E-viri
-
Davis-Vogel, Courtney A
Methods in molecular biology (Clifton, N.J.), 2022, Letnik: 2360Journal Article
Reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays are a highly accurate and precise method for measuring transcript expression levels. A major drawback of RT-qPCR is the extensive optimization and validation necessary to produce high-quality assays, as described in the guidelines "Minimum Information for Publication of Quantitative Real-Time PCR Experiments." This chapter describes use of designed and optimized RT-qPCR assays that accurately detect expression of eight genes predicted to be centrally involved in the RNA interference (RNAi) pathways of western corn rootworm (WCR), and appropriate accompanying parameters. Assay gene targets include drosha, dicer-1, dicer-2, pasha, loquacious, r2d2, argonaute 1, and argonaute 2, and detection has been validated at nine different points in the WCR life cycle. These assays can be used with this procedure to assess expression of any one of these core RNAi pathway genes in up to 96 samples per 384-well qPCR plate.
Avtor
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.