The present study reports the green synthesis of pectin-fabricated silver nanocomposites (Pectin-AgNPs) using Carpesium nepalense leaves extract, evaluating their bactericidal kinetics, in vivo ...hepatoprotective, and cytotoxic potentials along with possible mechanisms. GC/MS and LC/MS analyses revealed novel phytochemicals in the plant extract. The Pectin-AgNPs were characterized using UV/Vis, AFM, SEM, TEM, DLS, FTIR, and EDX techniques, showing a spherical morphology with a uniform size range of 50–110 nm. Significant antibacterial activity (P < 0.005) was found against four bacterial strains with ZIs of 4.1 ± 0.15 to 27.2 ± 3.84 mm. AFM studies revealed significant bacterial cell membrane damage post-treatment. At 0.05 mg/kg, the nanocomposites showed significant (P < 0.005) hepatoprotective activity in biochemical and histopathology analyses compared to the CCl4 control group. Pectin-AgNPs significantly reduced (P < 0.005) LDH, AST, ALT, ALP, and DB levels. qPCR analysis showed ameliorative effects on PPARs and Nrf2 gene expression, restoring gene alterations caused by CCl4 intoxication. In vivo acute toxicity studies confirmed low toxicity of Pectin-AgNPs in major organs. Pectin-AgNPs exhibited cytotoxic activity against HeLa cell lines at higher doses with an LC50 of 223.7 μg/mL. These findings demonstrate the potential of Pectin-AgNPs as promising antibacterial, hepatoprotective, and cytotoxic agents.
•Synthesized Pectin-AgNPs via green route using Carpesium nepalense.•GC/MS & LC/MS revealed new phytochemicals in the plant extract.•Significant antibacterial activity against four bacterial strains.•Exhibited hepatoprotective effects in biochemical, histopathology, qPCR, and western blotting.•Showed low in-vivo toxicity and cytotoxicity against HeLa cells at high doses.
•Temperature, pH value, substrate content affected the inhibition of pectin on AA content.•Pectin addition inhibited AA formation by lowering the pH value in Asn/Glc model system.•The competition ...between pectin degradation product and Glc for Asn resulted in AA inhibition.
Acrylamide (AA) is a potential carcinogen formed during the process of food heating. Pectin is natural food additive widely presented in fruits and vegetables. This study aimed at investigating the influence of the addition of high methoxyl apple pectin (esterification degree: 82.6%) on AA inhibition in the asparagine (Asn)/glucose (Glc) model system. Results showed that temperature (120–180 °C), pH value (6.0–7.2), pectin addition (0.2–1.0%, w/v), substrate concentration (0.01–0.5 M) and molar ratio of Asn/Glc (5:1–1:10) had significant influence on inhibition of pectin on AA formation. With adding 1.0% (w/v) pectin, the pH value, Glc consumption and Schiff base abundance declined in Asn/Glc model system. Moreover, heating treatment decreased the pH value, molecular weight, esterification degree and galacturonic acid content of pectin. Finally, the pectin degradation product was identified, which might compete with Glc for Asn in Maillard reaction, led to AA reduction. This study provided distinct evidence for controlling AA formation.
Summary
To improve long‐term stability of the liposomes, resveratrol (RES) liposomes, epigallocatechin gallate (EGCG) liposomes, RES+EGCG liposomes, RES+EGCG liposomes coated with low methoxyl pectin ...(LMP) were prepared, and their physical properties were evaluated. The prepared liposomes have a particle sizes of about 110–160 nm. The LMP‐coated composite liposomes have the largest particle size and zeta potential, the latter indicating higher system stability. Liposome systems were pasteurised and placed in an orange juice system as to study liposome stability in a real the food environment. LMP bridge with metal ions forming a network‐like gel in the LMP‐coated composite liposomes, thereby enhancing the stability of liposomes. LMP‐decorated liposomes could be developed as a formulation for encapsulating and delivering functional ingredients.
To improve long‐term stability of the liposomes, resveratrol (RES) liposomes, epigallocatechin gallate (EGCG) liposomes, RES+EGCG liposomes, RES+EGCG liposomes coated with low methoxyl pectin (LMP) were prepared, and their physical properties were evaluated. Compared to other liposomes, LMP‐coated complex liposome was more stable and have greater antioxidant effects after pasteurization, and maintain the best sustained release efficiency.
The mesocarp (albedo) of passion fruit is considered a waste product but rich in soluble fibers, especially pectins. Biological activity and health benefits of pectins have recently emerged, ...especially in colorectal cancer and attenuating inflammation. Pectin conventional extraction often uses mineral acids, which can be hazardous to the environment, and alternatives can be costly. Here, we assessed a high-temperature and pressure method to extract pectin from the passion fruit albedo and evaluated the differences from the water-soluble fractions extracted. HPSEC, HPAEC, FTIR-ATR, and HSQC-NMR were performed to identify and confirm the highly methylated homogalacturonan structures. The heat-modified samples showed a decreased molecular size compared to the untreated sample. Colorectal cancer cell lines showed reduced viability after being treated with different doses of modified samples, with two of them, LW-MP3 and 4, showing the most potent effects. All samples were detected inside cells by immunofluorescence assay. It was observed that LW-MP3 and 4 upregulated the p53 protein, indicating cell-cycle arrest and the cleaved caspase-9 in one of the cell lines, with LW-MP4 enhancing cell death by apoptosis. Since the modified samples were composed of hydrolyzed homogalacturonans, those probably were the responsible structures for these anti-cancer effects.
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•Drying of okra pod affected the properties of okra pectic polysaccharides (OP).•The type of drying did not affect the degree of methyl esterification of OP.•Drying at higher ...temperature decreased OP molecular weight and viscosity in solution.•Drying at higher temperature decreased the emulsifying stability of OP.•Drying at higher temperature increased OP oil absorption and antioxidant capacities.
This work investigates the effect of drying okra pods by different techniques freeze-drying (FD), sun-drying (SD), oven-drying (OD) and microwave-drying (MD) on the molecular structure, physicochemical and antioxidant properties of the subsequently extracted OP. Remarkably, although the degree of methyl esterification (∼41.1 %) remained similar among samples, the content of galacturonic acid (62.67–68.77 %), average number molecular weight (MnI: 758.8–808.5 kDa, MnII: 20.9–24.2 kDa), and to a greater extent the apparent viscosity of an aqueous solution of pectin molecules, water holding capacity (0.21–10.71 g/g) and emulsifying activity (42.3–72.7 %) and stability (38.6–53.5 %), decreased with the drying temperature in the order of FD-OP > SD-OP > OD-OP > MD-OP. On the other hand, only FD-OP presented a higher proportion of galactan and/or arabinan side chains (Ara + Gal) / Rha = 12.37% compared to the rest of the samples, with values ranging from 7.79 to 9.17%. FD-OP and SD-OP resulted in lower DPPH and ABTS radical scavenging activities.
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Piperine (PIP) is a herbal drug with well-known anticancer activity against different types of cancer including hepatocellular carcinoma. However, low aqueous solubility and extensive ...first-pass metabolism limit its clinical use. In this study, positively charged PIP-loaded nanostructured lipid carriers (PIP-NLCs) were prepared via melt-emulsification and ultra-sonication method followed by pectin coating to get novel pectin-coated NLCs (PIP-P-NLCs) targeting hepatocellular carcinoma. Complete in vitro characterization was performed. In addition, cytotoxicity and cellular uptake of nanosystems in HepG2 cells were evaluated. Finally, in vivo anticancer activity was tested in the diethylnitrosamine-induced hepatocellular carcinoma mice model. Successful pectin coating was confirmed by an increased particle size of PIP-NLCs from 150.28 ± 2.51 nm to 205.24 ± 5.13 nm and revered Zeta potential from 33.34 ± 3.52 mV to −27.63 ± 2.05 mV. Nanosystems had high entrapment efficiency, good stability, spherical shape, and sustained drug release over 24 h. Targeted P-NLCs enhanced the cytotoxicity and cellular uptake compared to untargeted NLCs. Furthermore, PIP-P-NLCs improved in vivo anticancer effect of PIP as proved by histological examination of liver tissues, suppression of liver enzymes and oxidative stress environment in the liver, and alteration of cell cycle regulators. To conclude, PIP-P-NLCs can act as a promising approach for targeted delivery of PIP to hepatocellular carcinoma.
Pectin is a valuable product that can be extracted from waste fruit peels. Here we propose the use of graphene oxide (GO)-based membranes for pectin concentration. The synthesized GO was ...functionalized with ethylenediamine (EDA) to molecularly design the GO framework. Kaolin hollow fibers with asymmetric pore distribution were used as a porous substrate for GO/EDA deposition. A GO/EDA layer with a thickness of 2.86 ± 0.24 μm was assembled on the substrate by the simple vacuum-assisted deposition method. After GO/EDA depositions, the water permeance of the pristine kaolin hollow fibers reduced from 8.46 ± 0.17 to 0.52 ± 0.03 L h
·m
·kPa
. A pectin aqueous extract from orange peels was filtered at cross-flow mode through the prepared membranes and the steady-state fluxes through pristine and GO/EDA-coated hollow fibers were 56 ± 2 and 20 ± 3 L h
m
, respectively. The GO/EDA-coated membrane presented greater pectin selectivity than the pristine hollow fiber. The GO/EDA-coated hollow fiber concentrated the galacturonic acid, phenolic, and methoxyl contents in 19.5, 17.4, and 29.2 %, respectively. Thus, filtration through the GO/EDA-based membrane is a suitable alternative for pectin concentration.
Fruit and vegetables are a great source of nutrients and have numerous health benefits. The fruit and vegetable industry produces enormous amounts of waste such as peels, seeds, and stems. The amount ...of this waste production has increased, causing economic and environmental problems. Fruit and vegetable wastes (FVWs) have the potential to be recovered and used to produce high-value goods. Furthermore, FVWs have a large variety and quantity of polysaccharides, which makes them interesting to study for potential industrial use. Currently, the investigations on extracting polysaccharides from FVWs and examining how they affect human health are increasing. The present review focuses on polysaccharides from FVWs such as starch, pectin, cellulose, and inulin, and their various biological activities such as anti-inflammatory, anti-tumor, anti-diabetic, antioxidant, and antimicrobial. Additionally, applications as packaging material, gelling agent, emulsifier, prebiotic, and fat replacer of polysaccharides from FVWs in the food industry have been viewed in detail. As a result, FVWs can be reused as the source of polysaccharides, reducing environmental pollution and enabling sustainable green development. Further investigation of the biological activities of polysaccharides from FVWs on human health is of great importance for using these polysaccharides in food applications.
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•Polysaccharides are important active components in fruit and vegetable wastes.•Polysaccharides from FVWs can be used for various food applications.•Fruit and vegetable polysaccharides show antioxidant and antimicrobial activities.•Fruit and vegetable wastes are rich sources of polysaccharides.
We previously found that modified citrus pectin (MCP), an inhibitor of pro-inflammatory factor Galectin-3 (Gal-3), has significant anti-inflammatory and chondroprotective effects. In this study, a ...hyaluronate (HA) gel-based sustained release system of MCP (MCP-HA) was developed as an anti-inflammatory agent for chronic inflammation for osteoarthritis (OA) treatment. The MCP-HA gel was injected into the knee joint cavities of OA rabbit models induced by anterior cruciate ligament transection (ACLT) or modified Hulth method once a week for five weeks. We found that MCP-HA could improve the symptoms and signs of OA, protect articular cartilage from degeneration, suppress synovial inflammation, and therefore alleviate OA progression. Proteomic analysis of the synovial fluid obtained from the knee joints of OA rabbits revealed that MCP-HA synergistically regulated the levels of multiple inflammatory mediators and proteins involved in metabolic pathways. Taken together, our results demonstrate that the MCP-HA shows a synergistic effect of HA and MCP by modulating both inflammation and metabolic processes, thereby alleviating OA progression. The MCP-HA sustained release system has promising potential for long-term use in OA treatment.
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•Injectable modified citrus pectin and hyaluronate gel (MCP-HA) was prepared.•MCP-HA could be used in local delivery and release MCP in a sustained way.•MCP-HA could alleviate osteoarthritis (OA) progression, had potential in OA treatment.•MCP-HA could regulate both inflammatory and metabolic pathways in OA treatment.
β-ionone has a unique violet odor and good biological activity, which is an essential fragrance component and potential anticancer drug. In this paper, β-ionone was encapsulated using complex ...coacervation of gelatin and pectin, followed by cross-linking with glutaraldehyde. The pH value, wall material concentration, core-wall ratio, homogenization conditions, and curing agent content were investigated in the single-factor experiments. For example, the encapsulation efficiency increased with the homogenization speed, which reached a relatively high value at 13000 r/min for 5 min. The gelatin/pectin ratio (3:1, w/w) and pH value (4.23) significantly affected the size, shape, and encapsulation efficiency of the microcapsule. The fluorescence microscope and SEM were used to characterize the morphology of the microcapsules, in which the microcapsule has a stable morphology, uniform size, and spherical multinuclear structure. FTIR measurements confirmed the electrostatic interactions between gelatin and pectin during complex coacervation. Thermogravimetric analysis (TGA) revealed that the microcapsules could maintain good thermal stability over 260 °C. The release rate of β-ionone microcapsule was only 20.6 % after 30 days at the low temperature of 4 °C. These findings provide an effective carrier to deliver flavors like β-ionone and could be useful in the fields of daily chemicals and textiles.
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