The loop‐mediated isothermal amplification (LAMP) method is one of the Nucleic acid amplification tests (NAATs) that allows for the amplification of target regions without using a thermal cycle. With ...its unique primer design, LAMP ensures the rapid replication of the targeted DNA region with high specificity and high efficiency. LAMP technology is used for diagnostic purposes in pathogen detection due to its ease of use, low cost, and simplicity without requiring complex equipment. A wide range of LAMP diagnostic platforms have been developed for applications in bacteria, virus, and parasitic pathogen detection. Herein, the methodology of LAMP technology and its applications in pathogen detection and SNP genotyping and mutation detection are discussed. Point‐of‐care (PoC) LAMP platforms designed with the principles of microfluidic chip technology, including LAMP‐on‐a‐chip, paper‐based LAMP, and smartphone‐based LAMP applications have been elaborated. LAMP technology represents a fast, robust, and reliable diagnostic platform for point‐of‐care testing.
Loop‐mediated isothermal amplification (LAMP) technology is used for diagnostic purposes in pathogen detection because it is fast, cost‐effective, and straightforward. It has applications in bacteria, viruses, and parasitic pathogen detection. LAMP technology has gained more popularity recently by conversion to pathogen‐specific point‐of‐care (PoC)‐LAMP diagnostic platforms. These new‐generation platforms include LAMP‐on‐a‐chip, paper‐based LAMP, and smartphone‐based LAMP applications.
Nucleic acid amplification for the detection of infectious diseases, food pathogens, or assessment of genetic disorders require a laboratory setting with specialized equipment and technical ...expertise. Isothermal deoxyribonucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP), exhibit characteristics ideal for point-of-care (POC) applications, since their instrumentation is simpler in comparison with the standard method of polymerase chain reaction. Other key advantages of LAMP are robustness and the production of pyrophosphate in the presence of the target gene, enabling to detect the reaction products using the naked eye. Polymerase inhibitors, presented in clinical samples, do not affect the amplification process, making LAMP suitable for a simple sample-to-answer diagnostic systems with simplified sample preparation. In this review, we discuss the trends in miniaturized LAMP techniques, such as microfluidic, paper-based, and digital with their advantages and disadvantages, especially for POC applications alongside our opinion of the future development of miniaturized LAMP.
•Introduction of loop mediated isothermal amplification (LAMP) and its principle.•Classical microfluidics-based LAMP for DNA/RNA detection.•Paper-based LAMP.•Microfluidic-based digital LAMP.•Future of microfluidic LAMP development.
The loop-mediated isothermal amplification (LAMP) technique is a great alternative to PCR-based methods, as it is fast, easy to use and works with high sensitivity and specificity without the need ...for expensive instruments. However, one of the limitations of LAMP is difficulty in achieving the simultaneous detection of several targets in a single tube, as the methodologies that allow this rely on fluorogenic probes containing specific target sequences, complicating their adaptation and the optimization of assays. Here, we summarize different methods for the development of multiplex LAMP assays based on sequence-specific detection, illustrated with a schematic representation of the technique, and evaluate their practical application based on the real-time detection and quantification of results, the possibility to visualize the results at a glance, the prior stabilization of reaction components, promoting the point-of-care use, the maximum number of specific targets amplified, and the validation of the technique in clinical samples. The various LAMP multiplexing methodologies differ in their operating conditions and mechanism. Each methodology has its advantages and disadvantages, and the choice among them will depend on specific application interests.
•Four immunosuppressive viruses in chicken can be detected with LAMP.•The results of detection can be observed visually by a change in color.•The detection methods show high specificity and ...sensitivity.
Loop-mediated isothermal amplification (LAMP) methods to detect chicken infectious anemia virus (CIAV), reticuloendotheliosis virus (REV), and Marek’s disease virus (MDV), and a reverse transcription (RT)-LAMP assay to detect infectious bursal disease virus (IBDV), were developed. The CIAV-LAMP, REV-LAMP, MDV-LAMP, and IBDV-RT-LAMP methods were performed using four sets of six primers targeting the VP1 gene of CIAV, the gp90 gene of REV, the Meq gene of MDV, and the VP2 gene of IBDV. The results (a change in color) were observed visually. The methods showed high specificity and sensitivity. The detection limits were 50 genomic copies of CIAV, 16 genomic copies of REV, 20 genomic copies of MDV, and 250 genomic copies of IBDV. When used to test clinical samples, the results of the LAMP assays were in 100% agreement with a previously described PCR. Therefore, the LAMP assays are simple, rapid, highly sensitive, and specific methods for detecting four immune-suppressive viruses.
Introduction. In modern society, different types of lighting fixtures with a variety of shapes, colours and brightness are widely used. However, not all of them effectively fulfil their functions and ...can negatively affect human health. Optimal lighting parameters are identified, taking into account safety and energy efficiency. Key aspects include the analysis of the spectra of incandescent, fluorescent and LED lamps using a spectrometer and assessing their impact on the vision and mental health of employees working in an office space through a written survey.Materials and methods. The illuminance, luminous flux and economy of workstations in office premises were analyzed.Results. The analysis of the obtained results allowed to determine the rationality of application of the investigated spectra of different types of lamps. The results of the research will be useful both for specialists and manufacturers in the field of lighting engineering, as well as end users.Conclusions. Recommendations for the use of LED lamps as the main source of lighting in office-type premises are given. These lamps provide an almost uniform spectrum, which creates optimal conditions for vision and reduces the negative impact on mental health. It is proposed to supplement the workspace lighting with individual light sources in the form of desk lamps of a yellowish shade at each employee's workplace. LED lamps are not only energy efficient, but also contribute to the formation of a comfortable and safe working environment.
Proper management and treatment of end-of-life fluorescent lamps with a toxic metal of mercury has attracted critical concern in the solid waste community. In this study, material flow analysis (MFA) ...and substance flow of mercury were performed on the lamps by life cycle in South Korea. It was found that, in 2020, approximately 2957 tons (or 27.9 million units) of end-of-life fluorescent lamps from households were collected and recycled by the recycling facilities in South Korea. Approximately 278 kg of mercury was recovered from the lamps and treated at the hazardous incineration facilities. Based on the results of dynamic flow analysis, the amount of fluorescent lamps to be retried is expected to continually decrease to be about 14.2 million units, which is estimated to be 23 kg of mercury. However, continued collection efforts on end-of-life fluorescent lamps owing to increasing demands for light-emitting diode lamps should be made from the perspectives of proper treatment of mercury as well as resource recovery. More detailed studies on other mercury-containing lamps (e.g., metal halogen lamps, high-pressure mercury lamps, and high-pressure sodium lamps) are warranted to determine mercury flows in waste streams for proper collection and treatment upon disposal.
A novel, simple, and inexpensive model for teaching depth of lesions within the cornea using slit lamp biomicroscopy to veterinary students, ophthalmology residents, and general clinicians with ...access to a slit lamp biomicroscope is described. Using common laboratory items, this method can be created in any clinic and can be used to teach and quiz students through independent study with objective self‐testing achieved.
In this paper, an integrated double buck-boost (IDBB) converter is proposed as a high-power-factor offline power supply for power-LED lamps. The IDBB converter features just one controlled switch and ...two inductors and is able to supply a solid-state lamp from the mains, providing high power factor and good efficiency. In this paper, the IDBB converter is analyzed, and a design methodology is proposed. It is demonstrated that, with a careful design of the converter, the filter capacitances can be made small enough so that film capacitors may be used. In this way, the converter mean time between failures can be made as high as that of the solid-state lamp. A design example for a 70-W converter supplied from a 230 V/50 Hz mains for street lighting applications is shown. Finally, experimental results from a laboratory prototype are also presented.
•Isothermal assay protocol was developed to detect RNA plant virus.•Improved sensitivity was achieved.•Test allows point of care diagnostic of wheat infecting pathogen.•Cost effectiveness was ...achieved by reducing reagents such as enzyme concentration.
Wheat stripe mosaic virus (WhSMV) is a significant wheat pathogen that causes substantial yield losses in Brazil and other countries. Although several detection methods are available, reliable and efficient tools for on-site WhSMV detection are currently lacking. In this study, a Loop-Mediated Isothermal Amplification (LAMP) method was developed for rapid and reliable field detection of WhSMV. We designed WhSMV-specific primers for the LAMP assay and optimized reaction conditions for increased sensitivity and specificity using infected plant samples.
We have developed a diagnostic method utilizing the Loop-Mediated Isothermal Amplification (LAMP) technique capable of rapidly and reliably detecting WhSMV. The LAMP assay has been optimized to enhance sensitivity, specificity, and cost-effectiveness.
The LAMP assay described here represents a valuable tool for early WhSMV detection, serving to mitigate the adverse economic and social impacts of this viral pathogen. By enabling swift and accurate identification, this assay can significantly improve the sustainability of cereal production systems, safeguarding crop yields against the detrimental effects of WhSMV.
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