Raman spectroscopy and surface‐enhanced Raman spectroscopy (SERS) have been widely applied for characterizing biological samples. Nowadays, it is routine to use plastic products for biological sample preparation and analysis during spectral acquisition. However, plastic containers produce undesirable signals severely interfering with the spectra of biological samples, which is neglectable and misleading. Herein, we unveiled the physical and chemical interference of plastic products in the process of Raman spectroscopy characterization. Firstly, we revisited a representative case of interference from polystyrene plastics on normal Raman and SERS spectra of cysteine and demonstrated its physical interference due to misfocusing. Furthermore, we testified SERS spectra of aqueous solution stored in several plastic materials commonly used in biological experiments. The spectra indicate strong spectral interference from the leaching chemicals. To address these problems, we proposed a series of strategies to avoid interference from plastic products in the Raman and SERS characterization of biological samples. Plastic containers produce undesirable signals severely interfering with the spectra of biological samples, which is neglectable and misleading. In this work, a systematic and comprehensive understanding of how to avoid the impact of plasticware on Raman experiments is proposed to ensure the accuracy and reliability of these measurements.