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  • Detecting various bacteria on metal and glass surfaces [Elektronski vir]
    Fijan, Sabina ; Murko, Patricija ; Šostar-Turk, Sonja
    Although person-to-person transmission is the most likely route for healthcare associated infections, the role of the environment should not be ignored and may also contribute to the spread of ... nosocomial infections. It has been proven that microorganisms are able to survive on environmental surfaces up to several weeks, therefore providing a potential for biotransfer. Assessment of environmental cleanliness is mainly conducted by surface sampling and swabbing, which is followed by classical incubation methods based on phenotypic detection of microorganisms. These methods take between 2 to 4 days. Novel quick methods based on DNA detection using PCR methods is a possibility for quick detection of the presence of DNA. In the present study, we investigated the presence of the following challenge microorganisms: Enterococcus faecium, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa on metal and glass surface after 24 hour drying using classical incubation methods and PCR methods. We detected all challenge microorganisms at initial concentrations as low as 102 cfu/mL and final concentration after drying at 30 cfu/mL. PCR methods proved to be equal or more sensitive compared to classical incubation methods for all challenge microorganisms.
    Vir: Proceedings 2015 [Elektronski vir] (Str. 1431-1436)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2015
    Jezik - angleški
    COBISS.SI-ID - 2111652

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