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Wong-Arce, Alejandra; Gonzalez-Ortega, Omar; Romero-Maldonado, Andrea; Miranda-López, Arleth; García-Soto, Mariano; Farfán-Castro, Susan; Betancourt-Mendiola, Lourdes; Teeravechyan, Samaporn; Srisutthisamphan, Kanjana; Comas-García, Mauricio; Solís Andrade, Karla I; Rosales-Mendoza, Sergio
Pharmaceuticals (Basel, Switzerland), 02/2024, Letnik: 17, Številka: 3Journal Article
Subunit vaccines stand as a leading approach to expanding the current portfolio of vaccines to fight against COVID-19, seeking not only to lower costs but to achieve long-term immunity against variants of concern and have the main attributes that could overcome the limitations of the current vaccines. Herein a chimeric protein targeting S1 and S2 epitopes, called LTp50, was designed as a convenient approach to induce humoral responses against SARS-CoV-2. LTp50 was produced in recombinant using a conventional pET vector, recovering the expected antigen in the insoluble fraction. LTp50 was purified by chromatography (purity > 90%). The solubilization and refolding stages helped to obtain a stable protein amenable for vaccine formulation. LTp50 was adsorbed onto alum, resulting in a stable formulation whose immunogenic properties were assessed in BALB/c mice. Significant humoral responses against the S protein (BA.5 variant) were detected in mice subjected to three subcutaneous doses (10 µg) of the LTp50/alum formulation. This study opens the path for the vaccine formulation optimization using additional adjuvants to advance in the development of a highly effective anti-COVID-19 vaccine directed against the antigenic regions of the S protein, which are less prone to mutations.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Vir: Osebne bibliografije
in: SICRIS
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