La variété des styles des illustrations est a souligner, allant de dessins aux traits simples, pour évoquer des souvenirs d'enfance par exemple (43-47), a des illustrations plus abstraites, tres ...sombres et plus chargées, pour rendre compte de pans de l'histoire associés a des violences et des souffrances - notamment ce qui concerne les pensionnats autochtones (150-151). Les Premiers Peuples n'existaient alors pour elle et son entourage qu'a travers « les jeux de cowboys, les bandes dessinées, les western-spaghetti et les costumes d'Halloween » (46). Le quatrièe chapitre aborde les enjeux et les événements qui ont participé à un éveil, d'abord personnel, pour l'autrice, mais aussi celui plus global, dans la société, aux réalités autochtones et a la perpétuation des violences coloniales jusqu'à nos jours (101).
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•AaMYB113 exhibits strong transcriptional activity, in contrast to AaMYB114, which demonstrates minimal activity.•AaMYB113 induced red pigmentation in tobacco and Arabidopsis, whereas ...AaMYB114 did not.•AaMYB113 promotes the expression of anthocyanin biosynthesis-related genes in tobacco and Arabidopsis.•AaMYB113 activates the promoter activity of structural anthocyanin biosynthesis genes from Aeonium arboreum ‘Halloween’.
Aeonium arboreum ‘Halloween’, a popular indoor ornamental succulent in China, changes its leaf colour to red on light exposure. However, the underlying molecular mechanisms is still vague. Comparative analysis of transcriptome data from ‘Halloween’ leaves treated under dark and light conditions revealed two R2R3-MYB transcription factors, AaMYB113 and AaMYB114, that may mediate anthocyanin accumulation. In this study, we cloned the AaMYB113 and AaMYB114 genes, encoding proteins of 279 and 248 amino acids, respectively. Transcriptional activity analysis revealed that AaMYB113 exhibits strong transcriptional activity, in contrast to AaMYB114, which demonstrates minimal activity. Transient expression studies in tobacco leaves demonstrated that AaMYB113 induced red pigmentation, whereas AaMYB114 did not. Subsequent stable overexpression in Arabidopsis thaliana confirmed that AaMYB113, but not AaMYB114, could similarly turn Arabidopsis leaves red. Further stable transformation of AaMYB113 in tobacco affected multiple floral components, including leaves, petals, calyx, flower tubes, and filaments, turning them red. Quantitative real-time PCR (qRT-PCR) assay in leaves of AaMYB113 stably transformed tobacco and Arabidopsis revealed upregulation of anthocyanin biosynthesis-related structural genes and TT8-like transcription factors. Moreover, the dual luciferase analysis confirmed that AaMYB113 can activate the promoters of ‘Halloween’ anthocyanin synthesis structural genes, AaCHS, AaCHI, AaF3H, AaDFR and AaANS. The above results indicate that AaMYB113 can promote anthocyanin synthesis, while AaMYB114 does not have this function. This study contributes significantly to the limited body of research on the molecular mechanisms of anthocyanin synthesis in succulents, advancing our understanding of how these pathways are regulated in ‘Halloween’ succulents and potentially other species.
