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Ziv, Omer; Gabryelska, Marta M; Lun, Aaron T L; Gebert, Luca F R; Sheu-Gruttadauria, Jessica; Meredith, Luke W; Liu, Zhong-Yu; Kwok, Chun Kit; Qin, Cheng-Feng; MacRae, Ian J; Goodfellow, Ian; Marioni, John C; Kudla, Grzegorz; Miska, Eric A
Nature methods, 10/2018, Letnik: 15, Številka: 10Journal Article
The structural flexibility of RNA underlies fundamental biological processes, but there are no methods for exploring the multiple conformations adopted by RNAs in vivo. We developed cross-linking of matched RNAs and deep sequencing (COMRADES) for in-depth RNA conformation capture, and a pipeline for the retrieval of RNA structural ensembles. Using COMRADES, we determined the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs.
