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  • Optimization of cultivation conditions in spin tubes for CHO cells producing erythropoietin
    Strnad, Jure, 1982- ...
    This paper describes the optimization of cultivation factor settings, i.e. theshaking rate and working volume in 50 mL spin tubes for a Chinese hamster ovary (CHO) cell line expressing recombinant ... human erythropoietin, using a response D-optimal surface method. The main objectives of the research were, firstly, to determine a setting in which the product titer and product qualityattributes in spin tubes are equivalent to those in 250 mL shake flasksin a seven day batch and, secondly, to find a setting in which the product titer is maximal. The model for product titer prediction as a functionof shaking rate and working volume in the defined design space was successfully applied to the optimization of cultivation conditions in spin tubes for the tested cell line. Subsequently, validation experiments were carried out simultaneously in spin tubes, shake flasks and bench scale bioreactors in order to compare cell culture performance parameters such as growth, productivity and product quality attributes in the form of isoform profiles and glycan antennarity structures. The results of the experiments showed that similar cell culture performance and product quality could be achieved in spin tubes when compared to shake flasks. Additionally, bioreactortiters could be reproduced in spin tubes at high shaking rates and low working volumes, but with differing product quality. Cultivation at lower shaking rates in spin tubes and shake flasks produced a glycoprotein with a product quality slightly comparable to that from bioreactors, but with titers being only two thirds.
    Vir: 20th European Symposium on Computer Aided Process Engineering : [ESCAPE-20, 6th to 9th June 2010, Ischia, Italy] (Str. 235-240)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2010
    Jezik - angleški
    COBISS.SI-ID - 14224406

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