This manuscript discusses the phytochemical analysis of stinging plants and/or their biological toxins as novel future bio-threat agents that may be used for self-defence purpose. The selected stinging plants namely, Urtica dioica L., Tragia involucrata L., Carduus nutans L. and Mucuna pruriens (L.) DC, have dual role as nutraceutical and ethno-pharmacological uses apart from their less-explored stinging property. The phytochemical analyses of their secondary metabolites including phenolics, terpene and N-containing compounds were measured. M. pruriens showed maximum total phenolic content (~1004 µg g −1 dry wt) followed by those of T. involucrata , C. nutans and U. dioica . Similarly, the flavonoid content was also found highest (~500 µg of QE g −1 dry wt) in M. pruriens . However, the tannin content was maximum (~654 µg g −1 dry wt) in T. involucrata followed by M. pruriens . Likewise, the terpenoid content was maximum (~350 µg of Lil g −1 dry wt) in U. dioica followed by M. pruriens , T. involucrata and C. nutans . FRSA was maximum (~31%) in M. pruriens . However, the oil content was highest in T. involucrata followed by U. dioica , M. pruriens and C. nutans . In addition, the alkaloid content was maximum (~523 µg of AE g −1 dry wt) in M. pruriens . These findings clearly suggest that M. pruriens has higher potential for harvesting of toxic compounds as compared to other studied plants for the formulation of novel future bio-threat agents for self-defence. However, detailed work is required for identification of the precise stinging chemical components.