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  • Cell cycle regulating genes and their protein expression in squamous cell carcinoma of the larynx and hypopharynx = Spremembe genov in proteinov celičnega ciklusa pri ploščatoceličnem karcinomu grla in spodnjega žrela
    Volavšek, Metka ; Glavač, Damjan ; Gale, Nina
    Background. The major mechanisms involved in genomic instability during tumourprogression are loss of heterozygosity (LOH) and microsatellite instability (MSI). The most frequently affected are the ... tumor suppressor genes(TSG). Alterations of cell cycle proteins contribute to the development and biologic behaviour of malignant tumours. Methods. In a prospective study we evaluated the distribution and prognostic significance of immunohistochemically detected proteins p53, p21, p16, Rb, and cyclin D1 in 101 squamous cell carcinomas of the larynx and hypopharynx (LHSCC). Additionally, non isotopic MSI and LOH analysis was performed with microsatellite markers on chromosomes 3p, 9p, 17p, and 11q. Immunohistochemical and molecular alterations were compared to tumour grade, disease stage and three year patients overall and disease free survival. Results. Of 101 patients, there were 94 men and 7 women with 73 laryngeal and 28 hypopharyngeal cancers. Immunohistochemical staining was performed on all tumours and molecular analysis in 77 patients. In LHSCC, varying patterns of protein expression were found. A significant correlation was found between cyclin D1 and p21, cyclin D1 and Rb expression, and Rb expression and tumour grade. p53 and p16 expression did not correlate with other proteins. p16 expression correlated with LOH at 9p21, and LOH at 11q13 (cyclin D1 region) correlated with the tumour grade. We observed a high incidence of LOH at specific chromosomal regions: 3p (61%), 9p (54.4%), 17p (57.1%) and 11q (19.5%). Conversely, MSI was present in 6.5% of cases. In addition to tumour grade and N stage, only cyclin D1 expression revealed independent prognostic value for overall, but not disease free survival after multivariate analysis. Conclusions. In conclusion, our studydemonstrated the derailment of the growthpromoting and suppressing pathways of cell cycle control in almost all LHSCC. (Abstract truncated at 2000 characters).
    Vir: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 71, suppl. 3, november 2002, str. III-29 - III-34)
    Vrsta gradiva - članek, sestavni del
    Leto - 2002
    Jezik - angleški
    COBISS.SI-ID - 15695577

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