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Hobson, Claire Amaris; Bonacorsi, Stéphane; Jacquier, Hervé; Choudhury, Alaksh; Magnan, Mélanie; Cointe, Aurélie; Bercot, Béatrice; Tenaillon, Olivier; Birgy, André
Antimicrobial agents and chemotherapy, 11/2020, Letnik: 64, Številka: 12Journal Article
To explore the mutational possibilities of insertions and deletions (indels) in the carbapenemase (KPC) beta-lactamase, we selected for ceftazidime-avibactam-resistant mutants. Of 96 screened mutants, we obtained 19 indels (2 to 15 amino acids), all located in the loops surrounding the active site. Three antibiotic susceptibility phenotypes emerged: an extended-spectrum-beta-lactamase-like phenotype, an activity restricted to ceftazidime, and a carbapenem-susceptible KPC-like phenotype. Tolerance for indels reflects the evolvability of KPC beta-lactamase, which could challenge the therapeutic management of patients.
