Rivers get polluted with diverse types of hazardous and toxic substances, pesticides being one of them. The water and sediment of rivers get contaminated with pesticide residues coming through the run-off of vast agricultural fields along the catchment area and also from domestic sewage water. The residues get bio-concentrated and bio-accumulated in different aquatic organisms and animals including fishes along the food chain. Fish, one of the important and chief sources of proteins, are consumed by humans. The presence of toxic substances like pesticides in any food item is undesirable for the fear of health hazards. We have monitored the status of pesticide residue in river Gomti, a tributary of River Ganga that passes through the Uttar Pradesh state of India. Water, sediment, and fish samples collected from the different locations along the river stretch were analyzed for 34 targeted pesticide compounds belonging to organochlorines (OC), organophosphates (OP), and synthetic pyrethroids (SP) groups. In 52% of water, 30% of sediment, and 43% of fish samples residues of OCs were detected while the OPs were present in 33%, 25%, and 39% of samples respectively. However, none of the SPs could be recorded in any sample. The concentrations of the pesticides in water indicate stress conditions to some extent to aquatic life, but based on the human health risk assessment it can be concluded that consumption of fishes from the river contaminated with different OC or OP residues would not pose any direct risk to the consumers.