An analytical method was developed by using LC-ESI(−ve)-MS/MS to investigate the residue dynamics of 2,4-D (2,4-dichlorophenoxyacetic acid) in green tea leaves, processed tea, tea liquor, and tea-cropped soil at Singhiajhora Tea Estate and Putinbari Tea Estate at Terai Region, Darjeeling District, West Bengal, India. In this method, an acidified methanol was used for extraction and subsequent clean-up was done by HLB (hydrophilic lipophilic balanced) cartridges. The method was validated as per SANTE guideline (SANTE/11813/2017). The limit of quantification (LOQ) of 2,4-D was 0.05 mgkg −1 and average % recoveries were in the range from 88.05 to 113.28 with relative standard deviation (RSD) 3.46 to 6.43. The dissipation of 2,4-D followed the 1st-order reaction kinetics with a half-life ( T 1/2 ) of 1.51–1.61 day at the recommended dose and 2.50–2.72 day for doubled recommended dose in tea for both locations. This method can be applied successfully for the determination of 2,4 D residues in/on tea matrix and subsequent studies on safety evaluation showed that the use of 2,4-D in tea is safe.