Šume hrasta crnike u Hrvatskoj nalaze u raznim degradacijskim stadijima, a jedan od njih je i makija koja je nepovoljniji stadij za klijanje sjemenki hrasta crnike u usporedbi sa crnikovim panjačama ili sastojinama visokog uzgojnog oblika. Crnika je kserofitna vrsta šumskog drveća. Geološku podlogu na kojoj dolazi crnika čine vapnenci i dolomiti koji su i najzastupljeniji na našem sredozemnom području. Crnika počinje cvjetati u osmoj godini života, rodi sjemenom u dobi 12. do 15. godine, a puni urod je svake 4. do 6. godine. Urod žira hrasta crnike je varijabilan, gdje su u osmogodišnjem periodu zabilježena tri obilna uroda žira. Puni urod hrasta crnike ovisi o klimatskim prilikama, ali se značajno razlikuje i između različitih staništa, posebice s obzirom na geološku podlogu i tip tla. Za laboratorijske analize, žir hrasta crnike sakupljen je na području G.J. „Kamenjak“ na otoku Rabu. Navedenom G.J. upravljaju „Hrvatske šume d.o.o.“. Sjeme je sakupljeno u odijelima 15a, 16a, 26a i 27a u periodu od 01. 12. 2020. do 10. 01. 2021. Sakupljanje je obavljeno metodom stresanja žira sa stabala na postavljene prostirke ispod krošanja i metodom sakupljanja otpalog žira s tla. Procjena vitaliteta sjemena metodom tetrazola i laboratorijske klijavosti napravljena je prema ISTA pravilima za hrast crniku. Postotak laboratorijske klijavosti utvrđen je prema postotku pravilnih klijanaca koji su normalno proklijali nakon 35. dana ispitivanja. Ukupni vitalitet žira hrasta crnike bio je visok i iznosio je 90,25 %, dok su ostatak od 9,75 % činile nevitalne sjemenke. Od vitalnog sjemena najveći postotak (79,50 %) čini sjeme kojemu je potpuno obojen embrij i kotiledoni. Žira s kotiledonima koji ima nekroze do 1/3 na distalnom dijelu, a nisu povezane sa embrionalnom šupljinom bilo je 10,75 %. U prvih sedam dana nije proklijao niti jedan žir pa je energija klijavosti iznosila 0,00 %. U prvih 14 dana laboratorijska klijavost je iznosila je 30,50 %, nakon 21. dana 76,50 %, nakon 28. dana 83,75 % te nakon 35. dana 88,50 %. Razlika između procjene vitaliteta žira i ukupne laboratorijske klijavosti iznosila je svega 1,75 %. Metoda procjene vitaliteta je brža i jeftinija od ispitivanja klijavosti. Rezultate procjene vitaliteta dobivamo za 18 sati, dok klijavost ispitujemo 28 ili 35 dana. Za praksu se može na osnovu rezultata ovih istraživanja predložiti ispitivanje vitaliteta žira hrasta crnike, a ne laboratorijske klijavosti kako preporučuju ISTA pravila (1993). Prosječno vrijeme klijanja (MGT) iznosilo je 19,91 dana. Pravilnih klijanaca bilo je 74,25 %, a nepravilnih 14,25 %. Na kraju ispitivanja utvrđeno je 8,50 % okularno zdravog žira koji nije proklijao dok je gnjilog žira bilo 3,00 %. Najčešća nepravilnost u iznosu od 56,14 % utvrđena je za kategoriju dva spojena klijanca, slijedi nekroza primarnih listova (12,28 %) i zakržljao primarni korijen (10,53 %). Ostale nepravilnosti pojavljuju se u manje od 10 % slučajeva. Holm oak forests in Croatia are in various stages of degradation, and one of them is maquis, which is a less favorable stage for the germination of holm oak seeds compared to holm oak stumps or stands of high growth form. Holm oak is a xerophytic species of forest trees. From the geological base, it comes from limestone, which is the most common in our Mediterranean area, and dolomite. Begins to flower in the eighth year of life, and the seed harvest begins at the age of 12 to 15 years, and the full harvest is every 4 to 6 years. The holm oak acorn crop is variable, where three abundant acorn crops were recorded in the eight-year period. The full harvest of holm oak depends on climatic conditions, but it also differs significantly between different habitats, especially the geological base and soil type. For laboratory analyses, holm oak acorns were collected in the area of G.J. "Kamenjak" on the island of Rab. The aforementioned G.J. managed by "Hrvatske šume d.o.o." The seeds were collected in department unit 15a, 16a, 26a and 27a in the period from 01/12/2020 to 10/01/2021. The collection was carried out using the method of shaking acorns from the trees onto mats placed under the canopy and the method of collecting fallen acorns from the ground. Assessment of seed vitality using the tetrazole method and laboratory germination was made according to the ISTA rules for holm oak. The percentage of laboratory germination was determined according to the percentage of normal seedlings that germinated normally after the 35th day of testing. The total vitality of holm oak acorns was high and amounted to 90.25 %, while the rest of 9.75 % consisted of non-vital seeds. Of the vital seeds, the largest percentage (79.50 %) consists of seeds with fully colored embryo and cotyledons. Acorns with cotyledons that has necrosis up to 1/3 on the distal part and are not connected to the embryonic cavity was 10.75 %. In the first seven days, not a single acorn germinated, so the germination energy was 0.00 %. In the first 14 days, laboratory germination was 30.50 %, after the 21st day 76.50 %, after the 28th day 83.75 % and after the 35th day 88.50 %. The difference between the assessment of acorn vitality and total laboratory germination was only 1.75 %. The vitality assessment method is faster and cheaper than the germination test. We get the results of the vitality assessment in 18 hours, while we test the germination after 28 or 35 days. Practical procedures can be based on the results of these studies suggest testing the vitality of the holm oak and not the laboratory germination as recommended by the ISTA rules. The average germination time (MGT) was 19.91 days. There were 74.25 % of regular seedlings and 14.25 % of irregular ones. At the end of the test, 8.50 % of the visually healthy acorns did not germinate, while 3.00 % of the rotten acorns were found. The most common irregularity in the amount of 56.14 % was determined for the category of two joined seedlings, followed by necrosis of primary leaves (12.28 %) and stunted primary root (10.53 %). Other irregularities appear in less than 10,00 % of cases.