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Engevik, Kristen A; Matthis, Andrea L; Montrose, Marshall H; Aihara, Eitaro
Methods in molecular biology, 2018, Letnik: 1734Journal Article
The advent of the gastric organoid culture system has provided a new model to emulate native epithelial tissue in vitro. Gastric organoids grow from isolated epithelial stem cells and develop into three dimensional structures that can be used to study host physiology. Here we describe current laboratory protocols for growing gastric organoids and the microinjection of pathogens such as Helicobacter pylori into the lumen of gastric organoids in order to study the cellular response following infection.
