Timely transfusion of blood products is crucial, particularly in critical medical situations. Inadequate blood donation rates pose a significant threat to public health, potentially compromising patient care. This study aims to investigate the attitudes and willingness of Syrian college students to engage in blood donation, assess the prevalence of voluntary blood donation, evaluate their understanding of the determinants and procedures involved in the donation process, and identify the key factors influencing their behavior. A cross-sectional study was conducted employing a self-administered questionnaire, distributed in both electronic and hard copy formats among college students. Data analysis was performed using the R Statistical Software. Results were presented in terms of odds ratios (OR), with statistical significance defined at a P value of .05 and a 95% confidence interval. A total of 673 medical students participated in this study, with an average age of 21.6 years. The majority (59.2%) reported a good economic status, and a high level of knowledge (99.7%). A substantial proportion (40.4%) agreed with the notion that blood donation constitutes a personal duty for every eligible individual. Multiple comparison tests revealed a statistically significant P value of .0009716, specifically concerning individuals with low economic status. Our study demonstrates a positive attitude among college students towards voluntary blood product donation. However, the majority have not engaged in voluntary activities unless mandated for specific certification purposes. Donation rates were relatively higher than the reported rates in other similar studies conducted in Middle Eastern countries.