Karyotypic analysis and cellular DNA contents of Pseudorasbora parva Yang Kun, Huazhong Agricultural University, Wuhan (China), College of Fisheries; Wang Zijian, Chinese Academy of Sciences, Beijing (China), Research Center for Eco-environmental Sciences; Zhu Dongmei, Huazhong Agricultural University, Wuhan (China), College of Fisheries
Huazhong nongye daxue xuebao,
Jun. 2012, Letnik:
31, Številka:
3
Journal Article
采用PHA和秋水仙素活体注射、肾细胞短期培养和空气干燥制片法, 对麦穗鱼染色体数目和核型进行研究; 以麦穗鱼外周血细胞为样本, 鸡血细胞DNA为标准(2.30pg), 使用美国贝克曼库尔特公司Cell LabQuanta SC流式细胞仪测定了麦穗鱼二倍体细胞的DNA含量。结果显示：麦穗鱼染色体数目2n=50, 未观察到次缢痕及性染色体, 亦未发现有随体, 核型公式为18m＋22sm＋10st, ...NF=90; 麦穗鱼二倍体细胞DNA含量为鸡血对照的1.38倍, 绝对含量为3.18pg。
The chromosome number and karyotype of Pseudorasbora parva were analyzed using a routine method including intraperitoneal injection of PHA and colchicine, cultivation in vivo, and slides preparation with air-drying. Whilst erythrocyte nuclear DNA content of P.parva was determined by using a flow cytometer(Beckman Coulter Inc., USA) with serving the chicken erythrocytes(their DNA content is 2.30 pg) as reference cells. Results obtained in this study showed that：the diploid chromosome number of P.parva was 50(2n=50); the karyotype of this species was 2n=18 m＋22 sm＋10 st, NF=90, while the secondary constriction, heterosome and satellite chromosome were not observed; the diploid cellular DNA contents of P.parva was 3.18 pg, which was 1.38 times to the chicken erythrocytes.
