In the present study, new Schiff bases derived from pentafluorophenyl-hydrazine (L1, L2, L3, L4) were synthesized and their structures were characterized by
H nuclear magnetic resonance spectroscopy ...(NMR),
C NMR,
F NMR, fourier transform infrared spectroscopy, and elemental analysis methods. Then, the anticancer activities of the obtained compounds were investigated using three human cancer cell lines (A2780, over; Caco-2, colon; and HT-29 colon carcinoma cell lines). According to the obtained cytotoxicity results, compound number L4 was found to have the highest anticancer activity in A2780 (over) and Caco-2 (colon) cell lines. Furthermore, in silico, ADMET properties, where studies play an important role in the development and prediction of drug compounds, were calculated using web-based platforms. In addition, molecular docking studies were performed to evaluate the binding interactions between the synthesized pentafluorophenyl-hydrazone compounds and the MDM2 protein (4JSC). Both in vitro and in silico results showed that the synthesized compounds could act as potent anticancer agents.
A sensitive and reliable method has been developed for the simultaneous determination of 20 airborne carbonyl compounds in the C
1–C
10 range. The carbonyls were collected onto solid sorbent coated ...with pentafluorophenyl hydrazine (PFPH), followed by solvent extraction and gas chromatographic (GC)/mass spectrometric (MS) analysis of the PFPH derivatives. The sorbent is packed into two separate sections in a glass sampling tube. The two-section design allows convenient checking of collection efficiency and breakthrough. The sampling tube, with a coating amount of 971
nmol PFPH per 100
mg Tenax TA and operated at a sampling flow rate of 80
mL
min
−1, collects the 20 carbonyls with efficiencies above 95%. Hexane extracts the collected carbonyls in their PFPH derivatives in the sampling tube with better than 95% extraction efficiency. It is necessary to let the sampling tube sit at ambient temperature for 3 days before solvent extraction to ensure complete derivatization of the carbonyls. The limits of detection (LODs) of the tested carbonyls are in the range of 3.7–11.6
ng per sample. The method has been field-tested both in ambient environment and in an indoor environment from burning mosquito-repellent incense. Eighteen carbonyls were detected in the ambient air samples with the exception of
o-tolualdehyde and
m-tolualdehyde, while all the 20 target carbonyls were found in the incense smoke. Compare field test with classical DNPH–HPLC/UV method, good agreement exited between the two methods for lower molecular carbonyls but PFPH method is found to be a better analytical method for determination of high molecular weight carbonyls.
In this contribution, bar adsorptive micro-extraction using polystyrene-divinylbenzene sorbent phase and in situ derivatization with pentafluorophenyl hydrazine, followed by liquid desorption and ...high-performance liquid chromatography-diode array detection (BAμE(PS-DVB)PFPH in situ-LD/HPLC-DAD), was developed for the determination of six short-chain carbonyl compounds (formaldehyde, acetaldehyde, propanal, acetone, butanone, and 2-hexenal) in drinking water matrices. PFPH presented very good specificity as an in situ derivatization agent for short-chain ketones and aldehydes in aqueous media, allowing the formation of adducts with remarkable sensitivity, selectivity and the absence of photodegradation. Assays performed on 30-mL water samples spiked at the 25.0 μg L⁻¹ levels, under optimized experimental conditions, yielded recoveries ranging from 47.4 ± 3.8% to 85.2 ± 3.8%, in which the PS-DVB proved to be a convenient sorbent phase. The analytical performance showed good accuracy, suitable precision (RSD < 13.0%), detection limits in between 47 and 132 ng L⁻¹ and remarkable linear dynamic ranges (r ² > 0.9907) from 1.0 to 80.0 μg L⁻¹. By using the standard addition methodology, the application of the present method to drinking water samples treated with different disinfectants, namely, chloride, ozone and both, allowed very good performances to monitor these priority compounds at the trace level. The proposed methodology proved to be a feasible alternative for polar compound analysis, showing to be easy to implement, reliable, sensitive and requiring a low sample volume to monitor short-chain aldehydes and ketones in drinking water matrices.
Solid-phase microextraction (SPME) in conjunction with isotope dilution mass spectrometry (ID-MS) was employed for the analysis of formaldehyde in cosmetic products. The formaldehyde is derivatized ...in situ with pentafluorophenyl hydrazine. The formed hydrazone is adsorbed over a poly(dimethylsiloxane)–divinylbenzene-coated fiber and analyzed using gas chromatography–mass spectrometry. The adsorption–time profiles and salting effect were studied. The quantitation was performed by using a stable isotope labeled analogue as an internal standard. The precision, recovery and detection limits were determined with spiked samples. The relative standard deviations from different spiked cosmetic samples were all less than 10% and the recoveries were between 89.00 and 101.23%. The limit of detection was of 0.39
μg/l. Compared with other techniques, the study shown here provides a simple, fast and reliable method for the analysis of formaldehyde in cosmetic products.
A method for determining carbonyl emissions from motor vehicles was developed in which exhaust gases from test vehicles running on a dynamometer are directly collected in 6-L Teflon bags and then ...introduced into pentafluorophenyl hydrazine -coated adsorbent tubes for derivatisation and subsequent analysis of generated derivatives by GC/MS. Twenty gasoline and LPG light-duty vehicles were sampled at a vehicle inspection station in Guangzhou, China, by running test vehicles over a transient testing cycle and analysed for carbonyls. Results show that the method can achieve a detection limit of 8-28 ng m
−3
for individual carbonyls with a relative standard deviation of 14.5%. Twenty-four carbonyls were identified in the gasoline vehicle exhaust gases and their total concentration was 19.2 mg m
−3
. For the LPG vehicles, 20 carbonyls were found in the exhaust gases with a total concentration of 4.71 mg m
−3
. Based on the results, carbonyl emission factors for the gasoline and LPG vehicles were estimated to be 25.0 and 7.36 mg km
−1
, respectively.
A sensitive and reliable method for detection of irradiated chicken, pork and mangoes is described. The method involved a derivatisation treatment of irradiation markers--2-dodecylcyclobutanone ...(2-dDCB) and 2-tetradecylcyclobutanone (2-tDCB) with pentafluorophenyl hydrazine (PFPH) and detection by gas chromatography-mass spectrometry (GC-MS). Samples were first subjected to Soxhlet extraction with n-hexane and purified by passing through Florisil columns according to EN1785 method. Then, the extracted 2-dDCB and 2-tDCB were derivatised with PFPH in acidic buffer for 60 min at ambient temperature (25 °C). Identification of the derivatives was confirmed by the presence of two characteristic and dominant ion fragments at m/z 249 and M-54, each within the specified retention time windows, and matching of the relative intensities of these ions with those of the standards under SIM mode. The relative standard deviation (%RSD) of the ion ratios of m/z 249 to that of M-54 for all the food matrices under study (2.96-3.06 for PFPH-dDCB; and 2.69-2.83 for PFPH-tDCB, respectively) were ≤10%. Mean spike recovery of the derivatization process, with %RSD ≤20%, ranged from 68.6% to 71.4% and 57.9% to 70.8% for PFPH-dDCB and PFPH-tDCB respectively. The limits of detection (LODs) were estimated to be 0.01 μg/g. The present method was applicable to detect food samples that had received irradiation doses at 1-5 kGy. The quantity of equivalent 2-dDCB and 2-tDCB in the concerned foods was found to be proportional to the irradiated dose received.PUBLICATION ABSTRACT
