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Optimisation of n-octyl oleate enzymatic synthesis over Rhizomucor miehei lipaseLaudani, Chiara Giulia ...Octyl oleate is a useful organic compound with several applications in cosmetic, lubricant and pharmaceutical industry. At first, the enzymatic synthesis of n-octyl oleate by direct lipase-catalysed ... esterification of oleicacid and 1-octanol was investigated in a stirred batch reactor in solvent-free system. A systematic screening and optimisation of the reaction parameters were performed to gain insight into the kinetics mechanism. Particularly, enzyme concentration, reaction temperature, stirrer speed, watercontent, substrates concentration and molar ratio were optimised with respect to the final product concentration and reaction rate. The kinetics mechanism of the reaction was investigated. Finally, a comparison of the experimental results obtained in a solvent free-system with those using two different solvents, supercritical carbon dioxide (SC-CO2) and n-hexane, was proposed. It resulted that in SC-CO2 higher concentration of the desired product was attained, requiring lower enzyme concentrations to achieve comparable conversion of free fatty acid into fatty acid ester.Vir: Bioprocess and biosystems engineering. - ISSN 1615-7591 (Vol. 29, no. 2, 2006, str. 119-127)Vrsta gradiva - članek, sestavni delLeto - 2006Jezik - angleškiCOBISS.SI-ID - 10569494
Avtor
Laudani, Chiara Giulia |
Leitgeb, Maja |
Primožič, Mateja, 1975- |
Knez, Željko |
Della Porta, Giovanna |
Reverchon, Ernesto
Teme
kemijska procesna tehnika |
superkritični fluidi |
CO2 |
lipaze |
encimi |
topila |
n-oktil oleati |
hitrost reakcije |
chemical processing |
supercritical fluids |
CO2 |
lipase |
enzymes |
solvents |
n-octyl oleate |
kinetic study
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Laudani, Chiara Giulia | ![]() |
Leitgeb, Maja | 00534 |
Primožič, Mateja, 1975- | 21460 |
Knez, Željko | 02619 |
Della Porta, Giovanna | ![]() |
Reverchon, Ernesto | ![]() |
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