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Shiraishi, Takumi; Inui, Shogo; Inoue, Yuta; Saito, Yumiko; Taga, Hideto; Kaneko, Masatomo; Tsuji, Keisuke; Ueda, Saya; Ueda, Takashi; Matsugasumi, Toru; Taniguchi, Hidefumi; Ueno, Akihisa; Yamada, Takeshi; Yamada, Yasuhiro; Iwata, Tsuyoshi; Fujihara, Atsuko; Hongo, Fumiya; Ukimura, Osamu
Scientific reports, 10/2020, Letnik: 10, Številka: 1Journal Article
We developed a novel dividing device that can split needle biopsy tissues along longitude axis aiming to achieve definitive molecular-biological and genetical analysis with reference of pathological diagnosis of the side-by-side divided tissue as spatially matched information. The aim of this study was to evaluate the feasibility and potential usefulness of the novel dividing device to provide the appropriate materials for molecular diagnosis. The new device was examined using mouse xenograft tumors. Real-time quantitative PCR and genetic test were performed to evaluate the feasibility and usefulness of the device. All the samples from needle biopsy were successfully divided into two pieces. Quality and quantity from divided samples harbor high enough to perform gene expression analysis (real-time PCR) and genetic test. Using two divided samples obtained from xenograft tumor model by needle biopsy, the % length of xenograft tumor (human origin) was significantly correlated with the % human genomic DNA (p = 0.00000608, r = 0.987), indicating that these divided samples were spatially matched. The novel longitudinally dividing device of a needle biopsy tissue was useful to provide the appropriate materials for molecular-biological and genetical analysis with reference of pathological diagnosis as spatially matched information.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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