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Araújo, Isaac Jordão de Souza; Guimarães, Gustavo Narvaes; Machado, Renato Assis; Bertassoni, Luiz Eduardo; Davies, Robert Philip Wynn; Puppin-Rontani, Regina Maria
Journal of dentistry, 06/2022, Letnik: 121Journal Article
Self-assembling peptide P -4 is amphiphilic and pH-triggered, effective on repairing early enamel carious lesions and dentin remineralization. However, P -4 effects on dentin biomineralization and repair ability remain unexplored. Thus, cytocompatibility and effectiveness of P -4 on inducing mineralization and migration of odontoblast-like cells (MDPC-23) were investigated. MDPC-23 were seeded in contact with P -4 (0.5 and 1 µg/ml), Dentin Matrix Protein 1 (DMP1 0.5 and 1 µg/ml) or Calcium hydroxide (Ca(OH) 100 µg/ml) solutions. Cell viability was verified using MTT (n = 6/group). Mineral deposition was tested using Alizarin Red (n = 4/group). Cell migration was assessed by light microscopy (n = 2/group). MTT and Alizarin Red data were compared using Kruskal-Wallis and Mann-Whitney (α=0.01). P -4 (0.5 and 1 µg/ml) and DMP1 (0.5 and 1 µg/ml) resulted the highest cell viability; Ca(OH) presented the lowest. 1 µg/ml DMP1 and 1 µg/ml P -4 promoted the highest mineral deposition. Ca(OH) presented lower values of mineral deposits than DMP1 1 µg/ml (p < 0.01), but similar to P -4 1 µg/ml. P -4 and DMP1 at 0.5 µg/ml induced lesser mineral precipitation than P -4 and DMP1 at 1 µg/ml (p < 0.01), with no difference to Ca(OH) . All materials stimulated cell migration, however, lower concentrations of DMP1 and P -4 demonstrated a higher migration potential. P -4 did not affect cell viability, induces mineral deposition and MDPC-23 migration like DMP1. Self-assembling peptide P -4 does not affect the cell viability and induces mineral deposition comparable to native protein involved in biomineralization. Combined with its ability to bind type I collagen, P -4 is a promising bioinspired molecule that provides native-tissue conditions and foster further studies on its ability to form dentin bridges in pulp-capping strategies.
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Vir: Osebne bibliografije
in: SICRIS
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