Objective: This study investigated the cardiotoxicity of cisplatin (CIS) on rat heart by using the oxidative damage of the rat myocardium, troponin I and serum S100A1 levels. Previous studies have reported that cell-protective effect of Pycnogenol® (PYC) depended on its antioxidant and anti-inflammatory properties. Hence, the myocardial protective effect of PYC was investigated in this study. Materials and Methods: Rats were randomly grouped to four with 5 rats in each group. The groups were consisted of control group, PYC group: 10 mg/kg PYC for 7 days, CIS group: 15 mg/kg single injection of CIS on the 5th day, CIS + PYC group: 10 mg/kg PYC for 7 days, plus 15 mg/kg single injection of CIS on the 5th day. Heart and serum samples were acquired subsequently. Results: CIS and PYC co-treatment group had increased catalase level (from 43.61±15.16 to 60.80±21.36, p<0.019) and prevented troponin I elevation (from 7.34±6.20 to 3.03±1.36). The S100A1 level was significantly reduced by CIS (from 10.25±8.8 to 3.99±2.87, p<0.035) and was restored by PYC treatment (32.07±29.23). Conclusion: Injured cardiomyocytes released troponin I after exposure to CIS and PYC, which can protect the cells from CIS cardiotoxicity, increased the tissue catalase level. Additionally, PYC treatment increased serum level of S100A1.