Provider: - Institution: - Data provided by Europeana Collections- Prethodna istraživanja pokazala su da BPC 157 sprječava nastanak okluzivnog tromba i ubrzava razgradnju već stvorenog ugruška nakon ...formiranja anastomoze aorte, a da, s druge strane, smanjuje vrijeme krvarenja kod štakora tretiranih varfarinom, heparinom i aspirinom. Temeljem toga, nametnula se potreba određivanja njegova utjecaja na agregaciju trombocita i viskoelastična svojstva krvnog ugruška. Za ispitivanje eventualne uključenosti NO sustava u djelovanje BPC 157 korišten je selektivni inhibitor topljive gvanilil ciklaze, ODQ. Životinje (n=60) su najprije podijeljene u skupine ovisno o tome koji antiagregacijski lijek primaju (1.aspirin, 2.klopidogrel, 3.cilostazol), a te skupine su tada nasumično podijeljene u četiri podskupine koje su primale a) fiziološku otopinu, b) BPC 157, c) ODQ, d) BPC 157 + ODQ. Mjerenja su izvršena impendancijskom agregometrijom s 4 različita agonista (ADP, AA, AA/PGE1, kolagen), te rotacijskom tromboelastometrijom s 3 različita agonista (preko vanjskog i unutarnjeg puta zgrušavanja, te bez doprinosa trombocita). Iz rezultata dobivenih usporedbom podskupina a i b, te b i d unutar svake skupine možemo zaključiti da BPC 157 oporavlja inhibiranu agregaciju trombocita, ali da nema utjecaja na viskoelastična svojstva krvnog ugruška u ispitivanih štakora.- As a natural extension of previous research that confirmed the role of BPC 157 in the prevention of obstructive thrombus formation and rapid destruction of an already formed one after aortic anastomosis, but also shortening of the bleeding time in rats treated with anticoagulants and aspirin, there was a necessity to determine how BPC 157 influences platelet aggregation and viscoelastic properties of the blood clot. To assess its relation to NO system, sCG selective inhibitor (ODQ) was used. Rats (n=60) were divided into groups depending on the antiaggregatory drug they were treated with (1.aspirin, 2.clopidogrel, 3.cilostazol). Groups were further divided into four subgroups treated with a) normal saline, b) BPC 157, c) ODQ, d) BPC 157 + ODQ. Impendance aggregomery measurements with four agonists (ADP, AA, AA/PGE1 and collagen), and also rotational thromboelastometric measurements with 3 agonists (for initiating external and internal coagulation pathway and without platelet contribution) were performed. Based on the results obtained by comparing subgroups a versus b, and b versus d in each group, we can conclude that BPC 157 rescues inhibited platelet aggregation, but it has no effect on viscoelastic properties of the blood clot.- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
Provider: - Institution: - Data provided by Europeana Collections- Three small compactors with butterflies- Három tárlódobozka lepkékkel- All metadata published by Europeana are available free of ...restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
Provider: - Institution: - Data provided by Europeana Collections- The changes of colours in butterflies- A lepkék színeváltozása- All metadata published by Europeana are available free of ...restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
Provider: - Institution: - Data provided by Europeana Collections- Stopa depresivnih poremećaja u pandemijskom je porastu. Većinu samoubojstava i pokušaja samoubojstva počine depresivne osobe. Zbog ...navedenog, neophodni su pouzdani biomarkeri individualizirane procjene rizika suicidalnog ponašanja. Mjerenje koncentracije elemenata u pojedinim biološkim uzorcima omogućuje uvid u promjenu stanja dinamike metabolizma normalnih i patoloških procesa. Metabolički status bioelemenata (BE) te razina hormona i aminokiselina koje sudjeluju u regulaciji metabolizma mogu biti potencijalni biomarkeri suicidalnog ponašanja. Glavni cilj istraživanja bio je ispitati povezanost i međuodnos elektrolita i elemenata u tragovima, aminokiselina i vazopresina u plazmi ispitanika oboljelih od depresije koji su pokušali samoubojstvo (n=38) u odnosu na kontrolnu skupinu (n=47). Istraživanjem nije potvrđena povezanost koncentracije natrija sa suicidalnošću, a rizik za suicid raste sa sniženom ili prosječnom koncentracijom joda (I), litija (Li), povišenom ili prosječnom koncentracijom barija (Ba) kao i s dobi iznad 43,5 godina. Utvrđena je statistički značajno niža koncentracija asparagina kod suicidalne skupine. Potvrđena je obrnuto proporcionalna povezanost vazopresina i suicidalnosti (p<0,001). Dobiveni rezultati mogli bi biti temelj za pronalazak neurospecifičnih biomarkera za depresiju u sklopu personalizirane medicine.- Most of the suicides and suicide attempts are result of depressive disorders. Due to a pandemic increase of such disorders, most suicides and suicide attempts are committed by depressive persons, reliable biomarkers of the suicide risk are needed. Measurement of elements concentration in specific biological samples gives us an insight into metabolic dynamic change in normal and pathological processes. Metabolic status of bioelements as well as hormones and amino acids levels could be the biomarkers of suicidal behavior.The aim of research was to analyze concentracions of electrolytes and trace elements, plasma amino acids and vasopressin in depressed hospitalized suicide attempters (n=38) and to correlate the results with those in control subjects (n=47). The main hypothesis of this research was that there is a significant difference in above mentioned parameters between suicide attempters with major depression disorder and healthy control subjects. Significantly higher concentration of sodium (Na) has not been observed in suicide attempter group. Suicide risk increases with decreased or average concentration of iodine (I) and lithium (Li), increased or average concentration of barium (Ba) as well as the age > 43.5 years. We determined significantly decreased concentration of asparagine in suicide attempters. Negative correlation between arginine vasopressin and suicidal behaviour was found (p<0,001). This study could be the basis for potential development of neuro-specific biomarkers in personalized medicine.- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
Provider: - Institution: - Data provided by Europeana Collections- RSV (engl. respiratory syncytial virus) je najčešći uzročnik respiratornih infekcija djece do druge godine. Razvoj težih oblika ...bolesti i česte reinfekcije mogu se povezati s istovremenim razvojem antivirusne Th1 i nepoželjne Th2 imunoreakcije. Th1 imunoreakcija je povezana s povišenom proizvodnjom IFN-γ te kemokina CXCL10 i CXCL9, dok je Th2 povezana s proizvodnjom IL-4 odnosno CCL17 i CCL22. U djece s akutnom RSV-infekcijom smo lokalno i sistemski odredili kemokinski profil obzirom na tip-1 i tip-2 imunoreakcije te ekspresiju specifičnih kemokinskih receptora (CXCR3 i CCR4) na T-limfocitima. Radi boljeg uvida u usmjeravanje diferencijacije T-limfocita u tip-2, određena je ekspresija CRTh2-receptora. Rezultati su pokazali da RSV specifično podiže razinu CXCL10 i CCL17 tijekom akutne infekcije. Neodgovarajući razvoj memorijske imunosti popraćen je povećanim postotkom TEM-limfocita tipa-1 i tipa-2, te izostankom razvoja dugoživućih memorijskih T-limfocita tipa-1. Nemogućnost razvoja odgovarajuće memorijske imunosti u kombinaciji s istovremenim razvojem oba tipa imunosnog odgovora, mogao bi igrati veliku ulogu u patogenezi RSV-a.- RSV (respiratory syncytial virus) is the most common cause of respiratory infections in infants. Development of severe lower respiratory tract infections and frequent reinfections could relate with the simultaneous development of protective Th1 and pathogenic Th2 immune responses. Th1 is represented by predominant IFN-γ and associated with increased CXCL10 and CXCL9 production. Contrary, Th2 is represented by increased IL-4 and CCL17/CCL22 production. We have determined the chemokine profile of children with severe RSV infection, in addition to specific chemokine receptors CXCR3 and CCR4 expression on memory T cells. To confirm type-2 T-cell polarization, we also analyzed CRTh2 receptor expression. Results showed RSV specifically increases CXCL10 and CCL17 levels in acute infection, followed by inadequate immune memory development in terms of higher percentages of type-1 and type-2 TEM cells, and lower percentages of long-living type-1 memory T cells. Failure in the development of appropriate immune memory, together with the presence of both type-1 and type-2 immune response, could play an important role in RSV pathogenesis and reinfections.- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
Provider: - Institution: - Data provided by Europeana Collections- Antitimocitni globulini su poliklona protutijela koja dovode do smanjenja broja T stanica. Često se koriste za imunosupresiju ...primatelja tijekom transplantacije bubrega. Timoglobulin su IgG protutijela dobivena imunizacijom zečeva timocitima ljudskog fetusa. Timoglobulin dovodi do smanjenja broja T-limfocita putem razgradnje stanica mehanizmom ovisnim o komplementu te apoptozom. Da bismo dokazali direktan učinak timoglobulina na stanice, koristili smo ljudske embrionalne stanice porijeklom iz bubrega (HEK-293) u staničnoj kulturi. Mjerili smo membranski potencijal stanice metodom prikovanih potencijala. Depolarizacijski učinak timoglobulina na membranski potencijal HEK-293 stanica je ovisan o koncentraciji timoglobulina te o početnom membranskom potencijalu stanica. Učinak timoglobulina u hipoksično-reoksigenacijskih uvjetima utvrđen je određivanjem stanične smrtnosti te stanične migracije testom grebanja. Timoglobulin je smanjio staničnu smrtnost u hipoksijskim uvjetima nakon kojih je slijedila reoksigenacija. Ovo je prvo istraživanje koje pokazuje direktan učinak timoglobulina na HEK-293 stanice. Rezultati ovog istraživanja potiču nova istraživanja o djelovanju timoglobulina na stanice transplantiranih bubrega.- Antithymocyte globulines are polyclonal T cell-depleting immunoglobulins used in induction of immunosuppression in kidney transplant recipients. Thymoglobuline, is purified rabbit IgG, obtained by immunization of rabbits with fetal human thymi, which depletes T lymphocytes by complement-dependent lysis and apoptosis. To determine possible direct effects of Thymoglobuline on the cells, we used Human Embryonic Kidney cell line (HEK-293) in culture. We measured membrane potential of the cells using slow whole patch clamp technique. Depolarizations of HEK-293 cells caused by Thymoglobuline were concentration- and membrane potential- dependent, showing direct effect of Thymoglobuline on the HEK-293 cells. The effects of Thymoglobuline in hypoxia/reoxigenation were detected by calculating cell death and determining the cell migration using scratch test. Thymoglobuline prevented the cell death induced by hypoxia and reoxigenation conditions. This is first study showing direct effect of thymoglobulin on HEK-293 cells. The results of this study encourage the research on epithelial cells in transplanted kidneys.- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
