•A flexible graphene-based platform for sensitive and selective non-enzymatic detection of H2O2.•Fully integration and customization with easy manufacturing process using laser irradiation.•A suitable transducer for migration of electrochemical sensors into areas where flexibility is required. Here, we demonstrate the detection of H2O2 with a novel, flexible and non-enzymatic electrochemical sensor based on a laser scribed graphene electrode decorated with silver nanoparticles(LSG-Ag). This low-cost technique based on a DVD laser, allowed the direct manufacturing and reduction of graphene oxide into an electrochemical transducer with conductive, lightweight, reduced and flexible characteristics. The LSG-Ag sensor showed a high catalytic response towards H2O2 reduction at -0.5 V with a fast amperometric response of only 3 s. The sensor exhibited a wide linear range from 0.1 to 10 mM (R2 = 0.9982), a low LOD of 7.9 μM with high repeatability of 3% R.S.D. and reproducibility of 4.5% R.S.D. In addition, the LSG-Ag retained its operational characteristics after continuous bending with a loss of current of only 13%. Considering the need of progression of electrochemical systems into trending areas where flexibility is required, the laser-induced sensors can provide a suitable platform for flexible, reliable, sensitive and selective detection.