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Sang, GuangMing; Luo, ShiNeng; Lin, JianGuo; Qiu, Ling; Chen, ChuanQing; Yang, HaiLin; Xia, YongMei
Science China Chemistry, 05/2010, Letnik: 53, Številka: 5Journal Article
A facile procedure was described for the hapten design of the N -methylcarbamate insecticide propoxur. Two new haptens of propoxur (hapten 1a and hapten 1b ) were synthesized by introducing appropriate spacers in the pesticide aromatic moiety of the analyte molecular structure. First, the propoxur reacted with nitric acid to yield the intermediate product. Then hapten 1a was prepared via the reduction of the intermediate product, and hapten 1b was formed by the acylation of hapten 1a with succinic anhydride. In addition, we have also developed a simple method to prepare hapten 2 for the pyrethroid insecticide cyhalothrin, which only requires two steps including the reactions of nitration and reduction. Three haptens were coupled to carrier proteins to prepare the corresponding artificial antigens. The molecular structures of these three haptens were identified by 1 H NMR and MS, and those of the artificial antigens were confirmed by UV-vis.
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