One-pot dynamic kinetic resolution (DKR) has garnered considerable attention for its potential to generate enantiomerically pure compounds in high yields and simplifying operation. However, the challenge lies in achieving compatibility between enzymes and metals due to their distinct operating conditions. In this study, we present a novel cascade catalytic platform for the DKR of amines to address this issue. Pd nanoparticles were immobilized on lysine-decorated lipase nanogels, forming isolated metal-enzyme catalysts. The microwave-assisted one-pot DKR of amines was carried out under non-pressurized conditions at 50 °C for 5 h. The metal-enzyme nanogel biochemical composite exhibited excellent performances, with a high conversion rate of 98% and enantiomeric excess values exceeding 99%. This innovative approach demonstrates the potential of constructing a multifunctional metalloenzyme catalytic platform with a distinctive three-dimensional structure, which offers promising prospects for DKR applications. The metal-enzyme nanogel biochemical composite exhibits excellent catalytic performance in non-pressurized microwave conditions at 50 °C, achieving an outstanding 98% conversion rate and ee value＞99%. Display omitted •A novel Pd@CALB-Lys-nanogels cascade catalytic platform was developed.•Lysine modification reduces the size of enzyme nanogels and can promotes the dispersion of metal.•The catalyst exhibited high activity and selectivity in microwave-assisted Dynamic Kinetic Resolution.•The catalyst can spontaneously catalyze reactions at mild temperatures without the need for hydrogen pressure.