A novel series of imidazothiadiazole‐linked benzenesulfonamide derivatives (5a–t) was synthesized and subjected for screening against the four physiologically and pharmacologically relevant human ...carbonic anhydrase (hCA) isoforms: hCA I, II, VA, and IX. The compounds selectively inhibited hCA I and II over hCA VA and IX. Furthermore, among the two cytosolic isoforms, hCA II was more effectively inhibited as compared with hCA I. The most active compounds were 5o with Ki = 0.246 µM and 5p with Ki = 0.376 µM against hCA II, whereas compound 5f showed good inhibition against both hCA I and II with Ki = 0.493 and 0.4 µM, respectively. This class of underexplored sulfonamides may be used to design isoform‐selective CA inhibitors targeting enzymes of medicinal chemistry interest.
The pincer ligand 2,4-bis(3,5-dimethyl-1H-pyrazol-1-yl)-6-methoxy-1,3,5-triazine (bpmt) was used to synthesize the novel Na(bpmt)sub.2AuClsub.4 complex through the self-assembly method. In this ...complex, the Na(I) ion is hexa-coordinated with two tridentate N-pincer ligands (bpmt). The two bpmt ligand units are meridionally coordinated to Na(I) via one short Na-Nsub.(s-triazine) and two slightly longer Na-Nsub.(pyrazole) bonds, resulting in a distorted octahedral geometry around the Na(I) ion. In the coordinated bpmt ligand, the s-triazine core is not found to be coplanar with the two pyrazole moieties. Additionally, the two bpmt units are strongly twisted from one another by 64.94°. Based on Hirshfeld investigations, the H···H (53.4%) interactions have a significant role in controlling the supramolecular arrangement of the Na(bpmt)sub.2AuClsub.4 complex. In addition, the Cl···H (12.2%), C···H (11.5%), N···H (9.3%), and O···H (4.9%) interactions are significant. Antimicrobial investigations revealed that the Na(bpmt)sub.2AuClsub.4 complex has promising antibacterial and antifungal activities. The Na(bpmt)sub.2AuClsub.4 complex showed enhanced antibacterial activity for the majority of the studied gram-positive and gram-negative bacteria compared to the free bpmt (MIC = 62.5-125 µg/mL vs. MIC = 62.5-500 µg/mL, respectively) and Amoxicillin (MIC > 500 µg/mL) as a positive control. Additionally, the Na(bpmt)sub.2AuClsub.4 complex had better antifungal efficacy (MIC = 125 µg/mL) against C. albicans compared to bpmt (MIC = 500 µg/mL).
The current study deal with 214 clinical samples taken from inand outpatient's of both sexes with different ages of Al-Hilla Teaching Hospital, Babylon Maternity and Pediatrics Hospital in Hilla ...city. All tests were conducted in the laboratories of the University of Kufa, College of Science, Department of Ecological and Pollution Sciences, in 2022.57 bacterial isolates (26.6%) were isolated and identified according to their phenotypic characteristics and biochemical tests. The current study declared that the isolation percentage was 39 (18.2%) and 18 (8.4%) for S. aureus and S. epidermidis respectively. The antibiotics sensitivity to isolates were tested, as it included 27 antibiotics, by disk diffusion method, most of it showed high resistance. Meropenem, ceftriaxone, and amikacin were found to be the most effective antibiotics, the phytosynthesized ZnO (NPs) were also tested on the sensitivity of the isolates. The lowest inhibitory concentrations (MIC) for current isolates were high. While the ZnO (NPs) showed higher efficiency and lower concentrations. This study is important in pharmacological and epidemiological studies and could open up broader horizons to find alternatives to the antibiotics used previously.
Objetivo: analisar, na prática clínica dos hospitais de grande porte como tem ocorrido a adoção das medidas de prevenção e controle da disseminação da resistência bacteriana e propor um escore de ...adesão das instituições. Método: estudo transversal realizado em 30 hospitais de grande porte de Minas Gerais, no período de fevereiro de 2018 a abril de 2019, após aprovação pelo Comitê de Ética e Pesquisa. Realizaram-se entrevistas com os gestores dos hospitais, com os coordenadores dos Serviços de Controle de Infecção Hospitalar e com os coordenadores assistenciais das Unidades de Internação e Centro de Terapia Intensiva. Além disso, conduziram-se observações da adoção das medidas de prevenção pela equipe multiprofissional nas unidades assistenciais. Resultados: nos 30 hospitais participantes, 93,3% (N=28) apresentaram protocolos para antibióticos profiláticos, e 86,7% (N=26) realizavam sua auditoria, 86,7% (N=26) para antibióticos terapêuticos e 83,3% (N=25) sua auditoria; 93,3% (N=56) utilizavam luvas e capotes para pacientes em precaução de contato, e 78,3% (N=47) dos profissionais desconheciam ou responderam de forma incompleta sobre os cinco momentos para higienização das mãos. No escore para identificar a adoção das medidas de controle da resistência bacteriana, 83,3% (N=25) dos hospitais foram classificados como com adesão parcial, 13,3% (N=04) com adesão deficiente e 3,4% (N=01) como não adoção. Conclusão: constatou-se que as medidas recomendadas para contenção da resistência bacteriana não estão consolidadas na prática clínica dos hospitais.
Objetivo: analizar, en la práctica clínica de los hospitales de gran porte, cómo se ha producido la adopción de medidas preventivas y de control de la propagación de resistencias bacterianas y ...proponer un puntaje de adhesión de las instituciones. Método: estudio transversal realizado en 30 hospitales de gran porte de Minas Gerais, de febrero de 2018 a abril de 2019, previa aprobación del Comité de Ética e Investigación. Se realizaron entrevistas con los administradores de los hospitales, con los coordinadores de los Servicios de Control de Infección Hospitalaria y con los coordinadores de atención de las Unidades de Internación y del Centro de Cuidados Intensivos. Además, se hicieron observaciones sobre la adopción de medidas preventivas por parte del equipo multidisciplinario en las unidades de atención. Resultados: en los 30 hospitales participantes, el 93,3% (N=28) presentaban protocolos de antibióticos profilácticos, y el 86,7% (N=26) realizaron su auditoría, el 86,7% (N=26) de antibióticos terapéuticos y el 83,3% (N=25) realizaron su auditoría; El 93,3% (N=56) utilizó guantes y batas para los pacientes en precaución de contacto, y el 78,3% (N=47) de los profesionales desconocía o respondía de forma incompleta sobre los cinco momentos de higiene de manos. En el puntaje para identificar la adopción de medidas para el control de la resistencia bacteriana, el 83,3% (N=25) de los hospitales se clasificaron como con adhesión parcial, el 13,3% (N=04) con adhesión deficiente y el 3,4% (N=01) como sin adopción. Conclusión: se descubrió que las medidas recomendadas para contener la resistencia bacteriana no están consolidadas en la práctica clínica de los hospitales.
ZusammenfassungGesteigertes Bewusstsein für die Problematik zunehmender Antibiotikaresistenzen und drohender Verlust geeigneter Therapieoptionen für Infektionen haben die Rahmenbedingungen in der ...Patientenversorgung verändert. In internationalen (WHO, ECDC) und nationalen Programmen und evidenzbasierten Leitlinien wurden Maßnahmen und Instrumente von Antibiotic Stewardship (ABS) konkretisiert. In Deutschland ist ABS im Krankenhaus im Infektionsschutzgesetz §23 verankert und Empfehlungen dazu in einer S3-Leitlinie. Das vorliegende Positionspapier stellt Ziele von ABS sowie erforderliche strukturelle und personelle Voraussetzungen für Krankenhäuser dar.Für die erfolgreiche Arbeit des ABS-Teams sind die Sicherung einer qualifizierten Fort- und Weiterbildung, die Mandatierung, die Unterstützung und die Bereitstellung ausreichender Ressourcen durch die Krankenhausleitung unabdingbar. Das ABS-Team soll krankenhausweit und abteilungsübergreifend arbeiten. Aufgaben sind Ausarbeitung und Umsetzung eines auf die lokalen Bedürfnisse und Möglichkeiten des Krankenhauses zugeschnittenen ABS-Programms unter Berücksichtigung von Antiinfektivaeinsatz, Resistenzlage und Patientenschwerpunkten. Dazu kommen Erstellung und Implementierung hausinterner Empfehlungen zur Diagnostik, Prophylaxe und Therapie wichtiger Infektionen. Das ABS-Team soll interdisziplinär arbeiten und als Teammitglieder speziell qualifizierte Ärzte und Apotheker umfassen. ABS-beauftragte Ärzte unterstützen das ABS-Team und berücksichtigen abteilungsspezifische Belange. Es wird von einer Mindestpersonalstärke für das ABS-Team von 1 Vollzeitäquivalent (VZÄ) pro 500 Betten ausgegangen. Je nach Krankenhausschwerpunkten und Anforderungen wird ein Zusatzbedarf gesehen. Die Wochenarbeitszeitstunden für einen ABS-beauftragten Arzt einer mittelgroßen Abteilung wurden auf 0,1 VZÄ pro 100 Betten geschätzt.
PDF
