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Chen, Lichan; Zeng, Xiaoting; Si, Peng; Chen, Yingmei; Chi, Yuwu; Kim, Dong-Hwan; Chen, Guonan
Analytical chemistry (Washington), 05/2014, Letnik: 86, Številka: 9Journal Article
Two-dimensional graphite-like carbon nitride nanosheets (g-C3N4 NSs) were hybridized with gold nanoparticles (Au NPs) to construct an electrochemiluminescence (ECL) immunosensor. The prepared Au NP-functionalized g-C3N4 NS nanohybrids (Au-g-C3N4 NHs) exhibit strong and stable cathodic ECL activity compared to g-C3N4 NSs due to the important roles of Au NPs in trapping and storing the electrons from the conduction band of g-C3N4 NSs, as well as preventing high energy electron-induced passivation of g-C3N4 NSs. On the basis of the improved ECL stability and ECL peak intensity of the Au-g-C3N4 NHs, a novel ECL immunosensor was developed to detect carcinoembryonic antigen (CEA) as a model target analyte. The ECL immunosensor has a sensitive response to CEA in a linear range of 0.02–80 ng mL–1 with a detection limit of 6.8 pg mL–1. Additionally, the proposed immunosensor shows high specificity, good reproducibility, and long-term stability.
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