has been proposed as a prior and necessary stage in order to close the life cycle of the food authentication process.•Right analytical applications of chromatographic fingerprinting on food ‘identitation’ and food authentication have been reviewed.
•Up-to-date summary of the use of electronic noses in analysis of edible oils.•E-nose applications in determination of botanical and geographical origin of oils.•E-nose applications in detection of ...adulterations and quality control.•Comparison of electronic olfaction with other methods of oil quality evaluation.
The growing demand for wholesome and nutritious food leads to intensification of production, which in turn can have a detrimental effect on quality and well-being of consumers. For that reason, it is important to develop novel methods of food control which would be characterized by a short time of analysis, adequate sensitivity and relatively low cost. One such technique involves the use of multi-sensory devices called electronic noses. In recent years there has been a rapid development of this method, especially in the area of food control. Electronic olfaction can be successfully used in the analysis of edible oils, in particular in the determination of the product’s geographical origin, and in detection of adulteration as well as deterioration caused by external factors.
Fragmentation characteristics are crucial for nontargeted screening to discover and identify unknown exogenous chemical residues in animal-derived foods. In this study, first, fragmentation ...characteristics of 51 classes of exogenous chemical residues were summarized based on experimental mass spectra of standards in reversed-phase and hydrophilic interaction liquid chromatography–high-resolution mass spectrometry (MS) and mass spectra from the MassBank of North America (MoNA) library. According to the proportion of fragmentation characteristics to the total number of chemical residues in each class, four screening levels were defined to classify 51 classes of chemical residues. Then, a nontargeted screening method was developed based on the fragmentation characteristics. The evaluation results of 82 standards indicated that more than 90 % of the chemical residues with MS/MS spectra can be identified at concentrations of 100 and 500 μg/kg, and about 80 % can be identified at 10 μg/kg. Finally, the nontargeted screening method was applied to 16 meat samples and 21 egg samples as examples. As a result, eight chemical residues and transformation products (TPs) of 5 classes in the exemplary samples were found and identified, in which 3 TPs of azithromycin were identified by fragmentation characteristics-assisted structure interpretation. The results demonstrated the practicability of the nontargeted screening method for routine risk screening of food safety.
Display omitted
•Fragmentation characteristics of 51 classes of chemical residues were summarized.•A nontargeted screening method was proposed to discover unknown chemical residues.•The method had a great screening performance in the evaluation by 82 standards.•Eight chemical residues and TPs of 5 classes were identified in egg and meat.
•Deep eutectic solvents (DESs) are versatile extractants of pesticides in food.•DES-based DLLME is a green pre-concentration technique for pesticides in food.•Modifications make DES-based DLLME ...greener during pesticide analysis.•Hyphenation of DES-based DLLME technique improves its extraction efficiency.
Deep eutectic solvents are versatile, green and new generation solvents that can be used during dispersive liquid-liquid micro-extraction techniques for pesticides. They have tunable physico-chemical properties that can be easily changed by varying the ratios of hydrogen bond donors and hydrogen bond acceptors in their structures. Deep eutectic solvents are non-flammable, chemically and thermally stable solvents with low vapour pressure. Thus, they have characteristics that are similar to those of ionic liquids. However, they have simpler synthetic procedures, less expensive and are more biodegradable than ionic liquids. One of the limitations of deep eutectic solvents is their toxicity to the environment but they are less toxic than ionic liquids. This paper gives a focused and comprehensive recent review on the applications of deep eutectic solvents during dispersive liquid-liquid micro-extraction of pesticides in food samples for the period starting from 2016 to 2020. Emphasis was placed on the modifications done to the deep eutectic solvent-based dispersive liquid-liquid micro-extraction techniques in order to enhance their greenness during pesticide pre-concentration in food samples. In addition, hyphenated dispersive liquid-liquid micro-extraction techniques were also reviewed and lastly, the paper outlined the challenges associated with the use of DESs during the DLLME techniques.
Food analysis plays a critical role in assessing human health risks and monitoring food quality and safety. Currently, there is a pressing need for a reliable, portable, and quick recognition element ...for point-of-care testing (POCT) to better serve the demands of on-site food analysis. Aptamer-modified paper-based analytical devices (Apt-PADs) have excellent characteristics of high portability, high sensitivity, high specificity, and on-site detection, which have been widely used and concerned in the field of food safety. The article reviews the basic components and working principles of Apt-PADs, and introduces their representative applications detecting food hazards. Finally, the advantages, challenges, and future directions of Apt-PADs-based sensing performance are discussed, to provide new directions and insights for researchers to select appropriate Apt-PADs according to specific applications.
•The special composition and structure of Apt-PADs are systematically described.•In-depth description of the sensing applications of Apt-PADs and the research progress in recent years.•Intervention strategies of aptamers and paper bases to obtain high sensitivity of Apt-PADs.•Specific outlook on the future direction of Apt-PADs improvements.
Current techniques for the detection of multi-pesticides are limited by technical complexity, sensitivity and cost. There is an urgent demand of developing new specific recognition elements and ...sensitive signal readouts for on-site monitoring. In this work, we developed a fluorescent aptamer-based lateral flow biosensor (apta-LFB) integrated with fluorophore-quencher nano-pairs and a smartphone spectrum reader to accomplish triple-target detection of chlorpyrifos, diazinon, and malathion. Aptamers serve as alternative recognition elements instead of antibodies in LFB, offering better specificity and stability. A novel fluorophore-quencher nano-pair (quantum dots nanobeads and gold nanostars) was implemented to perform “signal-on” instead of “signal-off”. After optimization, detection limit of chlorpyrifos, diazinon, and malathion were determined to be 0.73 ng/mL, 6.7 ng/mL, and 0.74 ng/mL, respectively. Greatly increased sensitivity may come from the combination of improved signal and zero background. This innovative cascade strategy allowed to lower the detection limit of pesticides residue level in food, which is largely considered satisfactory. The accuracy and practicality of this design for effective on-site quantification of multi-pesticides were further confirmed using 12 vegetable and fruit samples. The estimated recoveries were between 82.4% and 112.8% in spiked vegetable samples, which indicated that the developed method is capable for detecting multi-pesticides in food samples. This sensitive handheld-system is promising to become a powerful tool for practical on-site application of multi-pesticide quantification procedures.
•We developed a fluorescent apta-LFB integrated with fluorophore-quencher nano-pairs and a smartphone spectrum reader.•This cascade strategy accomplished sensitive and triple-target detection of chlorpyrifos, diazinon, and malathion.•A novel fluorophore-quencher nano-pair was implemented to perform “signal-on” instead of “signal-off”.•Detection limit of chlorpyrifos, diazinon, and malathion were 0.73 ng/mL, 6.7 ng/mL, and 0.74 ng/mL, respectively.
The lack of specific, low-cost, rapid, sensitive, and easy detection of biomolecules has resulted in the development of biosensor technology. Innovations in biosensor technology have enabled many ...biosensors to be commercialized and have enabled biomolecules to be detected onsite. Moreover, the emerging technologies of lab-on-a-chip microdevices and nanosensors offer opportunities for the development of new biosensors with much better performance. Biosensors were first introduced into the laboratory by Clark and Lyons. They developed the first glucose biosensor for laboratory conditions. Then in 1973, a glucose biosensor was commercialized by Yellow Springs Instruments. The commercial biosensors have small size and simple construction and they are ideal for point-of-care biosensing. In addition to glucose, a wide variety of metabolites such as lactate, cholesterol, and creatinine can be detected by using commercial biosensors. Like the glucose biosensors (tests) other commercial tests such as for pregnancy (hCG), Escherichia coli O157, influenza A and B viruses, Helicobacter pylori, human immunodeficiency virus, tuberculosis, and malaria have achieved success. Apart from their use in clinical analysis, commercial tests are also used in environmental (such as biochemical oxygen demand, nitrate, pesticide), food (such as glutamate, glutamine, sucrose, lactose, alcohol, ascorbic acid), and biothreat/biowarfare (Bacillus anthracis, Salmonella, Botulinum toxin) analysis. In this review, commercial biosensors in clinical, environmental, food, and biowarfare analysis are summarized and the commercial biosensors are compared in terms of their important characteristics. This is the first review in which all the commercially available tests are compiled together.
PDF
