In this study, response surface methodology (RSM) was used to optimize the extraction parameters of dandelion root polysaccharide (DRP). Two novel polysaccharides (DRP-2b, DRP-3a) were isolated from ...dandelion root by graded ethanol precipitation and column chromatography. Structural analyses indicated that DRP-2b, with a molecular weight of 31.8 kDa, was composed of rhamnose, glucuronic acid, glucose, galactose and arabinose, whereas DRP-3a, with a molecular weight of 6.72 kDa, was composed of rhamnose, glucose, galactose and arabinose. The backbones of DRP-2b and DRP-3a were mainly composed of (1 → 5)-linked-α-D-Ara and (1 → 6)-linked-α-D-Glc, respectively. In addition, the antioxidant activities and protective effects against H2O2-induced damage in hepatic L02 cells were evaluated. DRP-3a exhibited higher radical scavenging activity than DRP-2b against the DPPH, hydroxyl and superoxide anions. Furthermore, DRP-3a exhibited strong protective effects against H2O2-induced damage in hepatic L02 cells. Thus, DRP-3a could be used as a potential antioxidant in medicine or as a functional food.
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•RSM was successfully used for optimizing the extraction parameters of DRP.•Two new polysaccharides (DRP-2b, DRP-3a) were obtained from dandelion root.•Preliminary characterization of the two polysaccharides was performed.•DRP-3a possessed stronger free radical scavenging ability than DRP-2b.•DRP-3a exhibited protective effects against H2O2-induced damage in L02 cells.
Summary
The influence of dandelion root polysaccharide (DRP) on the retrogradation characteristics of corn starch (CS) in the process of storage was studied. Compared with native corn starch gel, the ...blue value (BV), hardness and syneresis were significantly decreased with the increasing of DRP concentration. Fourier transform infrared (FT‐IR) and X‐ray diffraction (XRD) showed that DRP addition inhibited the recrystallisation of CS during retrogradation. In addition, the dynamic rheological behaviours confirmed that DRP could enhance the shear stability and reduce the elastic properties of corn starch gel. These data showed DRP had potential application value in delaying starch retrogradation and improving food texture.
Gastric cancer (GC) is one of the most common tumors worldwide. Standard treatment after early detection involves surgical excision (recurrence is possible), and metastatic gastric cancer is ...refractory to immuno-, radio-, and most harmful chemotherapies. Various natural compounds have shown efficacy in killing different cancers, albeit not always specifically. In this study, we show that dandelion root extract (DRE) specifically and effectively suppresses proliferation and migration in human gastric cells without inducing toxicity in noncancerous cells. Long noncoding RNAs (lncRNAs) are known to promote tumorigenesis in many cancer types. Here, we showed that the lncRNA colon cancer-associated transcript-1 (CCAT1) was down-regulated in dandelion-treated GC cells. Furthermore, downregulation of CCAT1 inhibited proliferation and migration of gastric cells. We also found that DRE exerted its function in GC cells partially through targeting CCAT1. This data will provide a basis on which further research in cancer treatment through DRE can be executed.
Currently, scientists are increasingly focusing on utilizing the natural flora of the planet to search for and isolate individual bioactive substances that prevent various diseases, contribute to ...increased life expectancy, and affect all major life-supporting systems in the human body. This study describes the examination of the composition of plant raw materials from the Siberian Federal District. The research focuses on plant specimens from the root parts of
and
, collected in the Kemerovo region. The study determines the contents of the water-soluble vitamins B and C in the research subjects. The investigation includes assessing antioxidant properties, antimicrobial activity, and flavonoid content in extracts based on plant raw materials. All samples show a high percentage of antioxidant activity, with the highest antioxidant activity for
at 85.51 and that for
at 88.97. The results indicate low antimicrobial activity against
(growth inhibition zone up to 15.5 mm). Plant extracts contain significant amounts of B-group vitamins, with pyridoxine in
(156.40 μg/mL) and thiamine (46.20 μg/mL) and pyridoxine (357.10 μg/mL) in
. Flavonoids (rutin and quercetin) are identified in
and
extracts based on the study results.
In this study, purification, preliminary characterization and hepatoprotective effects of water-soluble polysaccharides from dandelion root (DRP) were investigated. Two polysaccharides, DRP1 and ...DRP2, were isolated from DRP. The two polysaccharides were α-type polysaccharides and didn't contain protein. DRP1, with a molecular weight of 5695 Da, was composed of glucose, galactose and arabinose, whereas DRP2, with molecular weight of 8882 Da, was composed of rhamnose, galacturonic acid, glucose, galactose and arabinose. The backbone of DRP1 was mainly composed of (1→6)-linked-α-d-Glc and (1→3,4)-linked-α-d-Glc. DRP2 was mainly composed of (1→)-linked-α-d-Ara and (1→)-linked-α-d-Glc. A proof-of-concept study was performed to assess the therapeutic potential of DRP1 and DRP2 in a mouse model that mimics acetaminophen (APAP) -induced liver injury (AILI) in humans. The present study shows DRP1 and DRP2 could protect the liver from APAP-induced hepatic injury by activating the Nrf2-Keap1 pathway. These conclusions demonstrate that the DRP1 and DRP2 might be suitable as functional foods and natural drugs in preventing APAP-induced liver injury.
Background: This study was conducted to investigate the effects of dandelion (Taraxacum officinale) root supplementation on performance, egg quality and some serum antioxidant enzymes capacity with ...increasing age, after the peak laying stage in hens. This study, also aimed to overcome diseases that occur due to lipid metabolism in layers in the late laying period. Methods: In total, one hundred and twenty 70-week-old Lohman LSL laying hens were randomly assigned to five groups equally (n = 24). Each treatment was replicated six times (6 replicates x 4 birds). The control group was fed basal diet (2650 kcal/kg ME) with no supplementation. The other groups were fed high-energy diets (2850 kcal/kg ME) to induce fatty liver and also 5, 10 and 15 g/kg dandelion (taraxacum officinale) root were supplemented. Result: The study revealed that the addition of 5 g/kg dandelion root to the high-energy feed positively changed the egg production, decreased lipid profiles (triglyceride and VLDL) and liver fat ratio and played a role in preventing lipid peroxidation.
Recently, due to the corrosion problem in the petroleum industry and the usage of commercial corrosion inhibitors, which, when released untreated into the environment, are considered to be ...environmentally unfriendly, green corrosion inhibitors are being researched. In this paper, the results of dandelion-root-extract testing as a green corrosion inhibitor for carbon steel in simulated brine solution saturated with carbon dioxide (CO2) are shown. The extract’s inhibition efficiency in static and flow conditions was determined by using potentiodynamic polarization with Tafel extrapolation and electrochemical-impedance-spectroscopy methods. In static conditions, the extract was tested at different concentrations. A maximum inhibition efficiency of 98.37% in static conditions at an extract concentration of 12 mL/L and 82.80% in flow conditions at a concentration of 14 mL/L was achieved. Additionally, for the most efficient dandelion-root-extract concentration (12 mL/L), the biodegradability and toxicity were determined. A biodegradability of 0.96 and a toxicity of 2.38% was achieved. Based on the obtained results of the conducted laboratory measurements, it can be concluded that dandelion-root extract has significant potential in terms of its use as a green corrosion inhibitor for carbon steel in a CO2-saturated brine solution.
Ground and unground dandelion roots were subjected to dielectric barrier discharge cold plasma (DBDCP) at 40 kV for 0 (control), 10, or 20 min. Then, infusions of the pretreated dandelion roots in ...water were prepared, and the changes in color, total phenolic content (TPC), antioxidant activity, and sensory properties were investigated. The 20‐min pretreatment increased the b* value, TPC, antioxidant activity, and sage odor of the ground dandelion root infusions compared with the control, whereas decreases in the TPC, antioxidant activity, and sage odor were noted in the 10‐min pretreated infusions of the unground roots. DBDCP pretreatment did not affect the overall likeliness of infusions of ground and unground roots. In addition, the TPC, antioxidant activity, and overall likeliness of infusions of the ground dandelion roots were higher than those of the unground samples. In conclusion, it can be said that the DBDCP pretreatment can be utilized to improve the TPC and antioxidant activity of ground dandelion roots.
Dandelion root samples were subjected to dielectric barrier discharge cold plasma, and the infusions in water were prepared. The changes in color, total phenolic content, antioxidant activity, and sensory properties were evaluated.
Dextran sodium sulfate (DSS)-triggered ulcerative colitis (UC) model in animals provides a valuable platform to preclinically evaluate the outcome of drug candidates for UC. Dandelion root extracts ...(DRE) have a therapeutic effect on UC. However, the protective mechanism of DRE against UC remains unknown.
To discover the targeting pathway involved in DRE-induced protection against UC.
The UC model was developed in C57BL/6 mice by oral administration of DSS. Following DSS exposure, sulfasalazine (SASP), low dose of DRE (DRE-L), moderate dose of DRE (DRE-M), high dose of DRE (DRE-H), and DRE-H plus mitogen-activated protein kinases (MAPK) agonist (DRE-H+MA) were administered to the mice. Colon Mucosal Damage Index (CMDI) and histopathological analysis were used to evaluate the colonic mucosal damage. The cytokine levels were detected using commercial enzyme-linked immunosorbent assay (ELISA) kits. The MAPK pathway activation was determined with western blotting.
We found that DRE-H attenuated DSS-triggered colonic mucosal damage. The DSS-induced inflammatory responses and oxidative stress in the bloodstream and colon tissues were dramatically inhibited by DRE-H administration. Also, this plant impaired DSS-provoked phosphorylation levels of extracellular signal-regulated kinases (ERK), c-Jun N-terminal kinases (JNK), p38 mitogen-activated protein kinases (p38), p65, and IκB. More importantly, MAPK agonist, BIM-23A760, removed the protective effect of DRE-H on the bloodstream and colon tissues.
The DRE-H is capable of relieving DSS-induced UC, and its mechanism links to the MAPK pathways.
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