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Van Le, B.u.i.; Cruz de Carvalho, Maria H.; Zuily-Fodil, Yasmine; Thi, Anh T. Pham; Van, Kiem Tran Thanh
Journal of plant physiology, 11/2002, Letnik: 159, Številka: 11Journal Article
A simple protocol was established for high frequency direct shoot regeneration of cowpea Vigna unguiculata (L.) cv. EPACE-1. Bud proliferation occurred at the cotyledonary nodes of cowpea seedlings three weeks after culture on a medium containing Murashige and Skoog salts (1962) and B5 vitamins (Gamborg et al. 1968) supplemented with TDZ. A 10 μmol/L TDZ pre-treatment, shoot tip removal and excision of longitudinal thin cell layers (TCL) at the level of the cotyledonary nodes with subsequent culture on a MSB5 medium supplemented with 1 μmol/L IBA and 1 μmol/L TDZ were the optimal conditions for maximum bud proliferation. Up to 32.5 regenerated shoot buds were produced per TCL. The regenerated plants (R 0) were true-to-type and successfully transferred to soil.
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Vir: Osebne bibliografije
in: SICRIS
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